Gene description for RAF1
Gene name Raf-1 proto-oncogene, serine/threonine kinase
Gene symbol RAF1
Other names/aliases CMD1NN
CRAF
NS5
Raf-1
c-Raf
Species Homo sapiens
 Database cross references - RAF1
ExoCarta ExoCarta_5894
Vesiclepedia VP_5894
Entrez Gene 5894
HGNC 9829
MIM 164760
UniProt P04049  
 RAF1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for RAF1
Molecular Function
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IPI
    MAP kinase kinase kinase activity GO:0004709 IEA
    mitogen-activated protein kinase kinase binding GO:0031434 IEA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein heterodimerization activity GO:0046982 IEA
    identical protein binding GO:0042802 IPI
    protein kinase activity GO:0004672 TAS
    ATP binding GO:0005524 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    axon guidance GO:0007411 TAS
    protein phosphorylation GO:0006468 TAS
    apoptotic process GO:0006915 TAS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    cell proliferation GO:0008283 TAS
    innate immune response GO:0045087 TAS
    insulin receptor signaling pathway GO:0008286 TAS
    activation of adenylate cyclase activity GO:0007190 NAS
    MAPK cascade GO:0000165 TAS
    regulation of cell motility GO:2000145 TAS
    transmembrane transport GO:0055085 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    heart development GO:0007507 IEA
    response to hypoxia GO:0001666 IEA
    positive regulation of peptidyl-serine phosphorylation GO:0033138 IDA
    wound healing GO:0042060 TAS
    platelet activation GO:0030168 TAS
    Ras protein signal transduction GO:0007265 TAS
    negative regulation of cell proliferation GO:0008285 IDA
    ion transmembrane transport GO:0034220 TAS
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
    blood coagulation GO:0007596 TAS
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    regulation of Rho protein signal transduction GO:0035023 TAS
    signal transduction GO:0007165 TAS
    activation of MAPKK activity GO:0000186 TAS
    negative regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902042 IEA
    synaptic transmission GO:0007268 TAS
    somatic stem cell maintenance GO:0035019 IEA
    death-inducing signaling complex assembly GO:0071550 IEA
    negative regulation of apoptotic process GO:0043066 IDA
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    regulation of cell differentiation GO:0045595 TAS
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 TAS
    intermediate filament cytoskeleton organization GO:0045104 IEA
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    negative regulation of protein complex assembly GO:0031333 IDA
    regulation of apoptotic process GO:0042981 TAS
Subcellular Localization
    cytoplasm GO:0005737 ISS
    plasma membrane GO:0005886 TAS
    pseudopodium GO:0031143 IEA
    cytosol GO:0005829 TAS
    mitochondrial outer membrane GO:0005741 TAS
    Golgi apparatus GO:0005794 IPI
    nucleus GO:0005634 IEA
 Experiment description of studies that identified RAF1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 191
MISEV standards
✘ EV Biophysical techniques
✔
Alix|CD81|CD9
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RAF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
2 PHB 5245
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
3 SFN 2810
Affinity Capture-MS Homo sapiens
4 CDC25A  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
5 LCK 3932
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
6 PRKCE 5581
Affinity Capture-Western Homo sapiens
7 CNKSR1  
Reconstituted Complex Homo sapiens
8 MAP3K1 4214
Invivo Homo sapiens
Invitro Homo sapiens
9 CCT3 7203
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
10 CFLAR  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
11 SPRY2 10253
Affinity Capture-Western Homo sapiens
12 SHOC2 8036
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 YWHAZ 7534
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
14 PAK1 5058
Invivo Homo sapiens
Invitro Homo sapiens
15 MAPK1 5594
Affinity Capture-Western Homo sapiens
16 RB1 5925
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
17 GNG4  
Reconstituted Complex Homo sapiens
18 YWHAE 7531
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
19 RASD2  
Reconstituted Complex Homo sapiens
20 PRKG1 5592
Affinity Capture-Western Homo sapiens
21 PRKCZ 5590
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
22 MAPK3 5595
Affinity Capture-Western Homo sapiens
23 HRAS 3265
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
24 NR3C1 2908
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
25 YWHAB 7529
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
26 GRB10 2887
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
27 MAP2K2 5605
Affinity Capture-MS Homo sapiens
28 STK26 51765
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
29 PDGFRB 5159
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
30 PEBP1 5037
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
31 RBL2 5934
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
32 MAP2K1 5604
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
33 MRAS 22808
Reconstituted Complex Homo sapiens
34 JAK2 3717
Reconstituted Complex Homo sapiens
35 CNKSR2 22866
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
36 SRC 6714
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
37 Hras 15461
Reconstituted Complex Mus musculus
38 PHKA2 5256
Two-hybrid Homo sapiens
39 RRAS2 22800
Invivo Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
40 MAP3K5 4217
Invitro Homo sapiens
Invivo Homo sapiens
41 MAPK7  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
42 RHEB 6009
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
43 LRPAP1 4043
Two-hybrid Homo sapiens
44 NRAS 4893
Affinity Capture-Western Homo sapiens
45 BCL2  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
46 FYN 2534
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
47 BAG1 573
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
48 JAK1 3716
Invivo Homo sapiens
49 SPRY4 81848
Affinity Capture-Western Homo sapiens
50 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
51 YWHAQ 10971
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
52 RAP1A 5906
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
53 BCL2L1 598
Reconstituted Complex Homo sapiens
54 PEBP4  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
55 MAPK8IP3  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
56 KRAS 3845
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
57 ERAS  
Affinity Capture-Western Homo sapiens
58 AR 367
Co-purification Homo sapiens
59 BRAF  
Invivo Homo sapiens
60 YWHAG 7532
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
61 MYC  
Reconstituted Complex Homo sapiens
62 AKT1 207
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
63 TSC22D3  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
64 DMPK  
Invivo Homo sapiens
65 VAV1 7409
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
66 OIP5  
Two-hybrid Homo sapiens
67 VDAC1 7416
Affinity Capture-Western Homo sapiens
68 GNB2 2783
Affinity Capture-Western Homo sapiens
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