Gene description for RAF1
Gene name Raf-1 proto-oncogene, serine/threonine kinase
Gene symbol RAF1
Other names/aliases CMD1NN
CRAF
NS5
Raf-1
c-Raf
Species Homo sapiens
 Database cross references - RAF1
ExoCarta ExoCarta_5894
Vesiclepedia VP_5894
Entrez Gene 5894
HGNC 9829
MIM 164760
UniProt P04049  
 RAF1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for RAF1
Molecular Function
    protein kinase activity GO:0004672 EXP
    protein serine/threonine kinase activity GO:0004674 IDA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    MAP kinase kinase kinase activity GO:0004709 IBA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    enzyme binding GO:0019899 IPI
    small GTPase binding GO:0031267 IPI
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    identical protein binding GO:0042802 IPI
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    metal ion binding GO:0046872 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    MAPK cascade GO:0000165 IBA
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 TAS
    apoptotic process GO:0006915 TAS
    signal transduction GO:0007165 TAS
    activation of adenylate cyclase activity GO:0007190 IDA
    negative regulation of cell population proliferation GO:0008285 IDA
    insulin receptor signaling pathway GO:0008286 IEA
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 IEA
    Schwann cell development GO:0014044 IEA
    thyroid gland development GO:0030878 IEA
    negative regulation of protein-containing complex assembly GO:0031333 IDA
    positive regulation of peptidyl-serine phosphorylation GO:0033138 IDA
    somatic stem cell population maintenance GO:0035019 IEA
    regulation of Rho protein signal transduction GO:0035023 TAS
    insulin secretion involved in cellular response to glucose stimulus GO:0035773 IEA
    response to muscle stretch GO:0035994 IEA
    ERBB2-ERBB3 signaling pathway GO:0038133 IEA
    wound healing GO:0042060 TAS
    myelination GO:0042552 IEA
    regulation of apoptotic process GO:0042981 TAS
    negative regulation of apoptotic process GO:0043066 IDA
    positive regulation of MAPK cascade GO:0043410 IDA
    type B pancreatic cell proliferation GO:0044342 IEA
    intermediate filament cytoskeleton organization GO:0045104 IEA
    regulation of cell differentiation GO:0045595 TAS
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    insulin-like growth factor receptor signaling pathway GO:0048009 IEA
    neurotrophin TRK receptor signaling pathway GO:0048011 IEA
    thymus development GO:0048538 IEA
    face development GO:0060324 IEA
    type II interferon-mediated signaling pathway GO:0060333 TAS
    death-inducing signaling complex assembly GO:0071550 IEA
    negative regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902042 IEA
    negative regulation of cysteine-type endopeptidase activity GO:2000117 TAS
    regulation of cell motility GO:2000145 TAS
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISS
    mitochondrion GO:0005739 IBA
    mitochondrial outer membrane GO:0005741 TAS
    Golgi apparatus GO:0005794 IPI
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    pseudopodium GO:0031143 IEA
 Experiment description of studies that identified RAF1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RAF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RBM38  
Affinity Capture-MS Homo sapiens
2 TRAPPC2 6399
Affinity Capture-MS Homo sapiens
3 CNKSR1  
Reconstituted Complex Homo sapiens
4 XIAP  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
5 CCT3 7203
Two-hybrid Homo sapiens
6 TMEM70  
Two-hybrid Homo sapiens
7 KSR1  
Biochemical Activity Homo sapiens
8 SHOC2 8036
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 SPATA7 55812
Affinity Capture-MS Homo sapiens
10 PKM 5315
Two-hybrid Homo sapiens
11 GPRASP2  
Affinity Capture-MS Homo sapiens
12 GNG4  
Reconstituted Complex Homo sapiens
13 YWHAE 7531
