Gene description for ADAMTS12
Gene name ADAM metallopeptidase with thrombospondin type 1 motif, 12
Gene symbol ADAMTS12
Other names/aliases PRO4389
Species Homo sapiens
 Database cross references - ADAMTS12
ExoCarta ExoCarta_81792
Vesiclepedia VP_81792
Entrez Gene 81792
HGNC 14605
MIM 606184
UniProt P58397  
 ADAMTS12 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for ADAMTS12
Molecular Function
    metalloendopeptidase activity GO:0004222 IBA
    metalloendopeptidase activity GO:0004222 IDA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    chondrocyte differentiation GO:0002062 IEA
    proteolysis GO:0006508 IBA
    cell-matrix adhesion GO:0007160 IMP
    cell migration GO:0016477 IMP
    proteoglycan catabolic process GO:0030167 IDA
    extracellular matrix organization GO:0030198 IBA
    collagen fibril organization GO:0030199 IEA
    negative regulation of chondrocyte differentiation GO:0032331 IDA
    ossification involved in bone maturation GO:0043931 IEA
    regulation of inflammatory response GO:0050727 IDA
    proteolysis involved in protein catabolic process GO:0051603 IDA
    proteolysis involved in protein catabolic process GO:0051603 IMP
    cellular response to interleukin-1 GO:0071347 IMP
    cellular response to tumor necrosis factor GO:0071356 IMP
    cellular response to BMP stimulus GO:0071773 IDA
    regulation of endothelial tube morphogenesis GO:1901509 IDA
    negative regulation of hepatocyte growth factor receptor signaling pathway GO:1902203 IDA
    negative regulation of cellular response to vascular endothelial growth factor stimulus GO:1902548 IDA
    negative regulation of cellular response to hepatocyte growth factor stimulus GO:2001113 IDA
Subcellular Localization
    extracellular region GO:0005576 IEA
    extracellular matrix GO:0031012 IBA
 Experiment description of studies that identified ADAMTS12 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for ADAMTS12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UGGT2 55757
Affinity Capture-MS Homo sapiens
2 ADAMTS7  
Affinity Capture-MS Homo sapiens
3 IL17RA 23765
Affinity Capture-MS Homo sapiens
4 TP53BP2  
Two-hybrid Homo sapiens
5 PDS5B 23047
Cross-Linking-MS (XL-MS) Homo sapiens
6 P4HTM  
Affinity Capture-MS Homo sapiens
7 RUFY3  
Affinity Capture-MS Homo sapiens
8 COIL  
Two-hybrid Homo sapiens
9 MAN2B2 23324
Affinity Capture-MS Homo sapiens
10 MYL6 4637
Two-hybrid Homo sapiens
11 CCDC36  
Two-hybrid Homo sapiens
12 ARSA 410
Affinity Capture-MS Homo sapiens
13 SQSTM1 8878
Affinity Capture-MS Homo sapiens
14 CALM2 805
Two-hybrid Homo sapiens
15 PAM 5066
Affinity Capture-MS Homo sapiens
16 MISP 126353
Affinity Capture-MS Homo sapiens
17 HIST1H2BJ 8970
Cross-Linking-MS (XL-MS) Homo sapiens
18 CDK5R2 8941
Cross-Linking-MS (XL-MS) Homo sapiens
19 ANKRD40 91369
Affinity Capture-MS Homo sapiens
20 FKBP9 11328
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 LOC102724334 102724334
Cross-Linking-MS (XL-MS) Homo sapiens
22 HIST2H2BF 440689
Cross-Linking-MS (XL-MS) Homo sapiens
23 SNRK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 TUBB1 81027
Affinity Capture-MS Homo sapiens
25 HIST1H2BO 8348
Cross-Linking-MS (XL-MS) Homo sapiens
26 FGFR3 2261
Affinity Capture-MS Homo sapiens
27 TRIM14 9830
Two-hybrid Homo sapiens
28 GALNT18  
Affinity Capture-MS Homo sapiens
29 UBR1 197131
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 HIST1H2BN 8341
Cross-Linking-MS (XL-MS) Homo sapiens
31 ARSG 22901
Affinity Capture-MS Homo sapiens
32 LRP6 4040
Affinity Capture-MS Homo sapiens
33 SP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 DUOX2 50506
Cross-Linking-MS (XL-MS) Homo sapiens
35 CLSTN3 9746
Affinity Capture-MS Homo sapiens
36 OLFM2  
Affinity Capture-MS Homo sapiens
37 MEOX2  
Two-hybrid Homo sapiens
38 HYAL2 8692
Affinity Capture-MS Homo sapiens
39 P3H3 10536
Affinity Capture-MS Homo sapiens
40 FGFR4 2264
Affinity Capture-MS Homo sapiens
41 ART5  
Affinity Capture-MS Homo sapiens
42 USF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 HIST1H2BK 85236
Cross-Linking-MS (XL-MS) Homo sapiens
44 DNAJC30  
Affinity Capture-MS Homo sapiens
45 CYLC1 1538
Cross-Linking-MS (XL-MS) Homo sapiens
46 HIST1H2BD 3017
Cross-Linking-MS (XL-MS) Homo sapiens
47 USF2 7392
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 PPM1A 5494
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 HCLS1 3059
Cross-Linking-MS (XL-MS) Homo sapiens
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