Gene description for DYNLT1
Gene name dynein, light chain, Tctex-type 1
Gene symbol DYNLT1
Other names/aliases CW-1
TCTEL1
tctex-1
Species Homo sapiens
 Database cross references - DYNLT1
ExoCarta ExoCarta_6993
Vesiclepedia VP_6993
Entrez Gene 6993
HGNC 11697
MIM 601554
UniProt P63172  
 DYNLT1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for DYNLT1
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    dynein intermediate chain binding GO:0045505 IBA
Biological Process
    establishment of mitotic spindle orientation GO:0000132 ISS
    microtubule-based movement GO:0007018 IBA
    nervous system development GO:0007399 IEA
    regulation of G protein-coupled receptor signaling pathway GO:0008277 ISS
    intracellular transport of viral protein in host cell GO:0019060 IMP
    symbiont entry into host cell GO:0046718 IEA
    negative regulation of neurogenesis GO:0050768 ISS
    cell division GO:0051301 IEA
    microtubule-dependent intracellular transport of viral material towards nucleus GO:0075521 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    cytoplasm GO:0005737 IBA
    Golgi apparatus GO:0005794 IEA
    spindle GO:0005819 IEA
    cytoplasmic dynein complex GO:0005868 IBA
    cytoplasmic dynein complex GO:0005868 IDA
    cytoplasmic dynein complex GO:0005868 ISS
    cytoplasmic microtubule GO:0005881 IDA
    dynein complex GO:0030286 IPI
    secretory granule lumen GO:0034774 TAS
    host cell GO:0043657 IEA
    secretory vesicle GO:0099503 IDA
    ficolin-1-rich granule lumen GO:1904813 TAS
 Experiment description of studies that identified DYNLT1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
EM
Biophysical techniques
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 363
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 364
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 365
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DYNLT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 NPEPPS 9520
Co-fractionation Homo sapiens
3 RIOK1 83732
Proximity Label-MS Homo sapiens
4 HSPB7  
Two-hybrid Homo sapiens
5 KLK15  
Two-hybrid Homo sapiens
6 CLIP1 6249
Affinity Capture-MS Homo sapiens
7 SNAP23 8773
Proximity Label-MS Homo sapiens
8 DYNC1I2 1781
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
9 YWHAE 7531
Affinity Capture-MS Homo sapiens
10 MTPAP 55149
Proximity Label-MS Homo sapiens
11 DYNC1I1  
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
12 MCCC1 56922
Proximity Label-MS Homo sapiens
13 XRN2 22803
Two-hybrid Homo sapiens
14 ANXA6 309
Proximity Label-MS Homo sapiens
15 APP 351
Reconstituted Complex Homo sapiens
16 BRWD1  
Proximity Label-MS Homo sapiens
17 ILF2 3608
Two-hybrid Homo sapiens
18 CAPZB 832
Affinity Capture-MS Homo sapiens
19 TCTEX1D2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Two-hybrid Homo sapiens
20 Dync1h1 13424
Affinity Capture-MS Mus musculus
21 DHX33  
Two-hybrid Homo sapiens
22 C11orf30  
Proximity Label-MS Homo sapiens
23 PC 5091
Proximity Label-MS Homo sapiens
24 DYNC1LI2 1783
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
25 YES1 7525
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
26 PITX2  
Two-hybrid Homo sapiens
27 KRI1  
Proximity Label-MS Homo sapiens
28 AMER1  
Proximity Label-MS Homo sapiens
29 PLD2 5338
Affinity Capture-MS Homo sapiens
30 DYNLL1 8655
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 TUBA3C 7278
Two-hybrid Homo sapiens
32 DDX41 51428
Proximity Label-MS Homo sapiens
33 NSFL1C 55968
Proximity Label-MS Homo sapiens
34 PSMA1 5682
Two-hybrid Homo sapiens
35 SDCBP 6386
Two-hybrid Homo sapiens
36 DSG2 1829
Proximity Label-MS Homo sapiens
37 SOD1 6647
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
38 SNX4 8723
Co-fractionation Homo sapiens
39 CPNE3 8895
Proximity Label-MS Homo sapiens
40 DYNLT1 6993
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
41 CLASP2 23122
Affinity Capture-MS Homo sapiens
42 BRPF1  
Proximity Label-MS Homo sapiens
43 LMNA 4000
Co-fractionation Homo sapiens
44 UXT 8409
Two-hybrid Homo sapiens
45 PELO 53918
Two-hybrid Homo sapiens
46 BTF3L4 91408
Proximity Label-MS Homo sapiens
47 C1orf35  
Proximity Label-MS Homo sapiens
48 LYN 4067
Proximity Label-MS Homo sapiens
49 MAPRE1 22919
Affinity Capture-MS Homo sapiens
50 ACACA 31
Proximity Label-MS Homo sapiens
51 ZCCHC17  
Proximity Label-MS Homo sapiens
52 C2orf44  
Proximity Label-MS Homo sapiens
53 PCCA 5095
Proximity Label-MS Homo sapiens
54 RPA2 6118
Co-fractionation Homo sapiens
55 WDYHV1  
