Gene description for DDB1
Gene name damage-specific DNA binding protein 1, 127kDa
Gene symbol DDB1
Other names/aliases DDBA
UV-DDB1
XAP1
XPCE
XPE
XPE-BF
Species Homo sapiens
 Database cross references - DDB1
ExoCarta ExoCarta_1642
Entrez Gene 1642
HGNC 2717
MIM 600045
UniProt Q16531  
 DDB1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for DDB1
Molecular Function
    protein binding GO:0005515 IPI
    damaged DNA binding GO:0003684 IDA
    DNA binding GO:0003677 TAS
Biological Process
    negative regulation of apoptotic process GO:0043066 IEA
    DNA damage response, detection of DNA damage GO:0042769 TAS
    nucleotide-excision repair GO:0006289 TAS
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IMP
    histone H2A monoubiquitination GO:0035518 IDA
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 IDA
    viral process GO:0016032 IEA
    regulation of mitotic cell cycle phase transition GO:1901990 IMP
    DNA repair GO:0006281 TAS
    nucleotide-excision repair, DNA damage removal GO:0000718 TAS
    positive regulation of viral release from host cell GO:1902188 IMP
    positive regulation of viral genome replication GO:0045070 IMP
    UV-damage excision repair GO:0070914 IDA
    interaction with symbiont GO:0051702 IDA
    positive regulation by virus of viral protein levels in host cell GO:0046726 IMP
    translesion synthesis GO:0019985 TAS
    Wnt signaling pathway GO:0016055 IEA
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    extracellular space GO:0005615 IDA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    Cul4-RING E3 ubiquitin ligase complex GO:0080008 IDA
    Cul4A-RING E3 ubiquitin ligase complex GO:0031464 IDA
    Cul4B-RING E3 ubiquitin ligase complex GO:0031465 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified DDB1 in exosomes
1
Experiment ID 234
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for DDB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DDB2  
Affinity Capture-Western Homo sapiens
2 RFWD2  
Affinity Capture-Western Homo sapiens
3 DET1  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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