Gene description for Emilin1
Gene name elastin microfibril interfacer 1
Gene symbol Emilin1
Other names/aliases 5830419M17Rik
AW229038
BB105748
EMILIN-1
gp115
Species Mus musculus
 Database cross references - Emilin1
ExoCarta ExoCarta_100952
Vesiclepedia VP_100952
Entrez Gene 100952
UniProt Q99K41  
 Emilin1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Emilin1
Molecular Function
    protein binding GO:0005515 IPI
    extracellular matrix constituent conferring elasticity GO:0030023 IBA
    extracellular matrix constituent conferring elasticity GO:0030023 IMP
    extracellular matrix constituent conferring elasticity GO:0030023 RCA
    identical protein binding GO:0042802 IDA
    identical protein binding GO:0042802 ISO
    molecular adaptor activity GO:0060090 IEA
    molecular adaptor activity GO:0060090 ISO
    integrin binding involved in cell-matrix adhesion GO:0098640 IEA
    integrin binding involved in cell-matrix adhesion GO:0098640 ISO
Biological Process
    aortic valve morphogenesis GO:0003180 IMP
    cell-matrix adhesion GO:0007160 IEA
    cell-matrix adhesion GO:0007160 ISO
    regulation of blood pressure GO:0008217 IDA
    positive regulation of gene expression GO:0010628 IMP
    negative regulation of gene expression GO:0010629 IMP
    positive regulation of cell-substrate adhesion GO:0010811 IDA
    cell migration GO:0016477 IEA
    cell migration GO:0016477 ISO
    negative regulation of angiogenesis GO:0016525 IMP
    positive regulation of blood coagulation GO:0030194 ISS
    extracellular matrix organization GO:0030198 IDA
    negative regulation of cell migration GO:0030336 ISS
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 IMP
    negative regulation of vascular endothelial growth factor receptor signaling pathway GO:0030948 IMP
    negative regulation of collagen biosynthetic process GO:0032966 IMP
    cell adhesion mediated by integrin GO:0033627 IDA
    regulation of cell population proliferation GO:0042127 IMP
    positive regulation of apoptotic process GO:0043065 ISS
    positive regulation of angiogenesis GO:0045766 IDA
    elastic fiber assembly GO:0048251 IMP
    negative regulation of cell activation GO:0050866 IMP
    negative regulation of SMAD protein signal transduction GO:0060392 IMP
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IMP
    positive regulation of defense response to bacterium GO:1900426 ISO
    negative regulation of vascular endothelial growth factor signaling pathway GO:1900747 IMP
    positive regulation of extracellular matrix assembly GO:1901203 IMP
    positive regulation of platelet aggregation GO:1901731 ISS
    negative regulation of collagen fibril organization GO:1904027 IMP
    negative regulation of macrophage migration GO:1905522 IMP
Subcellular Localization
    extracellular region GO:0005576 TAS
    collagen trimer GO:0005581 IEA
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 ISO
    extracellular matrix GO:0031012 IDA
    integrin alpha4-beta1 complex GO:0034668 IEA
    integrin alpha4-beta1 complex GO:0034668 ISO
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 ISO
    collagen-containing extracellular matrix GO:0062023 ISS
    collagen-containing extracellular matrix GO:0062023 NAS
    EMILIN complex GO:1990971 ISO
 Experiment description of studies that identified Emilin1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1101
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 1102
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
4
Experiment ID 1103
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Emilin1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PRKAA1 5562
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which Emilin1 is involved
PathwayEvidenceSource
Elastic fibre formation IEA Reactome
Extracellular matrix organization IEA Reactome
Molecules associated with elastic fibres IEA Reactome





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