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 TRIM66  
Affinity Capture-MS Homo sapiens
15 SS18L2  
Affinity Capture-MS Homo sapiens
16 VDAC1 7416
Affinity Capture-Western Homo sapiens
17 HRAS 3265
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
18 KIF20A 10112
Affinity Capture-MS Homo sapiens
19 CALM1 801
Affinity Capture-Western Homo sapiens
20 GRB10 2887
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
21 PEBP1 5037
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
22 ARL8B 55207
Affinity Capture-MS Homo sapiens
23 NUP160 23279
Affinity Capture-MS Homo sapiens
24 PPP1CC 5501
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 MAP3K5 4217
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
26 RHEB 6009
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
27 PHAX  
Affinity Capture-MS Homo sapiens
28 NPLOC4 55666
Two-hybrid Homo sapiens
29 RFXANK  
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
30 WDR83  
Affinity Capture-Western Homo sapiens
31 FAM174A 345757
Affinity Capture-MS Homo sapiens
32 COPS5 10987
Affinity Capture-MS Homo sapiens
33 SRC 6714
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
34 ACTR1A 10121
Proximity Label-MS Homo sapiens
35 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
36 SCNN1B 6338
Affinity Capture-Western Homo sapiens
37 AMER1  
Affinity Capture-MS Homo sapiens
38 DYNLL1 8655
Affinity Capture-MS Homo sapiens
39 NCAPG2 54892
Affinity Capture-MS Homo sapiens
40 BIRC3 330
Reconstituted Complex Homo sapiens
41 YWHAH 7533
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 PHB 5245
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
43 Arrb2  
Affinity Capture-Western Rattus norvegicus
44 SFN 2810
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
FRET Homo sapiens
45 OIP5  
Two-hybrid Homo sapiens
46 TIMM50 92609
Affinity Capture-MS Homo sapiens
47 NPAS1  
Affinity Capture-MS Homo sapiens
48 CFLAR  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
49 EEF1A1 1915
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
50 DVL2 1856
Affinity Capture-MS Homo sapiens
51 LAMTOR1 55004
Proximity Label-MS Homo sapiens
52 HSPA8 3312
Affinity Capture-Western Homo sapiens
53 TMEM63B 55362
Affinity Capture-MS Homo sapiens
54 YWHAB 7529
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 LTK 4058
Affinity Capture-Western Homo sapiens
56 MAPK1 5594
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
57 RB1 5925
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
58 XPO1 7514
Affinity Capture-MS Homo sapiens
59 MAPK8IP3  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
60 IRS4 8471
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
61 VAV1 7409
Affinity Capture-Western Homo sapiens
62 MAPK3 5595
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
63 EMD 2010
Affinity Capture-MS Homo sapiens
64 Hras 15461
Reconstituted Complex Mus musculus
65 PDGFRB 5159
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
66 MRAS 22808
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
67 FAM96B 51647
Affinity Capture-MS Homo sapiens
68 NUP107 57122
Affinity Capture-MS Homo sapiens
69 RAB4A 5867
Proximity Label-MS Homo sapiens
70 RRAS2 22800
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
71 EEF1E1 9521
Affinity Capture-MS Homo sapiens
72 FYN 2534
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
73 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
74 SPRY4 81848
Affinity Capture-Western Homo sapiens
75 FLOT1 10211
Proximity Label-MS Homo sapiens
76 CRKL 1399
Co-localization Homo sapiens
77 LAS1L 81887
Affinity Capture-MS Homo sapiens
78 RAP1A 5906
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
79 EYA4 2070
Affinity Capture-Western Homo sapiens
80 NRAS 4893
Synthetic Lethality Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
81 PEBP4  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
82 YWHAQ 10971
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 BCL2L1 598
