ExoCarta: Gene summary

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Gene description for FGFR4
Gene name	fibroblast growth factor receptor 4
Gene symbol	FGFR4
Other names/aliases	CD334 JTK2 TKF
Species	Homo sapiens
Database cross references - FGFR4
ExoCarta	ExoCarta_2264
Vesiclepedia	VP_2264
Entrez Gene	2264
HGNC	3691
MIM	134935
UniProt	P22455
FGFR4 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells	34887515
Neuroblastoma cells	25944692

Gene ontology annotations for FGFR4

Molecular Function
	heparin binding	GO:0008201	IDA
	fibroblast growth factor binding	GO:0017134	IPI
	fibroblast growth factor-activated receptor activity	GO:0005007	IGI
	protein tyrosine kinase activity	GO:0004713	TAS
	protein binding	GO:0005515	IPI
	ATP binding	GO:0005524	IEA
Biological Process
	response to bile acid	GO:1903412	IMP
	peptidyl-tyrosine phosphorylation	GO:0018108	IDA
	positive regulation of DNA biosynthetic process	GO:2000573	IMP
	insulin receptor signaling pathway	GO:0008286	TAS
	positive regulation of proteolysis	GO:0045862	IMP
	alveolar secondary septum development	GO:0061144	IEA
	epidermal growth factor receptor signaling pathway	GO:0007173	TAS
	positive regulation of cell proliferation	GO:0008284	IGI
	glucose homeostasis	GO:0042593	ISS
	phosphate ion homeostasis	GO:0055062	ISS
	neurotrophin TRK receptor signaling pathway	GO:0048011	TAS
	Fc-epsilon receptor signaling pathway	GO:0038095	TAS
	cell migration	GO:0016477	IMP
	positive regulation of metalloenzyme activity	GO:0048554	IMP
	regulation of extracellular matrix disassembly	GO:0010715	IMP
	fibroblast growth factor receptor signaling pathway	GO:0008543	TAS
	organ induction	GO:0001759	IEA
	protein autophosphorylation	GO:0046777	IDA
	phosphatidylinositol-mediated signaling	GO:0048015	TAS
	regulation of cholesterol homeostasis	GO:2000188	ISS
	regulation of lipid metabolic process	GO:0019216	ISS
	positive regulation of ERK1 and ERK2 cascade	GO:0070374	IMP
	innate immune response	GO:0045087	TAS
	regulation of bile acid biosynthetic process	GO:0070857	IMP
	positive regulation of gene expression	GO:0010628	IMP
Subcellular Localization
	endosome	GO:0005768	IEA
	nucleoplasm	GO:0005654	IDA
	Golgi apparatus	GO:0005794	IDA
	cytoplasm	GO:0005737	IDA
	transport vesicle	GO:0030133	IDA
	plasma membrane	GO:0005886	TAS
	integral component of plasma membrane	GO:0005887	IDA
	endoplasmic reticulum	GO:0005783	IDA
	extracellular region	GO:0005576	IEA
	cell-cell junction	GO:0005911	IDA

Experiment description of studies that identified FGFR4 in exosomes

Experiment ID

1203

MISEV standards
✔ EM	EV Biophysical techniques
✔ SDCBP\|FLOT1\|CD9\|CD81\|CD63\|EPCAM\|GAPDH\|LAMP1\|TFRC\|CD151\|CD82\|LAMP2\|RAB35\|TSG101\|FLOT2\|RAB5B\|ICAM1\|RAB5A	EV Enriched markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

34887515

Organism

Homo sapiens

Experiment description

Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets

Authors

Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.

Journal name

Nat Cell Biol

Publication year

2021

Sample

Colorectal cancer cells

Sample name

DiFi

Isolation/purification methods

Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation

Flotation density

Molecules identified in the study

Protein
miRNA

Methods used in the study

Western blotting
Mass spectrometry
RNA sequencing

Experiment ID

224

MISEV standards
✔ EM\|AFM	EV Biophysical techniques
✔ Alix\|TSG101\|CD63\|CD81	EV Enriched markers
✔ GOLGA2	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

25944692

Organism

Homo sapiens

Experiment description

Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes

Authors

Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S

Journal name

Oncotarget

Publication year

2015

Sample

Neuroblastoma cells

Sample name

SH-SY5Y

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
OptiPrep density gradient

Flotation density

1.10 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry
Western blotting

Protein-protein interactions for FGFR4

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

FGF8

Invivo

Homo sapiens

FGF19

9965

Reconstituted Complex

Homo sapiens

FGF2

2247

Invitro

Homo sapiens

FGF1

Invivo		Homo sapiens
Invivo		Homo sapiens
Invitro		Homo sapiens
Invitro		Homo sapiens

View the network

Pathways in which FGFR4 is involved

Pathway	Evidence	Source
betaKlotho-mediated ligand binding	TAS	Reactome
Constitutive Signaling by Aberrant PI3K in Cancer	TAS	Reactome
FGFR4 ligand binding and activation	TAS	Reactome
FGFR4 mutant receptor activation	TAS	Reactome
FRS2-mediated FGFR4 signaling	TAS	Reactome
Negative regulation of FGFR4 signaling	TAS	Reactome
Phospholipase C-mediated cascade; FGFR4	TAS	Reactome
PI-3K cascade:FGFR4	TAS	Reactome
PI3K Cascade	TAS	Reactome
PI3K Cascade	TAS	Reactome
PIP3 activates AKT signaling	TAS	Reactome
SHC-mediated cascade:FGFR4	TAS	Reactome
Signaling by FGFR4 mutants	TAS	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here