Gene description for EYA4
Gene name EYA transcriptional coactivator and phosphatase 4
Gene symbol EYA4
Other names/aliases CMD1J
DFNA10
Species Homo sapiens
 Database cross references - EYA4
ExoCarta ExoCarta_2070
Vesiclepedia VP_2070
Entrez Gene 2070
HGNC 3522
MIM 603550
UniProt O95677  
 EYA4 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for EYA4
Molecular Function
    protein tyrosine phosphatase activity GO:0004725 IBA
    non-membrane spanning protein tyrosine phosphatase activity GO:0004726 IEA
    protein binding GO:0005515 IPI
    protein tyrosine phosphatase activity, metal-dependent GO:0030946 IEA
    metal ion binding GO:0046872 IEA
    histone H2AXY142 phosphatase activity GO:0140793 IEA
Biological Process
    DNA repair GO:0006281 IEA
    chromatin remodeling GO:0006338 IEA
    visual perception GO:0007601 TAS
    anatomical structure morphogenesis GO:0009653 TAS
    cell differentiation GO:0030154 IBA
    positive regulation of DNA repair GO:0045739 IBA
    inner ear development GO:0048839 IEA
    negative regulation of extrinsic apoptotic signaling pathway in absence of ligand GO:2001240 IBA
Subcellular Localization
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified EYA4 in sEVs
1
Experiment ID 234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
4
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for EYA4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAPK1IP1L 93487
Affinity Capture-MS Homo sapiens
2 SEC24B 10427
Affinity Capture-MS Homo sapiens
3 EOGT 285203
Affinity Capture-MS Homo sapiens
4 TLX1  
Proximity Label-MS Homo sapiens
5 MAP2K2 5605
Affinity Capture-Western Homo sapiens
6 LUZP1 7798
Affinity Capture-MS Homo sapiens
7 AAK1 22848
Affinity Capture-MS Homo sapiens
8 AATK  
Two-hybrid Homo sapiens
9 BAG3 9531
Affinity Capture-MS Homo sapiens
10 NR4A1  
Affinity Capture-MS Homo sapiens
11 HELZ 9931
Affinity Capture-MS Homo sapiens
12 PLEC 5339
Affinity Capture-MS Homo sapiens
13 DDI2 84301
Affinity Capture-MS Homo sapiens
14 URGCP 55665
Affinity Capture-MS Homo sapiens
15 COQ5  
Affinity Capture-MS Homo sapiens
16 PIP 5304
Affinity Capture-MS Homo sapiens
17 PKP2 5318
Affinity Capture-MS Homo sapiens
18 ANAPC2 29882
Proximity Label-MS Homo sapiens
19 MAPK1 5594
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
20 FOXJ2  
Affinity Capture-MS Homo sapiens
21 KRT8 3856
Proximity Label-MS Homo sapiens
22 GATA2  
Proximity Label-MS Homo sapiens
23 MAPK3 5595
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
24 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
25 RPA3 6119
Affinity Capture-MS Homo sapiens
26 SLC25A15  
Affinity Capture-MS Homo sapiens
27 SIX4  
Affinity Capture-MS Homo sapiens
28 DVL3 1857
Affinity Capture-MS Homo sapiens
29 SIX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 SUPT5H 6829
Affinity Capture-MS Homo sapiens
31 UBE2D2 7322
Affinity Capture-MS Homo sapiens
32 MAP2K1 5604
Affinity Capture-Western Homo sapiens
33 PPIP5K2 23262
Affinity Capture-MS Homo sapiens
34 SOX15  
Proximity Label-MS Homo sapiens
35 GLO1 2739
Affinity Capture-MS Homo sapiens
36 ERCC6  
Affinity Capture-MS Homo sapiens
37 SOAT1 6646
Affinity Capture-MS Homo sapiens
38 PFDN2 5202
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 EYA4 2070
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 RPA2 6118
Affinity Capture-MS Homo sapiens
41 TRIM31  
Affinity Capture-MS Homo sapiens
42 ERBB3 2065
Two-hybrid Homo sapiens
43 SIX5  
Affinity Capture-MS Homo sapiens
44 TRIM69 140691
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
45 SCO1  
Affinity Capture-MS Homo sapiens
46 RAF1 5894
Affinity Capture-Western Homo sapiens
47 ERC1 23085
Affinity Capture-MS Homo sapiens
48 HNF1B  
Proximity Label-MS Homo sapiens
49 KRAS 3845
Affinity Capture-Western Homo sapiens
50 CCDC88A 55704
Affinity Capture-MS Homo sapiens
51 SP7  
Proximity Label-MS Homo sapiens
52 QSER1  
Affinity Capture-MS Homo sapiens
53 DCTPP1 79077
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 SCO2  
Affinity Capture-MS Homo sapiens
55 SIX2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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