Gene description for POLR3A
Gene name polymerase (RNA) III (DNA directed) polypeptide A, 155kDa
Gene symbol POLR3A
Other names/aliases ADDH
HLD7
RPC1
RPC155
hRPC155
Species Homo sapiens
 Database cross references - POLR3A
ExoCarta ExoCarta_11128
Vesiclepedia VP_11128
Entrez Gene 11128
HGNC 30074
MIM 614258
UniProt O14802  
 POLR3A identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for POLR3A
Molecular Function
    zinc ion binding GO:0008270 IEA
    DNA binding GO:0003677 IEA
    ribonucleoside binding GO:0032549 IEA
    DNA-directed RNA polymerase activity GO:0003899 NAS
    chromatin binding GO:0003682 IEA
    RNA polymerase III activity GO:0001056 IBA
Biological Process
    gene expression GO:0010467 TAS
    regulation of mitochondrion degradation GO:1903146 IMP
    positive regulation of protein targeting to mitochondrion GO:1903955 IMP
    defense response to virus GO:0051607 IEA
    transcription from RNA polymerase III promoter GO:0006383 TAS
    innate immune response GO:0045087 TAS
    transcription elongation from RNA polymerase III promoter GO:0006385 TAS
    transcription, DNA-templated GO:0006351 NAS
    termination of RNA polymerase III transcription GO:0006386 TAS
    positive regulation of interferon-beta production GO:0032728 IMP
    positive regulation of type I interferon production GO:0032481 TAS
Subcellular Localization
    DNA-directed RNA polymerase III complex GO:0005666 IDA
    membrane GO:0016020 IDA
    cytosol GO:0005829 TAS
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified POLR3A in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for POLR3A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 POLR3E 55718
Invivo Homo sapiens
Invitro Homo sapiens
2 PKP2 5318
Invitro Homo sapiens
View the network image/svg+xml



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