Gene description for NCK1
Gene name NCK adaptor protein 1
Gene symbol NCK1
Other names/aliases NCK
NCKalpha
nck-1
Species Homo sapiens
 Database cross references - NCK1
ExoCarta ExoCarta_4690
Vesiclepedia VP_4690
Entrez Gene 4690
HGNC 7664
MIM 600508
UniProt P16333  
 NCK1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for NCK1
Molecular Function
    protein kinase inhibitor activity GO:0004860 IDA
    signaling receptor binding GO:0005102 IPI
    protein binding GO:0005515 IPI
    cytoskeletal anchor activity GO:0008093 NAS
    protein domain specific binding GO:0019904 IEA
    signaling receptor complex adaptor activity GO:0030159 NAS
    protein-macromolecule adaptor activity GO:0030674 IDA
    receptor tyrosine kinase binding GO:0030971 IBA
    receptor tyrosine kinase binding GO:0030971 IPI
    signaling adaptor activity GO:0035591 IBA
    signaling adaptor activity GO:0035591 IDA
    cadherin binding GO:0045296 HDA
    ephrin receptor binding GO:0046875 IBA
    eukaryotic initiation factor eIF2 binding GO:0071074 IPI
    protein sequestering activity GO:0140311 IDA
    molecular condensate scaffold activity GO:0140693 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    substrate-dependent cell migration, cell extension GO:0006930 IEA
    actin filament organization GO:0007015 IEA
    signal complex assembly GO:0007172 NAS
    positive regulation of neuron projection development GO:0010976 IEA
    cell migration GO:0016477 IBA
    lamellipodium assembly GO:0030032 IEA
    regulation of cell migration GO:0030334 IEA
    positive regulation of actin filament polymerization GO:0030838 IMP
    negative regulation of peptidyl-serine phosphorylation GO:0033137 IDA
    response to endoplasmic reticulum stress GO:0034976 IDA
    positive regulation of translation in response to endoplasmic reticulum stress GO:0036493 IBA
    positive regulation of translation in response to endoplasmic reticulum stress GO:0036493 IDA
    positive regulation of T cell proliferation GO:0042102 IMP
    T cell activation GO:0042110 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    negative regulation of insulin receptor signaling pathway GO:0046627 IMP
    ephrin receptor signaling pathway GO:0048013 IBA
    negative regulation of T cell receptor signaling pathway GO:0050860 IDA
    antiviral innate immune response GO:0140374 IDA
    positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway GO:1902237 IBA
    positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway GO:1902237 IDA
    positive regulation of cap-dependent translational initiation GO:1903676 IDA
    positive regulation of cap-independent translational initiation GO:1903679 IDA
    negative regulation of PERK-mediated unfolded protein response GO:1903898 IBA
    negative regulation of PERK-mediated unfolded protein response GO:1903898 IDA
    negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation GO:1903912 IDA
Subcellular Localization
    protein phosphatase type 1 complex GO:0000164 IDA
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 NAS
    endoplasmic reticulum GO:0005783 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    ribosome GO:0005840 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 IEA
    vesicle membrane GO:0012506 IEA
 Experiment description of studies that identified NCK1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NCK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 ARHGAP21 57584
Affinity Capture-MS Homo sapiens
3 DLG5 9231
Affinity Capture-MS Homo sapiens
4 ABL2  
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
5 WIPF2 147179
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 TRRAP 8295
Affinity Capture-MS Homo sapiens
7 DAG1 1605
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
8 WASF2 10163
Affinity Capture-MS Homo sapiens
9 FMR1 2332
Co-fractionation Homo sapiens
10 GIT2 9815
Affinity Capture-MS Homo sapiens
11 SOS1 6654
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
12 PAK2 5062
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
13 ADGRL2 23266
Two-hybrid Homo sapiens
14 SOCS1  
Reconstituted Complex Homo sapiens
15 LAD1 3898
Affinity Capture-MS Homo sapiens
16 WASF1 8936
Affinity Capture-MS Homo sapiens
17 DOK2 9046
Affinity Capture-Western Homo sapiens
18 RALGPS1  
Affinity Capture-Western Homo sapiens
19 MET 4233
Reconstituted Complex Homo sapiens
20 DUSP12 11266
Affinity Capture-MS Homo sapiens
21 TOP2A 7153
Affinity Capture-MS Homo sapiens
22 DROSHA  
Affinity Capture-MS Homo sapiens
23 HEATR3 55027
Affinity Capture-MS Homo sapiens
24 SEMA6A  
Affinity Capture-MS Homo sapiens
25 KIF22  
Affinity Capture-MS Homo sapiens
26 APBB2  
Affinity Capture-MS Homo sapiens
