Gene description for ERBB2
Gene name erb-b2 receptor tyrosine kinase 2
Gene symbol ERBB2
Other names/aliases CD340
HER-2
HER-2/neu
HER2
MLN 19
NEU
NGL
TKR1
Species Homo sapiens
 Database cross references - ERBB2
ExoCarta ExoCarta_2064
Vesiclepedia VP_2064
Entrez Gene 2064
HGNC 3430
MIM 164870
UniProt P04626  
 ERBB2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 16302729    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
Endothelial cells 26027894    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Malignant ascites 12147373    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for ERBB2
Molecular Function
    RNA polymerase I core binding GO:0001042 IDA
    protein tyrosine kinase activity GO:0004713 IDA
    protein tyrosine kinase activity GO:0004713 TAS
    transmembrane receptor protein tyrosine kinase activity GO:0004714 IBA
    transmembrane receptor protein tyrosine kinase activity GO:0004714 IDA
    transmembrane signaling receptor activity GO:0004888 IDA
    GPI-linked ephrin receptor activity GO:0005004 IEA
    transmembrane-ephrin receptor activity GO:0005005 IEA
    epidermal growth factor receptor activity GO:0005006 IEA
    fibroblast growth factor receptor activity GO:0005007 IEA
    hepatocyte growth factor receptor activity GO:0005008 IEA
    insulin receptor activity GO:0005009 IEA
    insulin-like growth factor receptor activity GO:0005010 IEA
    macrophage colony-stimulating factor receptor activity GO:0005011 IEA
    platelet-derived growth factor alpha-receptor activity GO:0005018 IEA
    platelet-derived growth factor beta-receptor activity GO:0005019 IEA
    stem cell factor receptor activity GO:0005020 IEA
    vascular endothelial growth factor receptor activity GO:0005021 IEA
    signaling receptor binding GO:0005102 IPI
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    boss receptor activity GO:0008288 IEA
    coreceptor activity GO:0015026 ISS
    growth factor binding GO:0019838 IDA
    receptor tyrosine kinase binding GO:0030971 IPI
    histone H3Y41 kinase activity GO:0035401 IEA
    placental growth factor receptor activity GO:0036332 IEA
    protein tyrosine kinase collagen receptor activity GO:0038062 IEA
    identical protein binding GO:0042802 IPI
    ErbB-3 class receptor binding GO:0043125 IPI
    protein heterodimerization activity GO:0046982 IPI
    brain-derived neurotrophic factor receptor activity GO:0060175 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    positive regulation of protein phosphorylation GO:0001934 ISS
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 TAS
    signal transduction GO:0007165 IDA
    cell surface receptor signaling pathway GO:0007166 IDA
    enzyme-linked receptor protein signaling pathway GO:0007167 TAS
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 IDA
    multicellular organism development GO:0007275 IBA
    heart development GO:0007507 IEA
    neuromuscular junction development GO:0007528 IEA
    motor neuron axon guidance GO:0008045 IEA
    cell population proliferation GO:0008283 IDA
    positive regulation of cell population proliferation GO:0008284 IBA
    insulin receptor signaling pathway GO:0008286 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 IEA
    Schwann cell development GO:0014044 IEA
    peptidyl-tyrosine phosphorylation GO:0018108 IDA
    neurogenesis GO:0022008 IBA
    neuron differentiation GO:0030182 IBA
    positive regulation of cell growth GO:0030307 IMP
    brain-derived neurotrophic factor receptor signaling pathway GO:0031547 IEA
    regulation of microtubule-based process GO:0032886 IDA
    immature T cell proliferation in thymus GO:0033080 IEA
    negative regulation of immature T cell proliferation in thymus GO:0033088 IEA
    positive regulation of Rho protein signal transduction GO:0035025 ISS
    intracellular signal transduction GO:0035556 IDA
    platelet-derived growth factor receptor-alpha signaling pathway GO:0035790 IEA