Two-hybrid Homo sapiens
56 NBN 4683
Proximity Label-MS Homo sapiens
57 DNAJA1 3301
Affinity Capture-MS Homo sapiens
58 LUC7L2 51631
Proximity Label-MS Homo sapiens
59 HIST1H1C 3006
Proximity Label-MS Homo sapiens
60 DDX54 79039
Two-hybrid Homo sapiens
61 PTGES3L-AARSD1 100885850
Two-hybrid Homo sapiens
62 DBT 1629
Proximity Label-MS Homo sapiens
63 DYNLL2 140735
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 PHF12  
Proximity Label-MS Homo sapiens
65 DYNLRB2 83657
Affinity Capture-MS Homo sapiens
66 RAD21 5885
Two-hybrid Homo sapiens
67 DYNLT3 6990
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Two-hybrid Homo sapiens
Negative Genetic Homo sapiens
68 BAG3 9531
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
69 ECH1 1891
Proximity Label-MS Homo sapiens
70 NOC3L 64318
Proximity Label-MS Homo sapiens
71 RRAGA 10670
Two-hybrid Homo sapiens
72 TIPRL 261726
Affinity Capture-MS Homo sapiens
73 TPR 7175
Co-fractionation Homo sapiens
74 PTH2R  
Two-hybrid Homo sapiens
75 CKAP2  
Proximity Label-MS Homo sapiens
76 LMNB2 84823
Co-fractionation Homo sapiens
77 SIRPA 140885
Two-hybrid Homo sapiens
78 CP 1356
Proximity Label-MS Homo sapiens
79 CUL3 8452
Affinity Capture-MS Homo sapiens
80 YWHAZ 7534
Affinity Capture-MS Homo sapiens
81 TUBB4B 10383
Affinity Capture-MS Homo sapiens
82 LTBP4 8425
Proximity Label-MS Homo sapiens
83 FUS 2521
Proximity Label-MS Homo sapiens
84 DCTN1 1639
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
85 PRMT5 10419
Proximity Label-MS Homo sapiens
86 GNA11 2767
Proximity Label-MS Homo sapiens
87 WDR60 55112
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
88 FXR2 9513
Two-hybrid Homo sapiens
89 GNB2 2783
Proximity Label-MS Homo sapiens
90 RPL22 6146
Proximity Label-MS Homo sapiens
91 DDB1 1642
Proximity Label-MS Homo sapiens
92 PVR 5817
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
93 LYAR 55646
Proximity Label-MS Homo sapiens
94 TCEANC  
Two-hybrid Homo sapiens
95 OLFM3  
Two-hybrid Homo sapiens
96 KRT19 3880
Proximity Label-MS Homo sapiens
97 CPNE8 144402
Proximity Label-MS Homo sapiens
98 FTSJ3 117246
Proximity Label-MS Homo sapiens
99 GNL3 26354
Proximity Label-MS Homo sapiens
100 FARP1 10160
Proximity Label-MS Homo sapiens
101 NKAP 79576
Proximity Label-MS Homo sapiens
102 DYNC2LI1 51626
Affinity Capture-MS Homo sapiens
103 PAFAH1B1 5048
Affinity Capture-MS Homo sapiens
104 RBM14 10432
Two-hybrid Homo sapiens
105 KIF2A 3796
Two-hybrid Homo sapiens
106 WDR34 89891
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
107 CCDC68  
Two-hybrid Homo sapiens
108 PLEC 5339
Proximity Label-MS Homo sapiens
109 SPTBN2 6712
Proximity Label-MS Homo sapiens
110 ERH 2079
Proximity Label-MS Homo sapiens
111 Dync1li1 235661
Affinity Capture-MS Mus musculus
112 PPIG 9360
Proximity Label-MS Homo sapiens
113 RPA1 6117
Co-fractionation Homo sapiens
114 MAPRE2 10982
Affinity Capture-MS Homo sapiens
115 S100A10 6281
Proximity Label-MS Homo sapiens
116 NSA2  
Proximity Label-MS Homo sapiens
117 GNB1 2782
Proximity Label-MS Homo sapiens
118 KLHL7  
Proximity Label-MS Homo sapiens
119 ACOX2  
Two-hybrid Homo sapiens
120 AARSD1 80755
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
121 SSBP1 6742
Proximity Label-MS Homo sapiens
122 RAD50 10111
Proximity Label-MS Homo sapiens
123 RAVER1 125950
Co-fractionation Homo sapiens
124 DYNC1H1 1778
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
125 DDX27 55661
Proximity Label-MS Homo sapiens
126 TXN 7295
Two-hybrid Homo sapiens
127 CLASP1 23332
Affinity Capture-MS Homo sapiens
128 NOC2L 26155
Proximity Label-MS Homo sapiens
129 PRKCA 5578
Proximity Label-MS Homo sapiens
130 PCCB 5096
Proximity Label-MS Homo sapiens
131 DYNLRB1 83658
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
132 TCTEX1D4  
Affinity Capture-MS Homo sapiens
133 LTV1  
Proximity Label-MS Homo sapiens
134 SUMO3 6612
Co-fractionation Homo sapiens
135 NHSL2  
Two-hybrid Homo sapiens
136 ANXA2 302
Proximity Label-MS Homo sapiens
137 UBA6 55236
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
138 VIM 7431
Affinity Capture-MS Homo sapiens
139 VPS33B 26276
Proximity Label-MS Homo sapiens
140 PRMT1 3276
Affinity Capture-MS Homo sapiens
141 DYNC1LI1 51143
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
142 GNAI3 2773
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DYNLT1 is involved
PathwayEvidenceSource
Immune System TAS Reactome
Innate Immune System TAS Reactome
Neutrophil degranulation TAS Reactome





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