27 SMARCA1 6594
Affinity Capture-MS Homo sapiens
28 HIST2H3A 333932
Affinity Capture-MS Homo sapiens
29 TNIK 23043
Affinity Capture-MS Homo sapiens
30 KHDRBS1 10657
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
31 SYK 6850
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
32 ABI1 10006
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
33 CD3E 916
Reconstituted Complex Homo sapiens
34 KDR  
Affinity Capture-Western Homo sapiens
35 WASL 8976
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 Mbp 17196
Protein-peptide Mus musculus
37 DNM1 1759
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
38 PKN2 5586
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
39 BRK1 55845
Affinity Capture-MS Homo sapiens
40 CHN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 ERBB2 2064
Reconstituted Complex Homo sapiens
42 FGFR1 2260
Protein-peptide Homo sapiens
43 CBL 867
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
44 HIST3H2BB 128312
Affinity Capture-MS Homo sapiens
45 PAK1 5058
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
46 WIPF1 7456
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 PFN1 5216
Reconstituted Complex Homo sapiens
48 UBR4 23352
Affinity Capture-MS Homo sapiens
49 NEDD9 4739
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
50 BCAR1 9564
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
51 KIT 3815
Reconstituted Complex Homo sapiens
52 FASLG 356
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
53 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
54 UXT 8409
Affinity Capture-MS Homo sapiens
55 NCKIPSD 51517
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
56 Mta3  
Reconstituted Complex Mus musculus
57 WHSC1 7468
Affinity Capture-MS Homo sapiens
58 PDGFRB 5159
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
59 ERBB3 2065
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
60 SOCS7  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
61 TUBA1A 7846
Affinity Capture-MS Homo sapiens
62 PRPS2 5634
Affinity Capture-MS Homo sapiens
63 HIST1H2AJ 8331
Affinity Capture-MS Homo sapiens
64 TRIM14 9830
Affinity Capture-MS Homo sapiens
65 UBASH3B 84959
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
66 RPS27A 6233
Affinity Capture-MS Homo sapiens
67 CDH1 999
Proximity Label-MS Homo sapiens
68 GIT1 28964
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 ARHGEF6 9459
Affinity Capture-MS Homo sapiens
70 PIK3R1 5295
Affinity Capture-Western Homo sapiens
71 RTEL1 51750
Affinity Capture-MS Homo sapiens
72 LRFN4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 NUMA1 4926
Affinity Capture-MS Homo sapiens
74 MINK1 50488
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
75 ARHGEF7 8874
Affinity Capture-MS Homo sapiens
76 ARHGAP32  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
77 PHIP 55023
Affinity Capture-MS Homo sapiens
78 CLINT1 9685
Affinity Capture-MS Homo sapiens
79 GAB2 9846
Reconstituted Complex Homo sapiens
80 BAZ1B 9031
Affinity Capture-MS Homo sapiens
81 CHN2  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
82 NCKAP1 10787
Affinity Capture-MS Homo sapiens
83 CAV2 858
Reconstituted Complex Homo sapiens
84 PIK3AP1  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
85 ERBB4 2066
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
86 SMARCA5 8467
Affinity Capture-MS Homo sapiens
87 EGFR 1956
Protein-peptide Homo sapiens
Two-hybrid Homo sapiens
PCA Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
88 NHSL1  
Affinity Capture-MS Homo sapiens
89 Dok1  
Affinity Capture-Western Mus musculus
90 PTK2 5747
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
91 RAD54L 8438
Affinity Capture-MS Homo sapiens
92 CYFIP2 26999
Affinity Capture-MS Homo sapiens
93 NCKAP5  
Affinity Capture-MS Homo sapiens
94 GAB1  
Reconstituted Complex Homo sapiens
95 H1F0 3005
Affinity Capture-MS Homo sapiens
96 RRAS 6237
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
97 ADIPOR2 79602
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 Caskin1  
Two-hybrid Rattus norvegicus
99 DCC  
Affinity Capture-Western Homo sapiens
100 DLL1  
Affinity Capture-MS Homo sapiens
101 MAP4K4 9448
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
102 CPNE5 57699
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 INPP5D 3635
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
104 FAM217B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 BAIAP2 10458
Affinity Capture-MS Homo sapiens
106 CASKIN2  
Affinity Capture-MS Homo sapiens
107 KIAA1522 57648
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 ARAP1 116985
Affinity Capture-MS Homo sapiens
109 RASA1 5921
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
110 BLNK  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
