Gene description for MFN2
Gene name mitofusin 2
Gene symbol MFN2
Other names/aliases CMT2A
CMT2A2
CPRP1
HSG
MARF
Species Homo sapiens
 Database cross references - MFN2
ExoCarta ExoCarta_9927
Vesiclepedia VP_9927
Entrez Gene 9927
HGNC 16877
MIM 608507
UniProt O95140  
 MFN2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for MFN2
Molecular Function
    GTPase activity GO:0003924 IBA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    ubiquitin protein ligase binding GO:0031625 IPI
    membrane scission GTPase motor activity GO:1990606 IEA
Biological Process
    blastocyst formation GO:0001825 IEA
    protein targeting to mitochondrion GO:0006626 IDA
    apoptotic process GO:0006915 IEA
    response to unfolded protein GO:0006986 IEA
    mitochondrial membrane organization GO:0007006 IDA
    mitochondrial fusion GO:0008053 IBA
    mitochondrial fusion GO:0008053 IGI
    mitochondrial fusion GO:0008053 IMP
    mitochondrial fusion GO:0008053 ISS
    mitochondrial fusion GO:0008053 NAS
    aerobic respiration GO:0009060 IMP
    protein localization to phagophore assembly site GO:0034497 IDA
    negative regulation of Ras protein signal transduction GO:0046580 IDA
    camera-type eye morphogenesis GO:0048593 IEA
    negative regulation of smooth muscle cell proliferation GO:0048662 ISS
    mitochondrion localization GO:0051646 IBA
    mitochondrion localization GO:0051646 IDA
    type 2 mitophagy GO:0061734 ISS
    positive regulation of cold-induced thermogenesis GO:0120162 ISS
    positive regulation of vascular associated smooth muscle cell proliferation GO:1904707 IMP
    positive regulation of vascular associated smooth muscle cell apoptotic process GO:1905461 IMP
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial outer membrane GO:0005741 IBA
    mitochondrial outer membrane GO:0005741 ISS
    mitochondrial outer membrane GO:0005741 TAS
    cytosol GO:0005829 ISS
    microtubule cytoskeleton GO:0015630 IEA
 Experiment description of studies that identified MFN2 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MFN2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 PSMA6 5687
Proximity Label-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 TPGS1  
Proximity Label-MS Homo sapiens
5 MTCH1 23787
Proximity Label-MS Homo sapiens
6 PSMD6 9861
Proximity Label-MS Homo sapiens
7 TTC4  
Proximity Label-MS Homo sapiens
8 PSMD11 5717
Proximity Label-MS Homo sapiens
9 MFN1 55669
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 NUDCD3 23386
Proximity Label-MS Homo sapiens
11 PSMC3 5702
Proximity Label-MS Homo sapiens
12 TPM4 7171
Proximity Label-MS Homo sapiens
13 WDR41  
Proximity Label-MS Homo sapiens
14 SMCR8 140775
Proximity Label-MS Homo sapiens
15 PSMB6 5694
Proximity Label-MS Homo sapiens
16 PSMC6 5706
Proximity Label-MS Homo sapiens
17 MARCH5  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
18 VPS13D 55187
Proximity Label-MS Homo sapiens
19 PSMC2 5701
Proximity Label-MS Homo sapiens
20 STIP1 10963
Proximity Label-MS Homo sapiens
21 KRAS 3845
Negative Genetic Homo sapiens
22 EXD2  
Proximity Label-MS Homo sapiens
23 SLC25A46 91137
Proximity Label-MS Homo sapiens
24 MAVS 57506
Proximity Label-MS Homo sapiens
25 PSMB3 5691
Proximity Label-MS Homo sapiens
26 KIF14 9928
Affinity Capture-MS Homo sapiens
27 ADSL 158
Proximity Label-MS Homo sapiens
28 SLC25A38  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
29 VSIG1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 PSMB4 5692
Proximity Label-MS Homo sapiens
31 RBX1 9978
Cross-Linking-MS (XL-MS) Homo sapiens
32 PSMD4 5710
Proximity Label-MS Homo sapiens
33 PARK2  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
34 NBR1 4077
Proximity Label-MS Homo sapiens
35 PSMC1 5700
Proximity Label-MS Homo sapiens
36 THY1 7070
Affinity Capture-MS Homo sapiens
37 SNAP47 116841
Proximity Label-MS Homo sapiens
38 AKAP1 8165
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
39 SREBF2 6721
Negative Genetic Homo sapiens
40 NUDC 10726
Proximity Label-MS Homo sapiens
41 VCP 7415
