Gene description for DYNLL1
Gene name dynein, light chain, LC8-type 1
Gene symbol DYNLL1
Other names/aliases DLC1
DLC8
DNCL1
DNCLC1
LC8
LC8a
PIN
hdlc1
Species Homo sapiens
 Database cross references - DYNLL1
ExoCarta ExoCarta_8655
Entrez Gene 8655
HGNC 15476
MIM 601562
UniProt P63167  
 DYNLL1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Hepatocellular carcinoma cells 26054723    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for DYNLL1
Molecular Function
    protein binding GO:0005515 IPI
    nitric-oxide synthase regulator activity GO:0030235 IEA
    protein homodimerization activity GO:0042803 IEA
    enzyme binding GO:0019899 IEA
    motor activity GO:0003774 IEA
Biological Process
    apoptotic process GO:0006915 TAS
    microtubule-based process GO:0007017 IEA
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 TAS
    transcription, DNA-templated GO:0006351 IEA
    positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway GO:1900740 TAS
    organelle organization GO:0006996 TAS
    regulation of transcription, DNA-templated GO:0006355 IEA
    intrinsic apoptotic signaling pathway GO:0097193 TAS
    programmed cell death GO:0012501 TAS
    female gamete generation GO:0007292 TAS
    regulation of catalytic activity GO:0050790 IEA
    substantia nigra development GO:0021762 IEP
    anatomical structure morphogenesis GO:0009653 TAS
    G2/M transition of mitotic cell cycle GO:0000086 TAS
    mitotic cell cycle GO:0000278 TAS
    actin cytoskeleton organization GO:0030036 TAS
    transport GO:0006810 IEA
    negative regulation of phosphorylation GO:0042326 IDA
    viral process GO:0016032 IEA
Subcellular Localization
    COP9 signalosome GO:0008180 IDA
    mitochondrion GO:0005739 IEA
    membrane GO:0016020 IDA
    cytosol GO:0005829 TAS
    primary cilium GO:0072372 TAS
    ciliary tip GO:0097542 TAS
    centrosome GO:0005813 IDA
    cytoplasmic dynein complex GO:0005868 ISS
    nucleus GO:0005634 IDA
    kinetochore GO:0000776 IDA
    microtubule GO:0005874 IEA
    mitotic spindle GO:0072686 IDA
    cytoplasm GO:0005737 IDA
    plasma membrane GO:0005886 TAS
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified DYNLL1 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 234
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
5
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DYNLL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TP53BP1  
Two-hybrid Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
2 Map1lc3b 64862
Affinity Capture-MS Rattus norvegicus
3 Polh  
Affinity Capture-MS Mus musculus
4 Grin2a  
Affinity Capture-MS Rattus norvegicus
5 Actg1 287876
Affinity Capture-MS Rattus norvegicus
6 Car1 310218
Affinity Capture-MS Rattus norvegicus
7 NDUFA4L2  
Two-hybrid Homo sapiens
8 THAP8  
Two-hybrid Homo sapiens
9 1300007L22Rik  
Affinity Capture-MS Mus musculus
10 BMF  
Reconstituted Complex Homo sapiens
11 C14orf1 11161
Two-hybrid Homo sapiens
12 Mastl predicted  
Affinity Capture-MS Rattus norvegicus
13 EEF1A1 1915
Two-hybrid Homo sapiens
14 Tuba1a 64158
Affinity Capture-MS Rattus norvegicus
15 Mast1  
Affinity Capture-MS Mus musculus
16 Cyln2  
Affinity Capture-MS Rattus norvegicus
17 ZHX1  
Two-hybrid Homo sapiens
18 NEK6 10783
Affinity Capture-MS Homo sapiens
19 ASF1B  
Affinity Capture-MS Homo sapiens
20 BCAS1  
Affinity Capture-MS Homo sapiens
21 SHANK2 22941
Affinity Capture-Western Homo sapiens
22 DLGAP1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
23 T3JAM  
Affinity Capture-MS Homo sapiens
24 Mta1  
Affinity Capture-MS Rattus norvegicus
25 NFKBIA  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
26 SNCG 6623
Affinity Capture-MS Homo sapiens
27 LOC499110  
Affinity Capture-MS Rattus norvegicus
28 Grin3a  
Affinity Capture-MS Rattus norvegicus
29 RGS2  
Two-hybrid Homo sapiens
30 BCL2L11  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
31 Pfkm  
Affinity Capture-MS Rattus norvegicus
32 MYO5A 4644
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
33 Cacnb1  
Affinity Capture-MS Rattus norvegicus
34 DLG4  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
35 TXNDC17 84817
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
36 NTRK2 4915
Affinity Capture-Western Homo sapiens
37 Ldha 24533
Affinity Capture-MS Rattus norvegicus
38 Mtr  
Affinity Capture-MS Rattus norvegicus
39 Dlgh4  
Affinity Capture-MS Rattus norvegicus
40 Glul 24957
Affinity Capture-MS Rattus norvegicus
41 DYNC1I2 1781
Affinity Capture-MS Homo sapiens
42 RAB4A 5867
Two-hybrid Homo sapiens
43 Dnm2 25751
Affinity Capture-MS Rattus norvegicus
44 Pard3  
Affinity Capture-MS Rattus norvegicus
45 DDX3X 1654
Affinity Capture-MS Homo sapiens
46 PABPC1 26986
Affinity Capture-MS Homo sapiens
47 Myo10  
Affinity Capture-MS Mus musculus
48 GPHN  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
49 DNAJB9 4189
Two-hybrid Homo sapiens
50 DLG2 1740
Affinity Capture-Western Homo sapiens
51 Mdh1 24551
Affinity Capture-MS Rattus norvegicus
52 PAK1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
53 Vim 81818
Affinity Capture-MS Rattus norvegicus
54 Aldoa 24189
Affinity Capture-MS Rattus norvegicus
55 Dync1h1 29489
Affinity Capture-MS Rattus norvegicus
56 Dnmt3a  
Affinity Capture-MS Mus musculus
57 Actb 81822
Affinity Capture-MS Rattus norvegicus
58 Glud1 24399
Affinity Capture-MS Rattus norvegicus
59 Gphn  
Affinity Capture-MS Rattus norvegicus
60 Nos1  
Affinity Capture-MS Rattus norvegicus
61 Hip2 predicted  
Affinity Capture-MS Rattus norvegicus
62 RGD1309427  
Affinity Capture-MS Rattus norvegicus
63 DYNLL1 8655
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
64 Pfkp 60416
Affinity Capture-MS Rattus norvegicus
65 N/A  
Affinity Capture-MS Rattus norvegicus
66 DNCI1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
67 NRF1 4899
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
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