    platelet-derived growth factor receptor-beta signaling pathway GO:0035791 IEA
    vascular endothelial growth factor receptor-1 signaling pathway GO:0036323 IEA
    collagen-activated tyrosine kinase receptor signaling pathway GO:0038063 IEA
    vascular endothelial growth factor signaling pathway GO:0038084 IEA
    Kit signaling pathway GO:0038109 IEA
    ERBB2-ERBB3 signaling pathway GO:0038133 IEA
    ERBB2-EGFR signaling pathway GO:0038134 IDA
    ERBB2-ERBB4 signaling pathway GO:0038135 IEA
    macrophage colony-stimulating factor signaling pathway GO:0038145 IEA
    wound healing GO:0042060 IDA
    myelination GO:0042552 IEA
    negative regulation of apoptotic process GO:0043066 IBA
    positive regulation of MAP kinase activity GO:0043406 IDA
    positive regulation of MAPK cascade GO:0043410 IBA
    phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0043491 IDA
    positive regulation of translation GO:0045727 IMP
    regulation of angiogenesis GO:0045765 NAS
    positive regulation of cell adhesion GO:0045785 IDA
    positive regulation of transcription by RNA polymerase I GO:0045943 IMP
    insulin-like growth factor receptor signaling pathway GO:0048009 IEA
    hepatocyte growth factor receptor signaling pathway GO:0048012 IEA
    ephrin receptor signaling pathway GO:0048013 IEA
    oligodendrocyte differentiation GO:0048709 IEA
    positive regulation of epithelial cell proliferation GO:0050679 IDA
    regulation of ERK1 and ERK2 cascade GO:0070372 IMP
    cellular response to growth factor stimulus GO:0071363 IDA
    cellular response to epidermal growth factor stimulus GO:0071364 IMP
    semaphorin-plexin signaling pathway GO:0071526 ISS
    positive regulation of protein targeting to membrane GO:0090314 IDA
    neurotransmitter receptor localization to postsynaptic specialization membrane GO:0099645 IEA
Subcellular Localization
    semaphorin receptor complex GO:0002116 ISS
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    early endosome GO:0005769 IEA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 NAS
    plasma membrane GO:0005886 TAS
    basal plasma membrane GO:0009925 IBA
    endosome membrane GO:0010008 IDA
    membrane GO:0016020 NAS
    basolateral plasma membrane GO:0016323 IDA
    apical plasma membrane GO:0016324 IEA
    neuromuscular junction GO:0031594 IEA
    ruffle membrane GO:0032587 IEA
    ERBB3:ERBB2 complex GO:0038143 IDA
    presynaptic membrane GO:0042734 IEA
    myelin sheath GO:0043209 IEA
    receptor complex GO:0043235 IBA
    receptor complex GO:0043235 IDA
    receptor complex GO:0043235 TAS
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified ERBB2 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 19
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
FACS
PubMed ID 16302729    
Organism Homo sapiens
Experiment description "Purification, characterization and biological significance of tumor-derived exosomes."
Authors "Koga K, Matsumoto K, Akiyoshi T, Kubo M, Yamanaka N, Tasaki A, Nakashima H, Nakamura M, Kuroki S, Tanaka M, Katano M"
Journal name ACR
Publication year 2005
Sample Breast cancer cells
Sample name BT-474
MDA-MB-231
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Filtration
Immunobeads(HER2)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
FACS
6
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 1203
MISEV standards
EM
Biophysical techniques
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
10
Experiment ID 226
MISEV standards
EM
Biophysical techniques
GAPDH|CD9|FLOT1
Enriched markers
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
11
Experiment ID 363
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 364
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 365
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 56
MISEV standards
EM
Biophysical techniques
HSC70|MHCI|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 12147373    
Organism Homo sapiens
Experiment description Malignant effusions and immunogenic tumour-derived exosomes.
