Gene description for SMCR8
Gene name Smith-Magenis syndrome chromosome region, candidate 8
Gene symbol SMCR8
Other names/aliases -
Species Homo sapiens
 Database cross references - SMCR8
ExoCarta ExoCarta_140775
Vesiclepedia VP_140775
Entrez Gene 140775
HGNC 17921
UniProt Q8TEV9  
 SMCR8 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for SMCR8
Molecular Function
    protein kinase inhibitor activity GO:0004860 IMP
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    GTPase activator activity GO:0005096 IMP
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IPI
Biological Process
    autophagy GO:0006914 IEA
    regulation of autophagy GO:0010506 IMP
    regulation of autophagy GO:0010506 NAS
    negative regulation of gene expression GO:0010629 IMP
    negative regulation of macroautophagy GO:0016242 IMP
    positive regulation of TOR signaling GO:0032008 IMP
    negative regulation of exocytosis GO:0045920 NAS
    negative regulation of immune response GO:0050777 NAS
    positive regulation of autophagosome maturation GO:1901098 IMP
    negative regulation of autophagosome assembly GO:1902902 IMP
    regulation of TORC1 signaling GO:1903432 IMP
Subcellular Localization
    chromatin GO:0000785 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 NAS
    guanyl-nucleotide exchange factor complex GO:0032045 IBA
    guanyl-nucleotide exchange factor complex GO:0032045 IDA
    guanyl-nucleotide exchange factor complex GO:0032045 IPI
    cell projection GO:0042995 IEA
    presynapse GO:0098793 ISS
    postsynapse GO:0098794 ISS
    Atg1/ULK1 kinase complex GO:1990316 IDA
 Experiment description of studies that identified SMCR8 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for SMCR8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 METTL10  
Affinity Capture-MS Homo sapiens
2 REEP5 7905
Proximity Label-MS Homo sapiens
3 RAB9A 9367
Proximity Label-MS Homo sapiens
4 SEC63 11231
Proximity Label-MS Homo sapiens
5 MAVS 57506
Proximity Label-MS Homo sapiens
6 RAB35 11021
Proximity Label-MS Homo sapiens
7 PHB2 11331
Proximity Label-MS Homo sapiens
8 GORASP1 64689
Proximity Label-MS Homo sapiens
9 DNAJC25 548645
Proximity Label-MS Homo sapiens
10 MTCH1 23787
Proximity Label-MS Homo sapiens
11 EBAG9 9166
Proximity Label-MS Homo sapiens
12 FTL 2512
Affinity Capture-MS Homo sapiens
13 RPN2 6185
Proximity Label-MS Homo sapiens
14 AIFM1 9131
Proximity Label-MS Homo sapiens
15 METTL7A 25840
Proximity Label-MS Homo sapiens
16 LAMTOR1 55004
Proximity Label-MS Homo sapiens
17 MARCKS 4082
Proximity Label-MS Homo sapiens
18 LAMP3  
Proximity Label-MS Homo sapiens
19 LRRC59 55379
Proximity Label-MS Homo sapiens
20 IMPDH1 3614
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 NUP155 9631
Proximity Label-MS Homo sapiens
22 KIAA1715 80856
Proximity Label-MS Homo sapiens
23 PXMP2  
Proximity Label-MS Homo sapiens
24 RAB5A 5868
Proximity Label-MS Homo sapiens
25 CRYAB 1410
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 HSD17B11 51170
Proximity Label-MS Homo sapiens
27 CKAP4 10970
Proximity Label-MS Homo sapiens
28 GOLGA1  
Proximity Label-MS Homo sapiens
29 MTCH2 23788
Proximity Label-MS Homo sapiens
30 BCAP31 10134
Proximity Label-MS Homo sapiens
31 HSD3B7 80270
Proximity Label-MS Homo sapiens
32 NR3C1 2908
Proximity Label-MS Homo sapiens
33 STIM1 6786
Proximity Label-MS Homo sapiens
34 GJD3  
Proximity Label-MS Homo sapiens
35 EMD 2010
Proximity Label-MS Homo sapiens
36 LAMP2 3920
Proximity Label-MS Homo sapiens
37 FKBP8 23770
Proximity Label-MS Homo sapiens
38 RB1CC1 9821
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 STX6 10228
Proximity Label-MS Homo sapiens
40 PTPN1 5770
Proximity Label-MS Homo sapiens
41 CXADR 1525
Proximity Label-MS Homo sapiens
42 GJA1 2697
Proximity Label-MS Homo sapiens
43 DUSP16  
Affinity Capture-MS Homo sapiens
44 LMAN1 3998
Proximity Label-MS Homo sapiens
45 OCIAD1 54940
Proximity Label-MS Homo sapiens
46 RAB4A 5867
Proximity Label-MS Homo sapiens
47 ATG13 9776
Two-hybrid Homo sapiens
48 RAB3B 5865
Proximity Label-MS Homo sapiens
49 KRTCAP3 200634
Affinity Capture-MS Homo sapiens
50 PRKACA 5566
Proximity Label-MS Homo sapiens
51 CAV1 857
Proximity Label-MS Homo sapiens
52 FIS1 51024
Proximity Label-MS Homo sapiens
53 CDC16 8881
Affinity Capture-MS Homo sapiens
54 FAM90A1  
Affinity Capture-MS Homo sapiens
55 SEC62 7095
Proximity Label-MS Homo sapiens
56 PIPSL 266971
Affinity Capture-MS Homo sapiens
57 B3GAT1  
Proximity Label-MS Homo sapiens
58 MAGEA9  
Affinity Capture-MS Homo sapiens
59 NTRK1 4914
Affinity Capture-MS Homo sapiens
60 CAMK2A 815
Affinity Capture-MS Homo sapiens
61 RAB11A 8766
Proximity Label-MS Homo sapiens
62 SSSCA1 10534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 PEX14 5195
Proximity Label-MS Homo sapiens
64 RAB7A 7879
Proximity Label-MS Homo sapiens
65 IMPDH2 3615
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 ULK1  
Two-hybrid Homo sapiens
67 DNAJC1 64215
Proximity Label-MS Homo sapiens
68 MFN2 9927
Proximity Label-MS Homo sapiens
69 RAB5C 5878
Proximity Label-MS Homo sapiens
70 C9orf72  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
71 FANCA  
Affinity Capture-Luminescence Homo sapiens
72 SSR1 6745
Proximity Label-MS Homo sapiens
73 MARCH5  
Proximity Label-MS Homo sapiens
74 DERL1 79139
Proximity Label-MS Homo sapiens
75 STX4 6810
Proximity Label-MS Homo sapiens
76 RHOT2 89941
Proximity Label-MS Homo sapiens
77 ELOVL5 60481
Proximity Label-MS Homo sapiens
78 AKAP1 8165
Proximity Label-MS Homo sapiens
79 SEC61B 10952
Proximity Label-MS Homo sapiens
80 EXD2  
Proximity Label-MS Homo sapiens
81 DNAJC16  
Proximity Label-MS Homo sapiens
82 ACBD5 91452
Proximity Label-MS Homo sapiens
83 RMDN3 55177
Proximity Label-MS Homo sapiens
84 SLC25A46 91137
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SMCR8 is involved
No pathways found





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