Gene description for PAK1
Gene name p21 protein (Cdc42/Rac)-activated kinase 1
Gene symbol PAK1
Other names/aliases PAKalpha
Species Homo sapiens
 Database cross references - PAK1
ExoCarta ExoCarta_5058
Vesiclepedia VP_5058
Entrez Gene 5058
HGNC 8590
MIM 602590
UniProt Q13153  
 PAK1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PAK1
Molecular Function
    protein kinase binding GO:0019901 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    protein kinase activity GO:0004672 IDA
    protein serine/threonine kinase activity GO:0004674 TAS
    collagen binding GO:0005518 IPI
    Rac GTPase binding GO:0048365 IBA
Biological Process
    positive regulation of peptidyl-serine phosphorylation GO:0033138 IDA
    apoptotic process GO:0006915 IEA
    neuromuscular junction development GO:0007528 IEA
    dendrite development GO:0016358 IEA
    positive regulation of stress fiber assembly GO:0051496 IMP
    positive regulation of JUN kinase activity GO:0043507 IMP
    positive regulation of intracellular estrogen receptor signaling pathway GO:0033148 IDA
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    activation of protein kinase activity GO:0032147 IBA
    regulation of apoptotic process GO:0042981 IBA
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    negative regulation of cell proliferation involved in contact inhibition GO:0060244 IMP
    actin cytoskeleton reorganization GO:0031532 IMP
    protein autophosphorylation GO:0046777 IDA
    response to hypoxia GO:0001666 IEA
    innate immune response GO:0045087 TAS
    T cell costimulation GO:0031295 TAS
    exocytosis GO:0006887 IEA
    cellular response to insulin stimulus GO:0032869 IEA
    cell migration GO:0016477 IBA
    branching morphogenesis of an epithelial tube GO:0048754 IMP
    ephrin receptor signaling pathway GO:0048013 TAS
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    positive regulation of protein phosphorylation GO:0001934 IMP
    neuron projection morphogenesis GO:0048812 ISS
    protein phosphorylation GO:0006468 IDA
    Rho protein signal transduction GO:0007266 IBA
    receptor clustering GO:0043113 IEA
    MAPK cascade GO:0000165 IDA
    axon guidance GO:0007411 TAS
    wound healing GO:0042060 IMP
    T cell receptor signaling pathway GO:0050852 TAS
    regulation of mitotic cell cycle GO:0007346 IBA
    stress-activated protein kinase signaling cascade GO:0031098 IBA
Subcellular Localization
    filamentous actin GO:0031941 NAS
    protein complex GO:0043234 IDA
    Golgi apparatus GO:0005794 IDA
    ruffle membrane GO:0032587 IEA
    growth cone GO:0030426 IEA
    dendrite GO:0030425 ISS
    Z disc GO:0030018 ISS
    nuclear membrane GO:0031965 ISS
    cytoplasm GO:0005737 IDA
    intercalated disc GO:0014704 ISS
    cytosol GO:0005829 TAS
    cell-cell junction GO:0005911 IDA
    focal adhesion GO:0005925 IEA
    ruffle GO:0001726 IDA
    plasma membrane GO:0005886 TAS
    axon GO:0030424 ISS
 Experiment description of studies that identified PAK1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PAK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDC2L2  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
2 ARHGEF7 8874
Invivo Homo sapiens
Invitro Homo sapiens
3 LIMK1 3984
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 ABI3 51225
Reconstituted Complex Homo sapiens
5 ARHGEF2 9181
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
6 MYLK 4638
Invitro Homo sapiens
7 NCK1 4690
Invitro Homo sapiens
8 CDC42 998
Reconstituted Complex Homo sapiens
9 ARPC1B 10095
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 PAK1 5058
Invivo Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
11 NCK2 8440
Invivo Homo sapiens
Invitro Homo sapiens
12 COL1A1 1277
Invitro Homo sapiens
13 PKLR 5313
Affinity Capture-Western Homo sapiens
14 ERBB2 2064
Affinity Capture-Western Homo sapiens
15 CDK5R1  
Invivo Homo sapiens
16 RAF1 5894
Invivo Homo sapiens
Invitro Homo sapiens
17 HGS 9146
Invivo Homo sapiens
18 ARHGEF6 9459
Invivo Homo sapiens
19 RAC1 5879
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
20 AKT1 207
Reconstituted Complex Homo sapiens
21 BMX  
Invivo Homo sapiens
Invitro Homo sapiens
22 PLCG1 5335
Reconstituted Complex Homo sapiens
23 PAK1IP1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
24 DYNLL1 8655
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
25 RHOJ 57381
Invitro Homo sapiens
Two-hybrid Homo sapiens
26 PPM1F 9647
Invivo Homo sapiens
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