Gene description for PPM1A
Gene name protein phosphatase, Mg2+/Mn2+ dependent, 1A
Gene symbol PPM1A
Other names/aliases PP2C-ALPHA
PP2CA
PP2Calpha
Species Homo sapiens
 Database cross references - PPM1A
ExoCarta ExoCarta_5494
Entrez Gene 5494
HGNC 9275
MIM 606108
UniProt P35813  
 PPM1A identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for PPM1A
Molecular Function
    manganese ion binding GO:0030145 IEA
    protein binding GO:0005515 IPI
    signal transducer activity GO:0004871 IMP
    R-SMAD binding GO:0070412 IPI
    protein C-terminus binding GO:0008022 IEA
    magnesium ion binding GO:0000287 IEA
    protein serine/threonine phosphatase activity GO:0004722 TAS
    calmodulin-dependent protein phosphatase activity GO:0033192 IDA
Biological Process
    protein dephosphorylation GO:0006470 IMP
    dephosphorylation GO:0016311 IDA
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 IDA
    positive regulation of Wnt signaling pathway GO:0030177 IDA
    negative regulation of SMAD protein complex assembly GO:0010991 IDA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 TAS
    insulin receptor signaling pathway GO:0008286 TAS
    negative regulation of I-kappaB kinase/NF-kappaB signaling GO:0043124 IMP
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 IMP
    transcription, DNA-templated GO:0006351 TAS
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    peptidyl-threonine dephosphorylation GO:0035970 IDA
    Wnt signaling pathway GO:0016055 IDA
    transforming growth factor beta receptor signaling pathway GO:0007179 TAS
    cell cycle arrest GO:0007050 TAS
    N-terminal protein myristoylation GO:0006499 ISS
    gene expression GO:0010467 TAS
    negative regulation of NF-kappaB import into nucleus GO:0042347 IMP
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
Subcellular Localization
    voltage-gated calcium channel complex GO:0005891 IEA
    membrane GO:0016020 ISS
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IDA
    neuron projection GO:0043005 IEA
 Experiment description of studies that identified PPM1A in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PPM1A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GRM3 2913
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
2 LXN  
Affinity Capture-MS Homo sapiens
3 DVL2 1856
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PPM1A is involved
PathwayEvidenceSource
Downregulation of SMAD2/3:SMAD4 transcriptional activity TAS Reactome
Regulation of AMPK activity via LKB1 TAS Reactome





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