Gene description for MED20
Gene name mediator complex subunit 20
Gene symbol MED20
Other names/aliases PRO0213
TRFP
Species Homo sapiens
 Database cross references - MED20
ExoCarta ExoCarta_9477
Vesiclepedia VP_9477
Entrez Gene 9477
HGNC 16840
MIM 612915
UniProt Q9H944  
 MED20 identified in exosomes derived from the following tissue/cell type
Thymus 23844026    
 Gene ontology annotations for MED20
Molecular Function
    transcription coactivator activity GO:0003713 IBA
    DNA-directed 5'-3' RNA polymerase activity GO:0003899 NAS
    protein binding GO:0005515 IPI
Biological Process
    DNA-templated transcription GO:0006351 NAS
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    transcription by RNA polymerase II GO:0006366 TAS
    positive regulation of transcription elongation by RNA polymerase II GO:0032968 NAS
    skeletal muscle cell differentiation GO:0035914 IEA
    RNA polymerase II preinitiation complex assembly GO:0051123 NAS
    positive regulation of transcription initiation by RNA polymerase II GO:0060261 NAS
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    mediator complex GO:0016592 IBA
    mediator complex GO:0016592 NAS
    core mediator complex GO:0070847 IPI
 Experiment description of studies that identified MED20 in exosomes
1
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MED20
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ADRBK1 156
Co-fractionation Homo sapiens
2 POLR2G 5436
Affinity Capture-MS Homo sapiens
3 MED17  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 ATF7  
Affinity Capture-Western Homo sapiens
5 MED6  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 MED29  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 GDF9 2661
Affinity Capture-MS Homo sapiens
8 MED9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 MED13L 23389
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 SCAF4 57466
Affinity Capture-MS Homo sapiens
11 MED24 9862
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 PSORS1C2  
Two-hybrid Homo sapiens
13 CDK8 1024
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 LMO2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 MED12L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 MED13 9969
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 CASP3 836
Affinity Capture-MS Homo sapiens
18 ODF3  
Affinity Capture-MS Homo sapiens
19 POLR2I 5438
Affinity Capture-MS Homo sapiens
20 FBXW7  
Affinity Capture-MS Homo sapiens
21 MED27  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 MED22  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 BRD4 23476
Affinity Capture-MS Homo sapiens
24 ATG16L1 55054
Affinity Capture-MS Homo sapiens
25 POLR2C 5432
Affinity Capture-MS Homo sapiens
26 POLR2M  
Affinity Capture-MS Homo sapiens
27 CCNC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 MED4 29079
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
29 POLR2K  
Affinity Capture-MS Homo sapiens
30 MED1 5469
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 MED7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 CD300C  
Two-hybrid Homo sapiens
33 APOA2 336
Affinity Capture-MS Homo sapiens
34 MED26  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 MED25  
Affinity Capture-MS Homo sapiens
36 MED31  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 CEBPA  
Proximity Label-MS Homo sapiens
38 GTPBP4 23560
Co-fractionation Homo sapiens
39 MED15  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 POLR2B 5431
Affinity Capture-MS Homo sapiens
41 RECQL5  
Affinity Capture-MS Homo sapiens
42 PGLS 25796
Co-fractionation Homo sapiens
43 POLR2H 5437
Affinity Capture-MS Homo sapiens
44 EPAS1  
Affinity Capture-MS Homo sapiens
45 POLR2E 5434
Affinity Capture-MS Homo sapiens
46 ACAD11 84129
Affinity Capture-MS Homo sapiens
47 RPRD2  
Affinity Capture-MS Homo sapiens
48 CDK19 23097
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 NR3C1 2908
Affinity Capture-Western Homo sapiens
50 YY1 7528
Two-hybrid Homo sapiens
51 POLR2D  
Affinity Capture-MS Homo sapiens
52 POLR2A 5430
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 MED10  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 PCBP1 5093
Affinity Capture-MS Homo sapiens
55 TADA2A  
Affinity Capture-MS Homo sapiens
56 CYB5R2 51700
Two-hybrid Homo sapiens
57 GABPB1  
Two-hybrid Homo sapiens
58 MED23 9439
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 MED14  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 BIRC5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 ZC3H13 23091
Affinity Capture-MS Homo sapiens
62 MED11  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 UCK2 7371
Affinity Capture-MS Homo sapiens
64 MED19  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 ESR2  
Reconstituted Complex Homo sapiens
66 POLR2F  
Affinity Capture-MS Homo sapiens
67 FBXL19  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 POLR2J  
Affinity Capture-MS Homo sapiens
69 POLR1B 84172
Two-hybrid Homo sapiens
70 POLR2L 5441
Affinity Capture-MS Homo sapiens
71 PIH1D2  
Two-hybrid Homo sapiens
72 MED8  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 RPAP2  
Affinity Capture-MS Homo sapiens
74 Med22  
Affinity Capture-MS Mus musculus
75 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
76 TKT 7086
Affinity Capture-MS Homo sapiens
77 ESR1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
78 MED30  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 KLF5  
Affinity Capture-Western Homo sapiens
80 MED16 10025
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 UBB 7314
Affinity Capture-MS Homo sapiens
82 MED18  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 MED21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 TCL1B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 MED12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 TRIM69 140691
Two-hybrid Homo sapiens
87 CEBPB  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
88 AR 367
Proximity Label-MS Homo sapiens
89 PPARGC1A  
Affinity Capture-Western Homo sapiens
90 ERP27  
Two-hybrid Homo sapiens
91 CEBPD  
Affinity Capture-Western Homo sapiens
92 MED28  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MED20 is involved
PathwayEvidenceSource
Adipogenesis TAS Reactome
Adipogenesis IEA Reactome
Developmental Biology TAS Reactome
Developmental Biology IEA Reactome
Disease TAS Reactome
Epigenetic regulation by WDR5-containing histone modifying complexes TAS Reactome
Epigenetic regulation by WDR5-containing histone modifying complexes IEA Reactome
Epigenetic regulation of adipogenesis genes by MLL3 and MLL4 complexes TAS Reactome
Epigenetic regulation of adipogenesis genes by MLL3 and MLL4 complexes IEA Reactome
Epigenetic regulation of gene expression TAS Reactome
Epigenetic regulation of gene expression IEA Reactome
Epigenetic regulation of gene expression by MLL3 and MLL4 complexes TAS Reactome
Epigenetic regulation of gene expression by MLL3 and MLL4 complexes IEA Reactome
Gene expression (Transcription) TAS Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway TAS Reactome
Infectious disease TAS Reactome
Metabolism TAS Reactome
Metabolism of lipids TAS Reactome
MLL4 and MLL3 complexes regulate expression of PPARG target genes in adipogenesis and hepatic steatosis TAS Reactome
MLL4 and MLL3 complexes regulate expression of PPARG target genes in adipogenesis and hepatic steatosis IEA Reactome
PPARA activates gene expression TAS Reactome
Regulation of lipid metabolism by PPARalpha TAS Reactome
Respiratory Syncytial Virus Infection Pathway TAS Reactome
RNA Polymerase II Transcription TAS Reactome
RSV-host interactions TAS Reactome
Transcriptional regulation of white adipocyte differentiation TAS Reactome
Transcriptional regulation of white adipocyte differentiation IEA Reactome
Viral Infection Pathways TAS Reactome





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