Gene description for FGFR4
Gene name fibroblast growth factor receptor 4
Gene symbol FGFR4
Other names/aliases CD334
JTK2
TKF
Species Homo sapiens
 Database cross references - FGFR4
ExoCarta ExoCarta_2264
Vesiclepedia VP_2264
Entrez Gene 2264
HGNC 3691
MIM 134935
UniProt P22455  
 FGFR4 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Neuroblastoma cells 25944692    
 Gene ontology annotations for FGFR4
Molecular Function
    GPI-linked ephrin receptor activity GO:0005004 IEA
    transmembrane-ephrin receptor activity GO:0005005 IEA
    epidermal growth factor receptor activity GO:0005006 IEA
    fibroblast growth factor receptor activity GO:0005007 IBA
    fibroblast growth factor receptor activity GO:0005007 IDA
    fibroblast growth factor receptor activity GO:0005007 IGI
    hepatocyte growth factor receptor activity GO:0005008 IEA
    insulin receptor activity GO:0005009 IEA
    insulin-like growth factor receptor activity GO:0005010 IEA
    macrophage colony-stimulating factor receptor activity GO:0005011 IEA
    platelet-derived growth factor alpha-receptor activity GO:0005018 IEA
    platelet-derived growth factor beta-receptor activity GO:0005019 IEA
    stem cell factor receptor activity GO:0005020 IEA
    vascular endothelial growth factor receptor activity GO:0005021 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    heparin binding GO:0008201 IDA
    boss receptor activity GO:0008288 IEA
    fibroblast growth factor binding GO:0017134 IBA
    fibroblast growth factor binding GO:0017134 IDA
    fibroblast growth factor binding GO:0017134 IPI
    histone H3Y41 kinase activity GO:0035401 IEA
    placental growth factor receptor activity GO:0036332 IEA
    protein tyrosine kinase collagen receptor activity GO:0038062 IEA
    brain-derived neurotrophic factor receptor activity GO:0060175 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    epidermal growth factor receptor signaling pathway GO:0007173 IEA
    multicellular organism development GO:0007275 IBA
    positive regulation of cell population proliferation GO:0008284 IDA
    positive regulation of cell population proliferation GO:0008284 IGI
    insulin receptor signaling pathway GO:0008286 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 IDA
    fibroblast growth factor receptor signaling pathway GO:0008543 IGI
    fibroblast growth factor receptor signaling pathway GO:0008543 IPI
    positive regulation of gene expression GO:0010628 IMP
    regulation of extracellular matrix disassembly GO:0010715 IMP
    cell migration GO:0016477 IMP
    peptidyl-tyrosine phosphorylation GO:0018108 IDA
    regulation of lipid metabolic process GO:0019216 ISS
    brain-derived neurotrophic factor receptor signaling pathway GO:0031547 IEA
    platelet-derived growth factor receptor-alpha signaling pathway GO:0035790 IEA
    platelet-derived growth factor receptor-beta signaling pathway GO:0035791 IEA
    vascular endothelial growth factor receptor-1 signaling pathway GO:0036323 IEA
    collagen-activated tyrosine kinase receptor signaling pathway GO:0038063 IEA
    vascular endothelial growth factor signaling pathway GO:0038084 IEA
    Kit signaling pathway GO:0038109 IEA
    macrophage colony-stimulating factor signaling pathway GO:0038145 IEA
    glucose homeostasis GO:0042593 ISS
