Gene description for ATP8A1
Gene name ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1
Gene symbol ATP8A1
Other names/aliases ATPASEII
ATPIA
ATPP2
Species Homo sapiens
 Database cross references - ATP8A1
ExoCarta ExoCarta_10396
Entrez Gene 10396
HGNC 13531
MIM 609542
UniProt Q9Y2Q0  
 ATP8A1 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for ATP8A1
Molecular Function
    ATP binding GO:0005524 IEA
    cation-transporting ATPase activity GO:0019829 NAS
    magnesium ion binding GO:0000287 IEA
    phospholipid-translocating ATPase activity GO:0004012 IBA
    protein binding GO:0005515 IPI
Biological Process
    metabolic process GO:0008152 IEA
    learning GO:0007612 IEA
    ion transmembrane transport GO:0034220 TAS
    positive regulation of phospholipid translocation GO:0061092 IEA
    positive regulation of cell migration GO:0030335 IEA
    cation transmembrane transport GO:0098655 NAS
    cation transport GO:0006812 NAS
    transmembrane transport GO:0055085 TAS
    phospholipid translocation GO:0045332 NAS
Subcellular Localization
    chromaffin granule membrane GO:0042584 IEA
    plasma membrane GO:0005886 TAS
    integral component of membrane GO:0016021 IEA
    Golgi apparatus GO:0005794 IDA
    membrane GO:0016020 IDA
    extracellular exosome GO:0070062 IDA
    endoplasmic reticulum GO:0005783 IDA
 Experiment description of studies that identified ATP8A1 in exosomes
1
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ATP8A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ATP8A1 is involved
PathwayEvidenceSource
Ion transport by P-type ATPases TAS Reactome





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