Gene description for RAD50
Gene name RAD50 homolog (S. cerevisiae)
Gene symbol RAD50
Other names/aliases NBSLD
RAD502
hRad50
Species Homo sapiens
 Database cross references - RAD50
ExoCarta ExoCarta_10111
Entrez Gene 10111
HGNC 9816
MIM 604040
UniProt Q92878  
 RAD50 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for RAD50
Molecular Function
    ATP binding GO:0005524 IEA
    single-stranded DNA endodeoxyribonuclease activity GO:0000014 IDA
    metal ion binding GO:0046872 IEA
    3'-5' exonuclease activity GO:0008408 IDA
    DNA binding GO:0003677 IDA
    protein binding, bridging GO:0030674 IDA
    protein binding GO:0005515 IPI
    ATP-dependent DNA helicase activity GO:0004003 IMP
Biological Process
    positive regulation of kinase activity GO:0033674 IDA
    regulation of mitotic recombination GO:0000019 IDA
    DNA duplex unwinding GO:0032508 IMP
    telomere maintenance GO:0000723 TAS
    DNA recombination GO:0006310 IDA
    double-strand break repair via homologous recombination GO:0000724 TAS
    viral process GO:0016032 IEA
    double-strand break repair GO:0006302 TAS
    DNA repair GO:0006281 TAS
    cellular response to DNA damage stimulus GO:0006974 IDA
    positive regulation of protein autophosphorylation GO:0031954 IDA
    telomere maintenance via telomerase GO:0007004 IDA
    reciprocal meiotic recombination GO:0007131 TAS
    nucleic acid phosphodiester bond hydrolysis GO:0090305 IDA
Subcellular Localization
    membrane GO:0016020 IDA
    nucleoplasm GO:0005654 TAS
    site of double-strand break GO:0035861 IDA
    nuclear chromosome, telomeric region GO:0000784 IDA
    pronucleus GO:0045120 IEA
    Mre11 complex GO:0030870 IDA
 Experiment description of studies that identified RAD50 in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RAD50
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BLM 641
Co-purification Homo sapiens
2 RFC1 5981
Co-purification Homo sapiens
3 TERF2IP 54386
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
4 HNF4A  
Affinity Capture-MS Homo sapiens
5 BRCA1 672
Co-purification Homo sapiens
Co-purification Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
6 TERF2  
Affinity Capture-Western Homo sapiens
7 MRE11A 4361
Invivo Homo sapiens
Invitro Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
8 ATM  
Co-purification Homo sapiens
9 RINT-1  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
10 NBS1  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
11 MDC1  
Affinity Capture-Western Homo sapiens
12 MSH6 2956
Co-purification Homo sapiens
13 MSH2 4436
Co-purification Homo sapiens
14 H2AFX 3014
Co-localization Homo sapiens
15 MLH1 4292
Co-purification Homo sapiens
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