Authors "Andre F, Schartz NE, Movassagh M, Flament C, Pautier P, Morice P, Pomel C, Lhomme C, Escudier B, Le Chevalier T, Tursz T, Amigorena S, Raposo G, Angevin E, Zitvogel L"
Journal name LANCET
Publication year 2002
Sample Malignant ascites
Sample name Malignant ascites
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
15
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
16
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
17
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 191
MISEV standards
Biophysical techniques
Alix|CD81|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ERBB2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ITGB1 3688
Affinity Capture-Western Homo sapiens
2 TNS1 7145
Reconstituted Complex Homo sapiens
3 GRAP2 9402
Protein-peptide Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
4 ABL2  
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
5 AP2B1 163
Affinity Capture-Western Homo sapiens
6 TICAM2  
Affinity Capture-MS Homo sapiens
7 OCLN 100506658
Proximity Label-MS Homo sapiens
8 SHC1 6464
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
9 MATK  
Protein-peptide Homo sapiens
10 SORL1 6653
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
11 FRS3 10817
Affinity Capture-Western Homo sapiens
12 GJA1 2697
Proximity Label-MS Homo sapiens
13 HCST  
Affinity Capture-MS Homo sapiens
14 FLT4  
PCA Homo sapiens
15 LPAR6  
Affinity Capture-MS Homo sapiens
16 EPHB1 2047
PCA Homo sapiens
17 CRK 1398
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
18 KPNB1 3837
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
19 SHC4  
Reconstituted Complex Homo sapiens
20 HRAS 3265
Reconstituted Complex Homo sapiens
21 DAB1 1600
Protein-peptide Homo sapiens
22 SOCS1  
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
23 HSP90AB1 3326
Affinity Capture-Western Homo sapiens
24 SOCS3 9021
Reconstituted Complex Homo sapiens
25 CHEK2  
Negative Genetic Homo sapiens
26 GRB10 2887
Reconstituted Complex Homo sapiens
27 RET 5979
PCA Homo sapiens
28 APBB1  
Protein-peptide Homo sapiens
29 ANKS1A 23294
Protein-peptide Homo sapiens
30 TMEM200A 114801
Affinity Capture-MS Homo sapiens
31 SHD  
Reconstituted Complex Homo sapiens
32 FGFR3 2261
PCA Homo sapiens
33 GRB7 2886
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
34 FGFR1 2260
PCA Homo sapiens
35 SH2D5  
Protein-peptide Homo sapiens
36 IL6ST 3572
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
37 HPSE 10855
Affinity Capture-MS Homo sapiens
38 RDX 5962
Co-localization Homo sapiens
39 PLCG2 5336
Co-localization Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
40 HCK 3055
Reconstituted Complex Homo sapiens
41 NAALADL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 INSRR 3645
Affinity Capture-MS Homo sapiens
PCA Homo sapiens
43 PC 5091
Affinity Capture-MS Homo sapiens
44 PDGFRB 5159
Affinity Capture-Western Homo sapiens
PCA Homo sapiens
45 SOCS6  
Reconstituted Complex Homo sapiens
46 TLN1 7094
Protein-peptide Homo sapiens
47 FES 2242
Reconstituted Complex Homo sapiens
48 CDH2 1000
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
49 DUSP14 11072
Two-hybrid Homo sapiens
50 FBXW7  
Affinity Capture-MS Homo sapiens
51 MUC1 4582
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
52 SRC 6714
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Negative Genetic Homo sapiens
Reconstituted Complex Homo sapiens
53 STAT3 6774
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
54 MST1R 4486
PCA Homo sapiens
55 ARL15 54622
Affinity Capture-MS Homo sapiens
56 FER 2241
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
57 SHC3  
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
58 PIGH  
Affinity Capture-MS Homo sapiens
59 PLCG1 5335
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
60 SLITRK3  
Affinity Capture-MS Homo sapiens
61 EPHA1 2041
PCA Homo sapiens
62 ALK 238
PCA Homo sapiens
63 PPM1A 5494
Two-hybrid Homo sapiens
64 STX6 10228
Proximity Label-MS Homo sapiens
65 APEX1 328
Affinity Capture-RNA Homo sapiens
66 PTK7 5754
PCA Homo sapiens
67 EEF1G 1937
Affinity Capture-MS Homo sapiens
68 L1CAM 3897
Affinity Capture-Western Homo sapiens
69 SYK 6850
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
70 MAPK8IP1 9479
Protein-peptide Homo sapiens
71 USP2  
Biochemical Activity Homo sapiens
72 PTPRU  
Two-hybrid Homo sapiens
73 RAB35 11021
Proximity Label-MS Homo sapiens
74 SH3BGRL 6451
Affinity Capture-MS Homo sapiens
75 ROR2 4920
PCA Homo sapiens
76 CHN1  
Protein-peptide Homo sapiens
77 STYX  
Two-hybrid Homo sapiens
78 ERBB2 2064
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
PCA Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
PCA Homo sapiens
79 BCAR3 8412
Protein-peptide Homo sapiens
80 MCAM 4162
Proximity Label-MS Homo sapiens
81 TFRC 7037
Affinity Capture-MS Homo sapiens
82 MAS1  
Affinity Capture-MS Homo sapiens
83 YES1 7525
Reconstituted Complex Homo sapiens
84 TEK  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
85 EEA1 8411