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
84 SPDYE4  
Affinity Capture-MS Homo sapiens
85 ERAS  
Affinity Capture-Western Homo sapiens
86 BRAF  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
87 DYNLL2 140735
Affinity Capture-MS Homo sapiens
88 BRAP  
Affinity Capture-Western Homo sapiens
89 MAPK8 5599
Two-hybrid Homo sapiens
90 Aldh4a1  
Affinity Capture-MS Mus musculus
91 MAD2L1 4085
Affinity Capture-MS Homo sapiens
92 Ksr1  
Affinity Capture-Western Mus musculus
93 PRKCE 5581
Affinity Capture-Western Homo sapiens
94 RMDN3 55177
Co-localization Homo sapiens
95 VCP 7415
Affinity Capture-MS Homo sapiens
96 BIRC2  
Reconstituted Complex Homo sapiens
97 STK3 6788
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
98 HSPA5 3309
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
99 COPS7A 50813
Two-hybrid Homo sapiens
100 MSH2 4436
Affinity Capture-MS Homo sapiens
101 YWHAZ 7534
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 IQCB1  
Affinity Capture-MS Homo sapiens
103 PRKAR1A 5573
Two-hybrid Homo sapiens
104 PRKCZ 5590
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
105 CUL3 8452
Affinity Capture-MS Homo sapiens
106 NR3C1 2908
Affinity Capture-Western Homo sapiens
107 EGFR 1956
Two-hybrid Homo sapiens
PCA Homo sapiens
108 MAP3K1 4214
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
109 RPGRIP1L 23322
Affinity Capture-MS Homo sapiens
110 METTL21B  
Affinity Capture-MS Homo sapiens
111 RIPK4  
Affinity Capture-MS Homo sapiens
112 TBXA2R 6915
Affinity Capture-Western Homo sapiens
113 Cep152  
Affinity Capture-MS Mus musculus
114 CNKSR2 22866
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
115 NXF1 10482
Affinity Capture-RNA Homo sapiens
116 YY1 7528
Two-hybrid Homo sapiens
117 AURKB 9212
Affinity Capture-MS Homo sapiens
118 RANBP9 10048
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
119 UBE2D2 7322
Biochemical Activity Homo sapiens
120 MAPK6  
Affinity Capture-MS Homo sapiens
121 Rfxank  
Two-hybrid Mus musculus
122 SYNPO 11346
Affinity Capture-MS Homo sapiens
123 RASD2  
Reconstituted Complex Homo sapiens
124 MYC  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
125 BAG1 573
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
126 JAK1 3716
Affinity Capture-Western Homo sapiens
127 GNB2 2783
Affinity Capture-Western Homo sapiens
128 RBL2 5934
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
129 CDK20  
Affinity Capture-MS Homo sapiens
130 MYBPC1  
Two-hybrid Homo sapiens
131 ILK 3611
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
132 BCL6  
Biochemical Activity Homo sapiens
133 HAX1  
Proximity Label-MS Homo sapiens
134 NEDD4 4734
Reconstituted Complex Homo sapiens
135 HSPD1 3329
Affinity Capture-MS Homo sapiens
136 AKT1 207
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
137 TSC22D3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
138 ARAF 369
Affinity Capture-MS Homo sapiens
139 AKAP1 8165
Proximity Label-MS Homo sapiens
140 PPP2CB 5516
Two-hybrid Homo sapiens
141 NEDD4L 23327
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
142 HUWE1 10075
Affinity Capture-Western Homo sapiens
143 EIF3D 8664
Affinity Capture-MS Homo sapiens
144 RBBP8  
Two-hybrid Homo sapiens
145 STUB1 10273
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
146 HIPK4  
Affinity Capture-MS Homo sapiens
147 CDC25A  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
148 MAP2K2 5605
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
149 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
150 CCT8 10694
Affinity Capture-MS Homo sapiens
151 SGK1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
152 SPRY2 10253
Affinity Capture-Western Homo sapiens
153 NUP133 55746
Affinity Capture-MS Homo sapiens
154 BAD  
Co-localization Homo sapiens
Co-localization Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
155 OPALIN  
Affinity Capture-MS Homo sapiens
156 CALU 813
