ExoCarta: Gene summary

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Gene description for NUP43
Gene name	nucleoporin 43kDa
Gene symbol	NUP43
Other names/aliases	bA350J20.1 p42
Species	Homo sapiens
Database cross references - NUP43
ExoCarta	ExoCarta_348995
Vesiclepedia	VP_348995
Entrez Gene	348995
HGNC	21182
MIM	608141
UniProt	Q8NFH3
NUP43 identified in exosomes derived from the following tissue/cell type
Chondrocytes	35931686
Chondrocytes	35931686
Mesenchymal stem cells	36408942
Ovarian cancer cells	23333927
Ovarian cancer cells	23333927

Gene ontology annotations for NUP43

Molecular Function
	protein binding	GO:0005515	IPI
Biological Process
	nucleocytoplasmic transport	GO:0006913	NAS
	chromosome segregation	GO:0007059	IEA
	protein transport	GO:0015031	IEA
	mRNA transport	GO:0051028	IEA
	cell division	GO:0051301	IEA
Subcellular Localization
	kinetochore	GO:0000776	IDA
	nuclear envelope	GO:0005635	IDA
	nuclear envelope	GO:0005635	TAS
	nuclear pore	GO:0005643	NAS
	nucleoplasm	GO:0005654	IDA
	cytosol	GO:0005829	IDA
	cytosol	GO:0005829	TAS
	nuclear speck	GO:0016607	IDA
	nuclear pore outer ring	GO:0031080	IBA
	nuclear pore outer ring	GO:0031080	IDA
	nuclear pore outer ring	GO:0031080	NAS

Experiment description of studies that identified NUP43 in exosomes

Experiment ID

497

MISEV standards
✘	Biophysical techniques
✔ CD9\|CD151\|CD63\|CD81\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|RAB5A\|SDCBP\|TFRC\|TSG101\|UCHL1	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

"Varela-EirÃn M, Carpintero-FernÃ¡ndez P, GuitiÃ¡n-CaamaÃ±o A, Varela-VÃ¡zquez A, GarcÃa-Yuste A, SÃ¡nchez-Temprano A, Bravo-LÃ³pez SB, YaÃ±ez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, MayÃ¡n MD."

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

T/C-28a2

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

498

MISEV standards
✔ EM	Biophysical techniques
✔ CD9\|CD151\|CD63\|CD81\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|RAB5A\|SDCBP\|TFRC\|TSG101\|UCHL1	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

T/C-28a2

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

488

MISEV standards
✔ EM	Biophysical techniques
✔ CD9\|CD81\|CD63\|GAPDH\|SDCBP\|LAMP1\|TFRC\|UCHL1\|FLOT2\|LAMP2\|FLOT1\|ICAM1\|RAB5B\|CD151\|RAB35\|TSG101\|RAB5A\|CD82	Enriched markers
✔ CANX	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Homo sapiens

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Mesenchymal stem cells

Sample name

UCMSC

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

211

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|Alix\|EpCAM\|TFRC	Enriched markers
✔ cytochrome c\|GOLGA2	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23333927

Organism

Homo sapiens

Experiment description

Characterization and proteomic analysis of ovarian cancer-derived exosomes.

Authors

"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."

Journal name

J Proteomics

Publication year

2013

Sample

Ovarian cancer cells

Sample name

IGROV1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.09-1.15 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

212

MISEV standards
✔ CEM	Biophysical techniques
✔ TSG101\|Alix\|EpCAM\|TFRC	Enriched markers
✔ Cytochrome C\|GOLGA2	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23333927

Organism

Homo sapiens

Experiment description

Characterization and proteomic analysis of ovarian cancer-derived exosomes.

Authors

"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."