111 TNK2 10188
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 SH3PXD2B 285590
Affinity Capture-MS Homo sapiens
113 PARP1 142
Affinity Capture-MS Homo sapiens
114 SHANK3 85358
Two-hybrid Homo sapiens
115 WIPF3  
Affinity Capture-MS Homo sapiens
116 GRB2 2885
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
117 TTC28 23331
Affinity Capture-MS Homo sapiens
118 H1FX 8971
Affinity Capture-MS Homo sapiens
119 HIST2H2AA3 8337
Affinity Capture-MS Homo sapiens
120 UBA52 7311
Two-hybrid Homo sapiens
121 EPHB1 2047
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
122 ABL1 25
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
123 HIST1H2BD 3017
Affinity Capture-MS Homo sapiens
124 FAM83H 286077
Affinity Capture-MS Homo sapiens
125 ADAM15 8751
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
126 WAS 7454
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
127 NCK2 8440
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
Affinity Capture-MS Homo sapiens
128 Mta1  
Reconstituted Complex Mus musculus
129 TBK1 29110
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
130 DOK1 1796
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
131 ABI2 10152
Affinity Capture-MS Homo sapiens
132 H2AFZ 3015
Affinity Capture-MS Homo sapiens
133 SYNPO 11346
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
134 PAPSS1 9061
Affinity Capture-MS Homo sapiens
135 PXN 5829
Reconstituted Complex Homo sapiens
136 LCP2 3937
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
137 RAD50 10111
Affinity Capture-MS Homo sapiens
138 SOS2 6655
Affinity Capture-MS Homo sapiens
139 GHR  
Co-localization Homo sapiens
140 DRD4  
Reconstituted Complex Homo sapiens
141 CYFIP1 23191
Affinity Capture-MS Homo sapiens
142 NHSL2  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
143 PHC2  
Two-hybrid Homo sapiens
144 HIST1H1D 3007
Affinity Capture-MS Homo sapiens
145 ASAP2 8853
Affinity Capture-MS Homo sapiens
146 MAP4K1  
Two-hybrid Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which NCK1 is involved
PathwayEvidenceSource
Activation of RAC1 IEA Reactome
Adaptive Immune System TAS Reactome
Adaptive Immune System IEA Reactome
Antigen activates B Cell Receptor (BCR) leading to generation of second messengers TAS Reactome
Antigen activates B Cell Receptor (BCR) leading to generation of second messengers IEA Reactome
Antiviral mechanism by IFN-stimulated genes TAS Reactome
Axon guidance IEA Reactome
Axon guidance TAS Reactome
Cell-Cell communication TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
DCC mediated attractive signaling TAS Reactome
DCC mediated attractive signaling IEA Reactome
Developmental Biology IEA Reactome
Developmental Biology TAS Reactome
Disease TAS Reactome
Disease IEA Reactome
Downstream signal transduction TAS Reactome
Fcgamma receptor (FCGR) dependent phagocytosis TAS Reactome
Fcgamma receptor (FCGR) dependent phagocytosis IEA Reactome
FCGR3A-mediated phagocytosis TAS Reactome
FCGR3A-mediated phagocytosis IEA Reactome
Generation of second messenger molecules TAS Reactome
Immune System TAS Reactome
Immune System IEA Reactome
Infectious disease TAS Reactome
Infectious disease IEA Reactome
Innate Immune System TAS Reactome
Innate Immune System IEA Reactome
Interferon Signaling TAS Reactome
Leishmania infection TAS Reactome
Leishmania infection IEA Reactome
Leishmania phagocytosis TAS Reactome
Leishmania phagocytosis IEA Reactome
Nephrin family interactions TAS Reactome
Nervous system development IEA Reactome
Nervous system development TAS Reactome
Netrin-1 signaling TAS Reactome
Netrin-1 signaling IEA Reactome
Parasite infection TAS Reactome
Parasite infection IEA Reactome
Parasitic Infection Pathways TAS Reactome
Parasitic Infection Pathways IEA Reactome
PKR-mediated signaling TAS Reactome
Potential therapeutics for SARS TAS Reactome
Regulation of actin dynamics for phagocytic cup formation TAS Reactome
Regulation of actin dynamics for phagocytic cup formation IEA Reactome
RHO GTPase cycle TAS Reactome
RHO GTPase Effectors TAS Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate WASPs and WAVEs TAS Reactome
RHO GTPases Activate WASPs and WAVEs IEA Reactome
RHOU GTPase cycle TAS Reactome
RHOV GTPase cycle TAS Reactome
SARS-CoV Infections TAS Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by PDGF TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by ROBO receptors IEA Reactome
Signaling by the B Cell Receptor (BCR) TAS Reactome
Signaling by the B Cell Receptor (BCR) IEA Reactome
Signaling by VEGF TAS Reactome
TCR signaling TAS Reactome
VEGFA-VEGFR2 Pathway TAS Reactome
Viral Infection Pathways TAS Reactome





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