Gene description for POLR2E
Gene name polymerase (RNA) II (DNA directed) polypeptide E, 25kDa
Gene symbol POLR2E
Other names/aliases RPABC1
RPB5
XAP4
hRPB25
hsRPB5
Species Homo sapiens
 Database cross references - POLR2E
ExoCarta ExoCarta_5434
Vesiclepedia VP_5434
Entrez Gene 5434
HGNC 9192
MIM 180664
UniProt P19388  
 POLR2E identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Thymus 23844026    
 Gene ontology annotations for POLR2E
Molecular Function
    RNA polymerase I activity GO:0001054 IBA
    DNA binding GO:0003677 IEA
    protein binding GO:0005515 IPI
    RNA polymerase III activity GO:0001056 IBA
    RNA polymerase II activity GO:0001055 IBA
    DNA-directed RNA polymerase activity GO:0003899 TAS
Biological Process
    gene expression GO:0010467 TAS
    transcription from RNA polymerase II promoter GO:0006366 TAS
    viral process GO:0016032 TAS
    transcription elongation from RNA polymerase III promoter GO:0006385 TAS
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    transcription-coupled nucleotide-excision repair GO:0006283 TAS
    transcription from RNA polymerase I promoter GO:0006360 TAS
    positive regulation of viral transcription GO:0050434 TAS
    mRNA splicing, via spliceosome GO:0000398 TAS
    transcription elongation from RNA polymerase II promoter GO:0006368 TAS
    somatic stem cell maintenance GO:0035019 TAS
    piRNA metabolic process GO:0034587 TAS
    termination of RNA polymerase III transcription GO:0006386 TAS
    termination of RNA polymerase I transcription GO:0006363 TAS
    7-methylguanosine mRNA capping GO:0006370 TAS
    regulation of gene expression, epigenetic GO:0040029 TAS
    transcription initiation from RNA polymerase I promoter GO:0006361 TAS
    transcription elongation from RNA polymerase I promoter GO:0006362 TAS
    negative regulation of gene expression, epigenetic GO:0045814 TAS
    RNA splicing GO:0008380 TAS
    transcription from RNA polymerase III promoter GO:0006383 TAS
    innate immune response GO:0045087 TAS
    positive regulation of type I interferon production GO:0032481 TAS
    nucleotide-excision repair GO:0006289 TAS
    DNA repair GO:0006281 TAS
Subcellular Localization
    nucleus GO:0005634 IDA
    cytosol GO:0005829 TAS
    DNA-directed RNA polymerase I complex GO:0005736 IBA
    DNA-directed RNA polymerase III complex GO:0005666 IBA
    nucleoplasm GO:0005654 TAS
    DNA-directed RNA polymerase II, core complex GO:0005665 IDA
 Experiment description of studies that identified POLR2E in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for POLR2E
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 POLR2B 5431
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
2 C19orf2  
Reconstituted Complex Homo sapiens
3 POLR2C 5432
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
4 POLR2F  
Reconstituted Complex Homo sapiens
5 POLR2E 5434
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
6 MED9  
Affinity Capture-MS Homo sapiens
7 POLR2G 5436
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
8 TRG20  
Affinity Capture-MS Homo sapiens
9 BRDG1  
Affinity Capture-MS Homo sapiens
10 TAF15 8148
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
11 IXL  
Affinity Capture-MS Homo sapiens
12 POLR2K  
Reconstituted Complex Homo sapiens
13 GTF2F2 2963
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
14 POLR2H 5437
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
15 XRCC5 7520
Affinity Capture-Western Homo sapiens
16 CRSP7  
Affinity Capture-MS Homo sapiens
17 MED19  
Affinity Capture-MS Homo sapiens
18 POLR2A 5430
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
19 POLR2L 5441
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which POLR2E is involved
PathwayEvidenceSource
Abortive elongation of HIV-1 transcript in the absence of Tat TAS Reactome
Cytosolic sensors of pathogen-associated DNA TAS Reactome
Dual incision reaction in TC-NER TAS Reactome
Formation of HIV elongation complex in the absence of HIV Tat IEA Reactome
Formation of HIV-1 elongation complex containing HIV-1 Tat IEA Reactome
Formation of RNA Pol II elongation complex TAS Reactome
Formation of the Early Elongation Complex TAS Reactome
Formation of the HIV-1 Early Elongation Complex IEA Reactome
Formation of transcription-coupled NER (TC-NER) repair complex TAS Reactome
HIV elongation arrest and recovery IEA Reactome
HIV Transcription Initiation IEA Reactome
MicroRNA (miRNA) biogenesis TAS Reactome
mRNA Capping TAS Reactome
mRNA Splicing - Major Pathway TAS Reactome
mRNA Splicing - Minor Pathway TAS Reactome
NoRC negatively regulates rRNA expression IEA Reactome
Pausing and recovery of HIV elongation TAS Reactome
Pausing and recovery of Tat-mediated HIV elongation TAS Reactome
PIWI-interacting RNA (piRNA) biogenesis TAS Reactome
POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation TAS Reactome
Processing of Capped Intron-Containing Pre-mRNA TAS Reactome
RNA Pol II CTD phosphorylation and interaction with CE TAS Reactome
RNA Pol II CTD phosphorylation and interaction with CE IEA Reactome
RNA Polymerase I Chain Elongation TAS Reactome
RNA Polymerase I Promoter Escape TAS Reactome
RNA Polymerase I Transcription Initiation TAS Reactome
RNA Polymerase I Transcription Termination TAS Reactome
RNA Polymerase II HIV Promoter Escape IEA Reactome
RNA Polymerase II Pre-transcription Events TAS Reactome
RNA Polymerase II Promoter Escape TAS Reactome
RNA Polymerase II Transcription Elongation TAS Reactome
RNA Polymerase II Transcription Initiation TAS Reactome
RNA Polymerase II Transcription Initiation And Promoter Clearance TAS Reactome
RNA Polymerase II Transcription Pre-Initiation And Promoter Opening TAS Reactome
RNA Polymerase III Abortive And Retractive Initiation TAS Reactome
RNA Polymerase III Chain Elongation TAS Reactome
RNA Polymerase III Transcription Initiation From Type 1 Promoter TAS Reactome
RNA Polymerase III Transcription Initiation From Type 2 Promoter TAS Reactome
RNA Polymerase III Transcription Initiation From Type 3 Promoter TAS Reactome
RNA Polymerase III Transcription Termination TAS Reactome
Tat-mediated elongation of the HIV-1 transcript TAS Reactome
Tat-mediated HIV elongation arrest and recovery IEA Reactome
Transcription of the HIV genome IEA Reactome
Transcription-coupled NER (TC-NER) TAS Reactome
Transcriptional regulation by small RNAs TAS Reactome
Transcriptional regulation of pluripotent stem cells TAS Reactome
Viral Messenger RNA Synthesis TAS Reactome





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