Journal name

J Proteomics

Publication year

2013

Sample

Ovarian cancer cells

Sample name

OVCAR-3

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.09-1.15 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Protein-protein interactions for NUP43

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

EIF4G3

8672

Proximity Label-MS

Homo sapiens

SLFN11

91607

Affinity Capture-MS

Homo sapiens

RIT1

6016

Negative Genetic

Homo sapiens

ZNF292

Proximity Label-MS

Homo sapiens

NCOA6

Proximity Label-MS

Homo sapiens

HPS5

11234

Proximity Label-MS

Homo sapiens

ISG15

9636

Affinity Capture-MS

Homo sapiens

HOOK1

Proximity Label-MS

Homo sapiens

KLC2

64837

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CCT3

7203

Proximity Label-MS

Homo sapiens

NUPL2

11097

Proximity Label-MS

Homo sapiens

JMJD1C

221037

Proximity Label-MS

Homo sapiens

HSPA6

3310

Proximity Label-MS

Homo sapiens

Rcc1

Affinity Capture-MS

Mus musculus

ANXA3

306

Proximity Label-MS

Homo sapiens

ZDHHC7

Proximity Label-MS

Homo sapiens

DDTL

Proximity Label-MS

Homo sapiens

ZNF703

Proximity Label-MS

Homo sapiens

KPNB1

3837

Affinity Capture-MS

Homo sapiens

ACTC1

Proximity Label-MS

Homo sapiens

ARL6IP4

Proximity Label-MS

Homo sapiens

XPA

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

GID4

Proximity Label-MS

Homo sapiens

UBC

7316

Affinity Capture-MS

Homo sapiens

POM121

Proximity Label-MS

Homo sapiens

NETO1

Proximity Label-MS

Homo sapiens

CCT6B

10693

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ATP8A1

10396

Proximity Label-MS

Homo sapiens

EIF1

10209

Proximity Label-MS

Homo sapiens

RILPL1

353116

Proximity Label-MS

Homo sapiens

ELF4

2000

Proximity Label-MS

Homo sapiens

PCBP3

54039

Affinity Capture-MS

Homo sapiens

NUP160

23279

Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

EIF4ENIF1

56478

Proximity Label-MS

Homo sapiens

RUVBL1

8607

Proximity Label-MS

Homo sapiens

NCOR1

Proximity Label-MS

Homo sapiens

C11orf30

Proximity Label-MS

Homo sapiens

IFT74

80173

Proximity Label-MS

Homo sapiens

MIR1236

Proximity Label-MS

Homo sapiens

SENP1

Proximity Label-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

NUP153

9972

Affinity Capture-MS		Homo sapiens
Proximity Label-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

