Gene description for PKP2
Gene name plakophilin 2
Gene symbol PKP2
Other names/aliases ARVD9
Species Homo sapiens
 Database cross references - PKP2
ExoCarta ExoCarta_5318
Entrez Gene 5318
HGNC 9024
MIM 602861
UniProt Q99959  
 PKP2 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for PKP2
Molecular Function
    protein binding GO:0005515 IPI
    protein complex scaffold GO:0032947 IMP
    ion channel binding GO:0044325 ISS
    intermediate filament binding GO:0019215 IDA
    molecular_function GO:0003674 ND
    cell adhesive protein binding involved in bundle of His cell-Purkinje myocyte communication GO:0086083 IC
    protein kinase C binding GO:0005080 IPI
    sodium channel regulator activity GO:0017080 ISS
    alpha-catenin binding GO:0045294 IPI
Biological Process
    regulation of heart rate by cardiac conduction GO:0086091 IMP
    cardiac muscle cell action potential GO:0086001 ISS
    cell-cell signaling involved in cardiac conduction GO:0086019 IMP
    regulation of bicellular tight junction assembly GO:2000810 ISS
    negative regulation of cell migration GO:0030336 ISS
    cell communication by electrical coupling involved in cardiac conduction GO:0086064 ISS
    establishment of protein localization to plasma membrane GO:0090002 IMP
    heart development GO:0007507 ISS
    single organismal cell-cell adhesion GO:0016337 NAS
    negative regulation of cell proliferation GO:0008285 ISS
    positive regulation of sodium ion transport GO:0010765 ISS
    cardiac muscle cell action potential involved in contraction GO:0086002 IMP
    bundle of His cell-Purkinje myocyte adhesion involved in cell communication GO:0086073 IMP
    intermediate filament bundle assembly GO:0045110 IMP
    desmosome assembly GO:0002159 IMP
    ventricular cardiac muscle cell action potential GO:0086005 IMP
    maintenance of organ identity GO:0048496 IMP
    regulation of ventricular cardiac muscle cell action potential GO:0098911 IMP
    lipid homeostasis GO:0055088 ISS
    gap junction assembly GO:0016264 ISS
    adherens junction maintenance GO:0034334 ISS
    ventricular cardiac muscle tissue morphogenesis GO:0055010 IMP
Subcellular Localization
    adherens junction GO:0005912 ISS
    nucleus GO:0005634 NAS
    intermediate filament GO:0005882 ISS
    integral component of membrane GO:0016021 TAS
    desmosome GO:0030057 NAS
    intercalated disc GO:0014704 ISS
    nucleoplasm GO:0005654 IDA
    cell junction GO:0030054 IDA
    cell-cell junction GO:0005911 ISS
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified PKP2 in exosomes
1
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PKP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SFN 2810
Affinity Capture-MS Homo sapiens
2 KRT18 3875
Invitro Homo sapiens
3 DSC2 1824
Two-hybrid Homo sapiens
4 DSG1 1828
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
5 DSC1 1823
Two-hybrid Homo sapiens
6 KRT5 3852
Invitro Homo sapiens
7 JUP 3728
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
8 POLR3A 11128
Invitro Homo sapiens
9 DSG2 1829
Affinity Capture-Western Homo sapiens
10 DSP 1832
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which PKP2 is involved
No pathways found





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