Gene description for NR3C1
Gene name nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Gene symbol NR3C1
Other names/aliases GCCR
GCR
GCRST
GR
GRL
Species Homo sapiens
 Database cross references - NR3C1
ExoCarta ExoCarta_2908
Vesiclepedia VP_2908
Entrez Gene 2908
HGNC 7978
MIM 138040
UniProt P04150  
 NR3C1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for NR3C1
Molecular Function
    glucocorticoid-activated RNA polymerase II transcription factor binding transcription factor activity GO:0038051 IDA
    RNA polymerase II core promoter proximal region sequence-specific DNA binding GO:0000978 IDA
    steroid binding GO:0005496 IDA
    steroid hormone binding GO:1990239 IDA
    sequence-specific DNA binding transcription factor activity GO:0003700 IDA
    zinc ion binding GO:0008270 IEA
    protein binding GO:0005515 IPI
    glucocorticoid receptor activity GO:0004883 TAS
    protein dimerization activity GO:0046983 IEA
    RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription GO:0001077 IDA
Biological Process
    adrenal gland development GO:0030325 IEA
    regulation of gluconeogenesis GO:0006111 IEA
    maternal behavior GO:0042711 IEA
    signal transduction GO:0007165 TAS
    positive regulation of neuron apoptotic process GO:0043525 IEA
    regulation of glucocorticoid biosynthetic process GO:0031946 IEA
    regulation of transcription, DNA-templated GO:0006355 IDA
    glucocorticoid metabolic process GO:0008211 IEA
    glucocorticoid receptor signaling pathway GO:0042921 IEA
    mammary gland duct morphogenesis GO:0060603 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    glucocorticoid mediated signaling pathway GO:0043402 IEA
    transcription from RNA polymerase II promoter GO:0006366 TAS
    cellular response to steroid hormone stimulus GO:0071383 IDA
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    negative regulation of glucocorticoid mediated signaling pathway GO:1900170 IEA
    gene expression GO:0010467 TAS
    transcription, DNA-templated GO:0006351 TAS
    chromatin modification GO:0016568 IEA
Subcellular Localization
    cytosol GO:0005829 IEA
    nucleoplasm GO:0005654 TAS
    nucleus GO:0005634 IDA
    membrane GO:0016020 IEA
    cytoplasm GO:0005737 IDA
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified NR3C1 in exosomes
1
Experiment ID 489
ISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
ISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
ISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
ISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NR3C1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
2 SFN 2810
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
3 RELA 5970
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
4 CALR 811
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
5 NCOA6  
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
6 SELENBP1 8991
Affinity Capture-MS Homo sapiens
7 NR2F6  
Invitro Homo sapiens
8 SMARCA2 6595
Invivo Homo sapiens
9 HSGT1  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
10 SMARCE1 6605
Affinity Capture-Western Homo sapiens
11 SVIL 6840
Two-hybrid Homo sapiens
12 TP53 7157
Invivo Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
13 RNF14 9604
Invivo Homo sapiens
Invitro Homo sapiens
14 HSPA1A  
Reconstituted Complex Homo sapiens
15 NR3C1 2908
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
16 NCOA1  
Invitro Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
17 DAXX  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
18 RAD9A  
Reconstituted Complex Homo sapiens
19 MED1 5469
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
20 NCOA3  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
21 TBPIP  
Affinity Capture-Western Homo sapiens
22 SMARCC1 6599
Affinity Capture-Western Homo sapiens
23 PRPF6 24148
Invivo Homo sapiens
24 DDC 1644
Reconstituted Complex Homo sapiens
25 STAT5B 6777
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
26 SMARCD1 6602
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
27 JUN 3725
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
28 TSG101 7251
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
29 NCOA2 10499
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
30 NR3C2  
Invivo Homo sapiens
Invitro Homo sapiens
31 POU1F1  
Reconstituted Complex Homo sapiens
32 STAT5A 6776
Affinity Capture-MS Homo sapiens
33 ONECUT1  
Invitro Homo sapiens
34 FKBP4 2288
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
35 CREBBP  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
36 ZBTB16  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
37 TGFB1I1 7041
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
38 ETS1  
Affinity Capture-MS Homo sapiens
39 NCOR2  
Invitro Homo sapiens
40 ADA 100
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
41 POU2F1 5451
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
42 TBP  
Reconstituted Complex Homo sapiens
43 PBX1 5087
Invivo Homo sapiens
44 RANBP9 10048
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
45 HOXB1  
Invivo Homo sapiens
46 TXN 7295
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
47 UBE2I 7329
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
48 HSPD1 3329
Two-hybrid Homo sapiens
49 SMARCA4 6597
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
50 NCOR1  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
51 TRIM28 10155
Invivo Homo sapiens
Invitro Homo sapiens
52 NCL 4691
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
53 SLC25A4 291
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
54 BAG1 573
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
55 RAN 5901
Two-hybrid Homo sapiens
56 NFKB1 4790
Reconstituted Complex Homo sapiens
57 HSP90AA1 3320
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
58 CEBPB  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
59 SMAD3 4088
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
60 TADA2L  
Reconstituted Complex Homo sapiens
61 DAP3 7818
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
62 PTGES3 10728
Invivo Homo sapiens
63 CRSP2  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
64 NRIP1 8204
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
65 RAF1 5894
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
66 DDX54 79039
Invitro Homo sapiens
67 PTMS 5763
Affinity Capture-MS Homo sapiens
68 HNRNPU 3192
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
69 CREB1  
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
70 POU2F2 5452
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
71 NFKB2 4791
Reconstituted Complex Homo sapiens
72 ARPC5 10092
Two-hybrid Homo sapiens
73 TDG 6996
Reconstituted Complex Homo sapiens
74 SUMO1 7341
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
75 PELP1 27043
Invitro Homo sapiens
76 STAT3 6774
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
77 PPARGC1A  
Invitro Homo sapiens
78 ETS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
79 TIF1  
Invivo Homo sapiens
80 CEBPA  
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
81 JARID1A  
Reconstituted Complex Homo sapiens
82 NEDD4L 23327
Reconstituted Complex Homo sapiens
83 IL1RN 3557
Affinity Capture-MS Homo sapiens
84 Psmc3ip  
Reconstituted Complex Rattus norvegicus
Two-hybrid Rattus norvegicus
85 AR 367
Invitro Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which NR3C1 is involved
PathwayEvidenceSource
BMAL1:CLOCK,NPAS2 activates circadian gene expression TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here