Gene description for NCK1
Gene name NCK adaptor protein 1
Gene symbol NCK1
Other names/aliases NCK
NCKalpha
nck-1
Species Homo sapiens
 Database cross references - NCK1
ExoCarta ExoCarta_4690
Vesiclepedia VP_4690
Entrez Gene 4690
HGNC 7664
MIM 600508
UniProt P16333  
 NCK1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for NCK1
Molecular Function
    protein binding, bridging GO:0030674 IC
    cytoskeletal adaptor activity GO:0008093 NAS
    protein binding GO:0005515 IPI
    receptor binding GO:0005102 IPI
    SH3/SH2 adaptor activity GO:0005070 NAS
    protein kinase inhibitor activity GO:0004860 IDA
    protein domain specific binding GO:0019904 IEA
    receptor signaling complex scaffold activity GO:0030159 NAS
    receptor tyrosine kinase binding GO:0030971 IPI
    eukaryotic initiation factor eIF2 binding GO:0071074 IPI
Biological Process
    positive regulation of T cell proliferation GO:0042102 IMP
    negative regulation of PERK-mediated unfolded protein response GO:1903898 IDA
    axon guidance GO:0007411 TAS
    signal complex assembly GO:0007172 NAS
    negative regulation of protein kinase activity GO:0006469 IDA
    negative regulation of cell death GO:0060548 IDA
    T cell activation GO:0042110 IMP
    small GTPase mediated signal transduction GO:0007264 TAS
    substrate-dependent cell migration, cell extension GO:0006930 IEA
    response to other organism GO:0051707 IEA
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    actin filament organization GO:0007015 IEA
    positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway GO:1902237 IDA
    negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation GO:1903912 IDA
    T cell receptor signaling pathway GO:0050852 TAS
    positive regulation of translation in response to endoplasmic reticulum stress GO:0036493 IDA
    negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress GO:1990441 IDA
    innate immune response GO:0045087 TAS
    positive regulation of actin filament polymerization GO:0030838 IMP
    peptidyl-serine dephosphorylation GO:0070262 IDA
    negative regulation of peptidyl-serine phosphorylation GO:0033137 IDA
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    positive regulation of cap-independent translational initiation GO:1903679 IDA
    positive regulation of cap-dependent translational initiation GO:1903676 IDA
    lamellipodium assembly GO:0030032 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
Subcellular Localization
    cell-cell junction GO:0005911 IEA
    vesicle membrane GO:0012506 IEA
    protein phosphatase type 1 complex GO:0000164 IDA
    nucleus GO:0005634 IEA
    ribosome GO:0005840 IDA
    endoplasmic reticulum GO:0005783 IDA
    cytoplasm GO:0005737 NAS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified NCK1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70|FLOT1
EV Enriched markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70|FLOT1
EV Enriched markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70|FLOT1
EV Enriched markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 363
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 364
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 365
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 231
MISEV standards
✘ EV Biophysical techniques
✔
Alix|CD63|CD9
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 232
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 233
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NCK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RASA1 5921
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
2 TNK2 10188
Affinity Capture-MS Homo sapiens
3 DOK2 9046
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
4 DNM1 1759
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
5 PKN2 5586
Reconstituted Complex Homo sapiens
6 CBLB 868
Affinity Capture-MS Homo sapiens
7 PXN  
Reconstituted Complex Homo sapiens
8 EGFR 1956
Reconstituted Complex Homo sapiens
9 RICS  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
10 PAK1 5058
Invitro Homo sapiens
11 WIPF1 7456
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
12 EPHB1 2047
Two-hybrid Homo sapiens
13 NCKAP1 10787
Two-hybrid Homo sapiens
14 CSNK1G2 1455
Affinity Capture-MS Homo sapiens
15 CAV2 858
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
16 EIF2B2 8892
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
17 ABL1 25
Invivo Homo sapiens
18 BCAR1 9564
Invivo Homo sapiens
19 FASLG 356
Invivo Homo sapiens
20 DAG1 1605
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
21 ERBB4 2066
Affinity Capture-MS Homo sapiens
22 SOS1 6654
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
23 MAP4K4 9448
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
24 NCKIPSD 51517
Two-hybrid Homo sapiens
25 Mta3  
Reconstituted Complex Mus musculus
26 MINK1 50488
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
27 PTK2 5747
Reconstituted Complex Homo sapiens
28 PDGFRB 5159
Affinity Capture-MS Homo sapiens
29 EIF2S2 8894
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
30 SOCS7  
Invitro Homo sapiens
31 Mta1  
Reconstituted Complex Mus musculus
32 TBK1 29110
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
33 WAS 7454
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
34 RRAS 6237
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
35 NEDD9 4739
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
36 LCP2 3937
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
37 DCC  
Affinity Capture-Western Homo sapiens
38 DRD4  
Invivo Homo sapiens
39 KHDRBS1 10657
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
40 BLNK  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
41 RAC1 5879
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
42 ABI1 10006
Two-hybrid Homo sapiens
43 NTRK2 4915
Two-hybrid Homo sapiens
44 CRK 1398
Reconstituted Complex Homo sapiens
45 SOCS1  
Reconstituted Complex Homo sapiens
46 MAP4K1  
Two-hybrid Homo sapiens
47 WASL 8976
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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