KIF5B

3799

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CCDC168

Proximity Label-MS

Homo sapiens

PRC1

9055

Affinity Capture-MS

Homo sapiens

MDC1

Proximity Label-MS

Homo sapiens

FBLN2

2199

Proximity Label-MS

Homo sapiens

NUP93

9688

Proximity Label-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

AK2

204

Proximity Label-MS

Homo sapiens

LACE1

Proximity Label-MS

Homo sapiens

DCTD

1635

Proximity Label-MS

Homo sapiens

MUC19

Proximity Label-MS

Homo sapiens

CAMK2B

816

Affinity Capture-MS

Homo sapiens

PPM1A

5494

Proximity Label-MS

Homo sapiens

ELP4

Proximity Label-MS

Homo sapiens

BCOR

Proximity Label-MS

Homo sapiens

YEATS2

Proximity Label-MS

Homo sapiens

NOC3L

64318

Proximity Label-MS

Homo sapiens

TUBA1A

7846

Proximity Label-MS

Homo sapiens

MAVS

57506

Proximity Label-MS

Homo sapiens

SNX27

81609

Affinity Capture-MS

Homo sapiens

DTYMK

1841

Proximity Label-MS

Homo sapiens

PFDN6

10471

Proximity Label-MS

Homo sapiens

CCT2

10576

Affinity Capture-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

UBE2V1

7335

Proximity Label-MS

Homo sapiens

E2F4

Affinity Capture-MS

Homo sapiens

SRP9

6726

Proximity Label-MS

Homo sapiens

POM121C

Proximity Label-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

TCP1

6950

Proximity Label-MS

Homo sapiens

ATG4A

Proximity Label-MS

Homo sapiens

SENP2

59343

Proximity Label-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

DBNL

28988

Proximity Label-MS

Homo sapiens

HIST1H4A

8359

Affinity Capture-MS

Homo sapiens

SUCLG1

8802

Proximity Label-MS

Homo sapiens

CCAR2

57805

Proximity Label-MS

Homo sapiens

SRSF3

6428

Proximity Label-MS

Homo sapiens

KLC4

89953

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

EIF2A

83939

Proximity Label-MS

Homo sapiens

MAPRE1

22919

Affinity Capture-MS

Homo sapiens

CCT5

22948

Proximity Label-MS

Homo sapiens

TTF1

Proximity Label-MS

Homo sapiens

PFDN2

5202

Proximity Label-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

CNOT1

23019

Proximity Label-MS

Homo sapiens

MIR1292

Proximity Label-MS

Homo sapiens

NUP62

23636

Proximity Label-MS

Homo sapiens

CLSTN2

64084

Affinity Capture-MS

Homo sapiens

LUC7L2

51631

Proximity Label-MS

Homo sapiens

BICD2

23299

Proximity Label-MS

Homo sapiens

LAGE3

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

AKR1A1

10327

Proximity Label-MS

Homo sapiens

SMARCE1

6605

Proximity Label-MS

Homo sapiens

Ube2i

Affinity Capture-MS

Mus musculus

AHCTF1

25909

Proximity Label-MS

Homo sapiens

CSTB

1476

Proximity Label-MS

Homo sapiens

NIPSNAP1

8508

Proximity Label-MS

Homo sapiens

CD93

Proximity Label-MS

Homo sapiens

Nup155

Affinity Capture-MS

Mus musculus

SYNE2

23224

Proximity Label-MS

Homo sapiens

LARP1

23367

Proximity Label-MS

Homo sapiens

ZFP36L2

678

Proximity Label-MS

Homo sapiens

100

BCLAF1

9774

Proximity Label-MS

Homo sapiens

101

DST

667

Proximity Label-MS

Homo sapiens

102

NUP37

79023

Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

103

GLOD4

51031

Proximity Label-MS

Homo sapiens

104

PGLS

25796

Proximity Label-MS

Homo sapiens

105

FANCD2

Affinity Capture-MS

Homo sapiens

106

MAP4

4134

Proximity Label-MS

Homo sapiens

107

Nup98

Affinity Capture-MS		Mus musculus
Affinity Capture-MS		Mus musculus

108

EIF4G1

1981

Proximity Label-MS

Homo sapiens

109

SEC13

6396

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

110

CCT6A

908

Proximity Label-MS

Homo sapiens

111

RLIM

51132

Affinity Capture-MS

Homo sapiens

112

HCFC1

3054

Proximity Label-MS

Homo sapiens

113

NUP88

4927

Proximity Label-MS

Homo sapiens

114

HSP90AB1

3326

Proximity Label-MS

Homo sapiens

115

STIM1

6786

Proximity Label-MS

Homo sapiens

116

EGFR

1956

Negative Genetic

Homo sapiens

117

OSGEP

55644

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

118

FUS

2521

Affinity Capture-MS

Homo sapiens

119

NUP214

8021

Proximity Label-MS

Homo sapiens

120

EIF4G2

1982

Proximity Label-MS

Homo sapiens

121

TNRC6B

Proximity Label-MS

Homo sapiens

122

UBL5

59286

Proximity Label-MS

Homo sapiens

123

VBP1

7411

Proximity Label-MS

Homo sapiens

124

TOX3

Proximity Label-MS

Homo sapiens

125

FSIP2

401024

Proximity Label-MS

Homo sapiens

126

ARID1B

57492

Proximity Label-MS

Homo sapiens

127

GRM7

2917

Proximity Label-MS

Homo sapiens

128

RBM26

64062

Proximity Label-MS

Homo sapiens

129

Nup107

Affinity Capture-MS		Mus musculus
Affinity Capture-MS		Mus musculus

130

NME1

4830

Proximity Label-MS

Homo sapiens

131

MTTP

4547

Proximity Label-MS

Homo sapiens

132

RGPD5

Affinity Capture-MS

Homo sapiens

133

ZMYND8

23613

Proximity Label-MS

Homo sapiens

134

HSDL2

84263

Proximity Label-MS

Homo sapiens

135

PHF21A

Proximity Label-MS

Homo sapiens

136

PDZD2

Proximity Label-MS

Homo sapiens

137

ELF2

Proximity Label-MS

Homo sapiens

138

HP1BP3

50809

Proximity Label-MS

Homo sapiens

139

TP53RK

112858

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

140

SPAST

6683

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

141

DDB1

1642

Affinity Capture-Western

Homo sapiens

142

AHNAK2

113146

Proximity Label-MS

Homo sapiens

143

RANBP1

5902

Affinity Capture-MS

Homo sapiens

144

ARID1A

8289

Proximity Label-MS

Homo sapiens

145

SEH1L

81929

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Reconstituted Complex		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