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
157 DCAF8 50717
Two-hybrid Homo sapiens
158 CDC37 11140
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
159 LCK 3932
Affinity Capture-Western Homo sapiens
160 NPHP4 261734
Affinity Capture-MS Homo sapiens
161 PAK1 5058
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
162 PCDH7 5099
Affinity Capture-MS Homo sapiens
163 ITCH 83737
Affinity Capture-Western Homo sapiens
164 FYCO1 79443
Two-hybrid Homo sapiens
165 STK26 51765
Two-hybrid Homo sapiens
166 HSP90AA1 3320
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
167 FKBP5 2289
Affinity Capture-MS Homo sapiens
168 MAP2K1 5604
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
169 LMAN1 3998
Proximity Label-MS Homo sapiens
170 LATS1  
Affinity Capture-Western Homo sapiens
171 PACSIN3 29763
Two-hybrid Homo sapiens
172 Prkab1  
Biochemical Activity Rattus norvegicus
173 PHKA2 5256
Two-hybrid Homo sapiens
174 RBM23  
Affinity Capture-MS Homo sapiens
175 MAPK7  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
176 LRPAP1 4043
Two-hybrid Homo sapiens
177 MOV10 4343
Affinity Capture-RNA Homo sapiens
178 BCL2  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
179 PSMA5 5686
Affinity Capture-MS Homo sapiens
180 ACOX1 51
Affinity Capture-MS Homo sapiens
181 RRAS 6237
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
182 SSSCA1 10534
Affinity Capture-MS Homo sapiens
183 PRPF6 24148
Two-hybrid Homo sapiens
184 BRD1 23774
Affinity Capture-MS Homo sapiens
185 KRAS 3845
Two-hybrid Homo sapiens
Synthetic Lethality Homo sapiens
Synthetic Lethality Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
PCA Homo sapiens
186 LOX 4015
Reconstituted Complex Homo sapiens
187 SH3KBP1 30011
Affinity Capture-Western Homo sapiens
188 AR 367
Co-purification Homo sapiens
189 KBTBD4  
Affinity Capture-MS Homo sapiens
190 BIRC7  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
191 DNAJA1 3301
Affinity Capture-MS Homo sapiens
192 PURA 5813
Two-hybrid Homo sapiens
193 JAK2 3717
Reconstituted Complex Homo sapiens
194 RAB2A 5862
Proximity Label-MS Homo sapiens
195 Smn1 20595
Affinity Capture-MS Mus musculus
196 CUL4A 8451
Affinity Capture-MS Homo sapiens
197 PRKG1 5592
Affinity Capture-Western Homo sapiens
198 C9orf72  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RAF1 is involved
PathwayEvidenceSource
Adaptive Immune System TAS Reactome
C-type lectin receptors (CLRs) TAS Reactome
CD209 (DC-SIGN) signaling TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
Gain-of-function MRAS complexes activate RAF signaling TAS Reactome
GP1b-IX-V activation signalling TAS Reactome
Hemostasis TAS Reactome
IFNG signaling activates MAPKs TAS Reactome
Immune System TAS Reactome
Innate Immune System TAS Reactome
Interferon gamma signaling TAS Reactome
Interferon Signaling TAS Reactome
Ion channel transport TAS Reactome
MAP2K and MAPK activation TAS Reactome
MAPK family signaling cascades TAS Reactome
MAPK1/MAPK3 signaling TAS Reactome
Negative feedback regulation of MAPK pathway TAS Reactome
Negative regulation of MAPK pathway TAS Reactome
Oncogenic MAPK signaling TAS Reactome
Paradoxical activation of RAF signaling by kinase inactive BRAF TAS Reactome
Platelet activation, signaling and aggregation TAS Reactome
RAF activation TAS Reactome
RAF/MAP kinase cascade TAS Reactome
Rap1 signalling TAS Reactome
SHOC2 M1731 mutant abolishes MRAS complex function TAS Reactome
Signal Transduction TAS Reactome
Signaling by BRAF and RAF1 fusions TAS Reactome
Signaling by high-kinase activity BRAF mutants TAS Reactome
Signaling by moderate kinase activity BRAF mutants TAS Reactome
Signaling by MRAS-complex mutants TAS Reactome
Signaling by RAF1 mutants TAS Reactome
Signaling by RAS mutants TAS Reactome
Signaling downstream of RAS mutants TAS Reactome
Stimuli-sensing channels TAS Reactome
Transport of small molecules TAS Reactome





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