Gene description for MED20
Gene name mediator complex subunit 20
Gene symbol MED20
Other names/aliases PRO0213
TRFP
Species Homo sapiens
 Database cross references - MED20
ExoCarta ExoCarta_9477
Entrez Gene 9477
HGNC 16840
MIM 612915
UniProt Q9H944  
 MED20 identified in exosomes derived from the following tissue/cell type
Thymus 23844026    
 Gene ontology annotations for MED20
Molecular Function
    DNA-directed RNA polymerase activity GO:0003899 NAS
    protein binding GO:0005515 IPI
    RNA polymerase II transcription cofactor activity GO:0001104 IEA
Biological Process
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    transcription, DNA-templated GO:0006351 NAS
    regulation of transcription from RNA polymerase II promoter GO:0006357 IEA
    gene expression GO:0010467 TAS
    transcription from RNA polymerase II promoter GO:0006366 TAS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    mediator complex GO:0016592 NAS
 Experiment description of studies that identified MED20 in exosomes
1
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MED20
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MED18  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
2 MED9  
Affinity Capture-MS Homo sapiens
3 TRG20  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
4 PPARGC1A  
Affinity Capture-Western Homo sapiens
5 IXL  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
6 CRSP7  
Affinity Capture-MS Homo sapiens
7 MED19  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
8 MED28  
Affinity Capture-MS Homo sapiens
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