146

TPRKB

51002

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

147

CCDC88A

55704

Proximity Label-MS

Homo sapiens

148

HSPD1

3329

Proximity Label-MS

Homo sapiens

149

ASH1L

55870

Proximity Label-MS

Homo sapiens

150

CETN2

1069

Affinity Capture-Western

Homo sapiens

151

BAIAP2

10458

Proximity Label-MS

Homo sapiens

152

PFDN5

5204

Proximity Label-MS

Homo sapiens

153

RAE1

8480

Proximity Label-MS

Homo sapiens

154

ATP13A5

Proximity Label-MS

Homo sapiens

155

NKAP

79576

Proximity Label-MS

Homo sapiens

156

AVPR2

Proximity Label-MS

Homo sapiens

157

SUGT1

10910

Proximity Label-MS

Homo sapiens

158

FBN1

2200

Proximity Label-MS

Homo sapiens

159

SALL2

Proximity Label-MS

Homo sapiens

160

RRM2

6241

Proximity Label-MS

Homo sapiens

161

CCT8

10694

Proximity Label-MS

Homo sapiens

162

SIRT6

Affinity Capture-MS

Homo sapiens

163

PDCL3

79031

Proximity Label-MS

Homo sapiens

164

NUP133

55746

Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

165

RAB3GAP2

25782

Co-fractionation

Homo sapiens

166

SSFA2

6744

Proximity Label-MS

Homo sapiens

167

TP53

7157

Proximity Label-MS

Homo sapiens

168

MKL2

Proximity Label-MS

Homo sapiens

169

TMEM214

54867

Proximity Label-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

170

RGPD8

Affinity Capture-MS

Homo sapiens

171

ERH

2079

Proximity Label-MS

Homo sapiens

172

DNAH9

Proximity Label-MS

Homo sapiens

173

PDCL

5082

Proximity Label-MS

Homo sapiens

174

DNAJA2

10294

Proximity Label-MS

Homo sapiens

175

PIN1

5300

Proximity Label-MS

Homo sapiens

176

RYR2

6262

Proximity Label-MS

Homo sapiens

177

PSMB7

5695

Proximity Label-MS

Homo sapiens

178

PRRC2C

23215

Proximity Label-MS

Homo sapiens

179

COX5A

9377

Proximity Label-MS

Homo sapiens

180

NGEF

Proximity Label-MS

Homo sapiens

181

USP44

Proximity Label-MS

Homo sapiens

182

DCTN2

10540

Co-fractionation

Homo sapiens

183

LRP12

29967

Proximity Label-MS

Homo sapiens

184

SND1

27044

Proximity Label-MS

Homo sapiens

185

NUP98

4928

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Proximity Label-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

186

SMC1A

8243

Proximity Label-MS

Homo sapiens

187

CCT7

10574

Affinity Capture-MS

Homo sapiens

188

PSMA1

5682

Proximity Label-MS

Homo sapiens

189

Nup214

Affinity Capture-MS

Mus musculus

190

NUP50

10762

Proximity Label-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

191

NUP107

57122

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Proximity Label-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

192

TAGLN2

8407

Affinity Capture-MS

Homo sapiens

193

MT1F

Proximity Label-MS

Homo sapiens

194

TCF20

Proximity Label-MS

Homo sapiens

195

RANBP2

5903

Proximity Label-MS

Homo sapiens

196

LIN54

Proximity Label-MS

Homo sapiens

197

Ranbp2

Affinity Capture-MS

Mus musculus

198

LMLN

Proximity Label-MS

Homo sapiens

199

CUL7

9820

Affinity Capture-MS

Homo sapiens

200

KIAA1551

Proximity Label-MS

Homo sapiens

201

NUP85

79902

Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens
Reconstituted Complex		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

202

PLD6

Affinity Capture-MS

Homo sapiens

203

ZNF318

24149

Proximity Label-MS

Homo sapiens

204

TDRD7

23424

Proximity Label-MS

Homo sapiens

205

NR1D2

Proximity Label-MS

Homo sapiens

206

TIMM13

26517

Proximity Label-MS

Homo sapiens

207

CUL4A

8451

Affinity Capture-Western

Homo sapiens

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