    cholesterol homeostasis GO:0042632 ISS
    positive regulation of catalytic activity GO:0043085 IMP
    positive regulation of proteolysis GO:0045862 IMP
    protein autophosphorylation GO:0046777 IDA
    insulin-like growth factor receptor signaling pathway GO:0048009 IEA
    hepatocyte growth factor receptor signaling pathway GO:0048012 IEA
    ephrin receptor signaling pathway GO:0048013 IEA
    phosphate ion homeostasis GO:0055062 ISS
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IBA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IMP
    regulation of bile acid biosynthetic process GO:0070857 IBA
    regulation of bile acid biosynthetic process GO:0070857 IMP
    response to bile acid GO:1903412 IMP
    positive regulation of DNA biosynthetic process GO:2000573 IMP
Subcellular Localization
    extracellular region GO:0005576 IEA
    endosome GO:0005768 IEA
    endoplasmic reticulum GO:0005783 IDA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 IDA
    transport vesicle GO:0030133 IDA
    receptor complex GO:0043235 IBA
 Experiment description of studies that identified FGFR4 in sEVs
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for FGFR4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LY86  
Affinity Capture-MS Homo sapiens
2 NTF3  
Affinity Capture-MS Homo sapiens
3 RYK 6259
Affinity Capture-MS Homo sapiens
4 CFC1  
Affinity Capture-MS Homo sapiens
5 CRLF2 64109
Affinity Capture-MS Homo sapiens
6 LRRIQ1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 GPIHBP1  
Affinity Capture-MS Homo sapiens
8 PRTN3 5657
Affinity Capture-MS Homo sapiens
9 FCGR3B  
Affinity Capture-MS Homo sapiens
10 CCL22 6367
Affinity Capture-MS Homo sapiens
11 LIPG 9388
Affinity Capture-MS Homo sapiens
12 FZD7 8324
Affinity Capture-MS Homo sapiens
13 ERBB2 2064
PCA Homo sapiens
14 LILRA5  
Affinity Capture-MS Homo sapiens
15 PPP3CA 5530
Two-hybrid Homo sapiens
16 TMEM59 9528
Affinity Capture-MS Homo sapiens
17 TGFBR2 7048
Affinity Capture-MS Homo sapiens
18 TMEM87A 25963
Affinity Capture-MS Homo sapiens
19 SCGB2A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 TCTN1  
Affinity Capture-MS Homo sapiens
21 MARCH1  
Affinity Capture-MS Homo sapiens
22 UQCC1 55245
Affinity Capture-MS Homo sapiens
23 NRSN1  
Affinity Capture-MS Homo sapiens
24 FCGRT 2217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 IGLL5 100423062
Affinity Capture-MS Homo sapiens
26 PTPN6 5777
Two-hybrid Homo sapiens
27 GGH 8836
Affinity Capture-MS Homo sapiens
28 PTPN11 5781
Two-hybrid Homo sapiens
29 PTH2R  
Affinity Capture-MS Homo sapiens
30 CBLN4  
Affinity Capture-MS Homo sapiens
31 TRGV3  
Affinity Capture-MS Homo sapiens
32 PDGFRA 5156
Affinity Capture-MS Homo sapiens
33 BTLA  
Affinity Capture-MS Homo sapiens
34 TMEM30B 161291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 PRG2  
Affinity Capture-MS Homo sapiens
36 CX3CL1  
Affinity Capture-MS Homo sapiens
37 PI15  
Affinity Capture-MS Homo sapiens
38 CST9L  
Affinity Capture-MS Homo sapiens
39 FGF19 9965
Reconstituted Complex Homo sapiens
40 CLEC4A  
Affinity Capture-MS Homo sapiens
41 FBXO2 26232
Affinity Capture-MS Homo sapiens
42 TMX1 81542
Affinity Capture-MS Homo sapiens
43 LYZL1  
Affinity Capture-MS Homo sapiens
44 FBXO6 26270
Affinity Capture-MS Homo sapiens
45 PIK3R1 5295
Negative Genetic Homo sapiens
46 NCR3  
Affinity Capture-MS Homo sapiens
