Gene description for WDR34
Gene name WD repeat domain 34
Gene symbol WDR34
Other names/aliases DIC5
FAP133
SRTD11
bA216B9.3
Species Homo sapiens
 Database cross references - WDR34
ExoCarta ExoCarta_89891
Vesiclepedia VP_89891
Entrez Gene 89891
HGNC 28296
MIM 613363
UniProt Q96EX3  
 WDR34 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for WDR34
Molecular Function
    protein binding GO:0005515 IPI
    dynein light chain binding GO:0045503 IBA
    dynein light chain binding GO:0045503 IPI
    dynein heavy chain binding GO:0045504 IBA
Biological Process
    intraciliary retrograde transport GO:0035721 IMP
    intraciliary transport GO:0042073 IBA
    cilium assembly GO:0060271 IGI
Subcellular Localization
    cytoplasm GO:0005737 IDA
    centrosome GO:0005813 IDA
    centriole GO:0005814 ISS
    cytosol GO:0005829 IDA
    cytoplasmic dynein complex GO:0005868 IBA
    cytoplasmic dynein complex GO:0005868 IDA
    cilium GO:0005929 IDA
    cilium GO:0005929 TAS
    axoneme GO:0005930 ISS
    filopodium GO:0030175 IEA
    ciliary basal body GO:0036064 IDA
    ciliary basal body GO:0036064 ISS
    ciliary plasm GO:0097014 IBA
    ciliary tip GO:0097542 TAS
 Experiment description of studies that identified WDR34 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for WDR34
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TBC1D31  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 SUMF2 25870
Affinity Capture-MS Homo sapiens
3 MAP2K2 5605
Affinity Capture-MS Homo sapiens
4 FCGR3B  
Affinity Capture-MS Homo sapiens
5 H3F3A 3020
Affinity Capture-MS Homo sapiens
6 DYNC1I1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 CCT3 7203
Affinity Capture-MS Homo sapiens
8 COIL  
Two-hybrid Homo sapiens
9 DYNC2LI1 51626
Affinity Capture-MS Homo sapiens
10 DYNLT1 6993
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
11 XPO1 7514
Affinity Capture-MS Homo sapiens
12 CCT2 10576
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 DOK3 79930
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 KLK11 11012
Affinity Capture-MS Homo sapiens
15 TMEM25  
Affinity Capture-MS Homo sapiens
16 UBR4 23352
Affinity Capture-MS Homo sapiens
17 FAM96A  
Affinity Capture-MS Homo sapiens
18 DYNLRB1 83658
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 TCP1 6950
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 TRAF6 7189
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
21 UXT 8409
Affinity Capture-MS Homo sapiens
22 CCT4 10575
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 CCT6B 10693
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 CCNJL  
Affinity Capture-MS Homo sapiens
25 TAB3 257397
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
26 MAP3K7  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
27 Dynlrb1 67068
Affinity Capture-MS Mus musculus
28 TRIM54  
Two-hybrid Homo sapiens
29 MAPK6  
Two-hybrid Homo sapiens
30 TCTEX1D2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 MEOX2  
Two-hybrid Homo sapiens
32 CDK13 8621
Affinity Capture-MS Homo sapiens
33 SUSD4  
Affinity Capture-MS Homo sapiens
34 WDR60 55112
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 KSR2  
Affinity Capture-MS Homo sapiens
36 RBM14-RBM4 100526737
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 NSUN5P1  
Affinity Capture-MS Homo sapiens
38 TAB2  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
39 CCT6A 908
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 TCTEX1D4  
Affinity Capture-MS Homo sapiens
41 DUSP4 1846
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 SLURP1  
Affinity Capture-MS Homo sapiens
43 ARRDC3 57561
Affinity Capture-MS Homo sapiens
44 Dynll1 56455
Affinity Capture-MS Mus musculus
45 DYNLL2 140735
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 DNAI2  
Affinity Capture-MS Homo sapiens
47 DYNLRB2 83657
Affinity Capture-MS Homo sapiens
48 DYNLL1 8655
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which WDR34 is involved
PathwayEvidenceSource
Cilium Assembly TAS Reactome
Intraflagellar transport TAS Reactome
Organelle biogenesis and maintenance TAS Reactome





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