47 WDR25  
Affinity Capture-MS Homo sapiens
48 KLK2  
Affinity Capture-MS Homo sapiens
49 INS 3630
Affinity Capture-MS Homo sapiens
50 ELSPBP1  
Affinity Capture-MS Homo sapiens
51 EVI5  
Affinity Capture-MS Homo sapiens
52 KCNE3  
Affinity Capture-MS Homo sapiens
53 TM2D3  
Affinity Capture-MS Homo sapiens
54 C1QB 713
Affinity Capture-MS Homo sapiens
55 IDS  
Affinity Capture-MS Homo sapiens
56 NXPH3  
Affinity Capture-MS Homo sapiens
57 FGF5  
Co-localization Homo sapiens
58 SDF2L1 23753
Affinity Capture-MS Homo sapiens
59 CRLF1 9244
Affinity Capture-MS Homo sapiens
60 EPHA7 2045
Affinity Capture-MS Homo sapiens
61 PTPN12 5782
Two-hybrid Homo sapiens
62 CDK9 1025
Affinity Capture-MS Homo sapiens
63 C1orf54  
Affinity Capture-MS Homo sapiens
64 SLC39A5 283375
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 DNAJB9 4189
Affinity Capture-MS Homo sapiens
66 GXYLT1 283464
Affinity Capture-MS Homo sapiens
67 LYPD1  
Affinity Capture-MS Homo sapiens
68 EDN3  
Affinity Capture-MS Homo sapiens
69 DNASE1L1 1774
Affinity Capture-MS Homo sapiens
70 TDGF1  
Affinity Capture-MS Homo sapiens
71 SRC 6714
Negative Genetic Homo sapiens
72 EPDR1 54749
Affinity Capture-MS Homo sapiens
73 UGT1A10  
Affinity Capture-MS Homo sapiens
74 RTN4R 65078
Affinity Capture-MS Homo sapiens
75 ADAMTS12 81792
Affinity Capture-MS Homo sapiens
76 DPEP2  
Affinity Capture-MS Homo sapiens
77 PTPRR  
Two-hybrid Homo sapiens
78 MRAP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 LYPD4  
Affinity Capture-MS Homo sapiens
80 IL5RA  
Affinity Capture-MS Homo sapiens
81 SIRPD  
Affinity Capture-MS Homo sapiens
82 FGF1  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
83 TNFRSF10A 8797
Affinity Capture-MS Homo sapiens
84 IL22RA1 58985
Negative Genetic Homo sapiens
85 EDAR  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which FGFR4 is involved
PathwayEvidenceSource
betaKlotho-mediated ligand binding TAS Reactome
Constitutive Signaling by Aberrant PI3K in Cancer TAS Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
Downstream signaling of activated FGFR4 TAS Reactome
FGFR4 ligand binding and activation TAS Reactome
FGFR4 mutant receptor activation TAS Reactome
FRS-mediated FGFR4 signaling TAS Reactome
IGF1R signaling cascade TAS Reactome
Insulin receptor signalling cascade TAS Reactome
Intracellular signaling by second messengers TAS Reactome
IRS-mediated signalling TAS Reactome
IRS-related events triggered by IGF1R TAS Reactome
MAPK family signaling cascades TAS Reactome
MAPK1/MAPK3 signaling TAS Reactome
Negative regulation of FGFR4 signaling TAS Reactome
Negative regulation of the PI3K/AKT network TAS Reactome
Phospholipase C-mediated cascade; FGFR4 TAS Reactome
PI-3K cascade:FGFR4 TAS Reactome
PI3K Cascade TAS Reactome
PI3K/AKT Signaling in Cancer TAS Reactome
PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling TAS Reactome
PIP3 activates AKT signaling TAS Reactome
RAF/MAP kinase cascade TAS Reactome
SHC-mediated cascade:FGFR4 TAS Reactome
Signal Transduction TAS Reactome
Signaling by FGFR TAS Reactome
Signaling by FGFR in disease TAS Reactome
Signaling by FGFR4 TAS Reactome
Signaling by FGFR4 in disease TAS Reactome
Signaling by Insulin receptor TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R) TAS Reactome





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