Biochemical Activity Homo sapiens
Proximity Label-MS Homo sapiens
42 NTRK1 4914
Affinity Capture-MS Homo sapiens
43 TCHP  
Affinity Capture-Western Homo sapiens
44 AIP 9049
Proximity Label-MS Homo sapiens
45 MYH10 4628
Proximity Label-MS Homo sapiens
46 VCPIP1 80124
Proximity Label-MS Homo sapiens
47 PSMD2 5708
Proximity Label-MS Homo sapiens
48 RHOT2 89941
Proximity Label-MS Homo sapiens
49 NIPSNAP1 8508
Proximity Label-MS Homo sapiens
50 ST13 6767
Proximity Label-MS Homo sapiens
51 CACYBP 27101
Proximity Label-MS Homo sapiens
52 KDF1  
Affinity Capture-MS Homo sapiens
53 REEP5 7905
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 UBE2N 7334
Affinity Capture-Western Homo sapiens
55 PSMA3 5684
Proximity Label-MS Homo sapiens
56 GOLGA3 2802
Proximity Label-MS Homo sapiens
57 IPO5 3843
Proximity Label-MS Homo sapiens
58 TMEM160 54958
Proximity Label-MS Homo sapiens
59 GLMN 11146
Proximity Label-MS Homo sapiens
60 BAG2 9532
Proximity Label-MS Homo sapiens
61 OCRL 4952
Proximity Label-MS Homo sapiens
62 PRKAR2A 5576
Proximity Label-MS Homo sapiens
63 C11orf49  
Proximity Label-MS Homo sapiens
64 NAA15 80155
Proximity Label-MS Homo sapiens
65 ACTBL2 345651
Proximity Label-MS Homo sapiens
66 DIP2A 23181
Proximity Label-MS Homo sapiens
67 PSMA2 5683
Proximity Label-MS Homo sapiens
68 FKBP8 23770
Proximity Label-MS Homo sapiens
69 MYO19  
Proximity Label-MS Homo sapiens
70 NSFL1C 55968
Proximity Label-MS Homo sapiens
71 KIAA1524 57650
Proximity Label-MS Homo sapiens
72 GHITM 27069
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 PSMD7 5713
Proximity Label-MS Homo sapiens
74 OCIAD1 54940
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
75 NXF1 10482
Affinity Capture-RNA Homo sapiens
76 PRKCI 5584
Proximity Label-MS Homo sapiens
77 KIF1BP 26128
Proximity Label-MS Homo sapiens
78 TXNL1 9352
Proximity Label-MS Homo sapiens
79 PSMC5 5705
Proximity Label-MS Homo sapiens
80 PSMB5 5693
Proximity Label-MS Homo sapiens
81 MFN2 9927
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
82 CRYBG3  
Proximity Label-MS Homo sapiens
83 CLTC 1213
Proximity Label-MS Homo sapiens
84 PSMB1 5689
Proximity Label-MS Homo sapiens
85 LARP4 113251
Proximity Label-MS Homo sapiens
86 HUWE1 10075
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
87 TPM3 7170
Proximity Label-MS Homo sapiens
88 GBAS 2631
Proximity Label-MS Homo sapiens
89 CYP2C19  
Affinity Capture-MS Homo sapiens
90 GYPA  
Affinity Capture-MS Homo sapiens
91 TRAK2 66008
Affinity Capture-MS Homo sapiens
92 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
93 ALDH3A2 224
Proximity Label-MS Homo sapiens
94 PSMD1 5707
Proximity Label-MS Homo sapiens
95 TTC1 7265
Proximity Label-MS Homo sapiens
96 TDRKH  
Proximity Label-MS Homo sapiens
97 UBXN4 23190
Proximity Label-MS Homo sapiens
98 HDAC6 10013
Affinity Capture-Western Homo sapiens
99 PSMB7 5695
Proximity Label-MS Homo sapiens
100 CDC37 11140
Proximity Label-MS Homo sapiens
101 ZZEF1 23140
Proximity Label-MS Homo sapiens
102 EDEM1  
Affinity Capture-MS Homo sapiens
103 CFAP36 112942
Proximity Label-MS Homo sapiens
104 SQSTM1 8878
Proximity Label-MS Homo sapiens
105 FKBP4 2288
Proximity Label-MS Homo sapiens
106 PRKAR2B 5577
Proximity Label-MS Homo sapiens
107 PSMA1 5682
Proximity Label-MS Homo sapiens
108 UBB 7314
Proximity Label-MS Homo sapiens
109 PPP4R1L  
Affinity Capture-MS Homo sapiens
110 DNM1L 10059
Proximity Label-MS Homo sapiens
111 PSMA5 5686
Proximity Label-MS Homo sapiens
112 UBR4 23352
Proximity Label-MS Homo sapiens
113 PSMA7 5688
Proximity Label-MS Homo sapiens
114 MAPK9 5601
Affinity Capture-Western Homo sapiens
115 IBTK 25998
Proximity Label-MS Homo sapiens
116 PINK1  
Co-localization Homo sapiens
117 YKT6 10652
Proximity Label-MS Homo sapiens
118 PIH1D1  
Proximity Label-MS Homo sapiens
119 Park2  
Biochemical Activity Rattus norvegicus
120 C3orf18  
Affinity Capture-MS Homo sapiens
121 C9orf72  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
122 LRRC49  
Proximity Label-MS Homo sapiens
123 ATXN3 4287
Proximity Label-MS Homo sapiens
124 PSMC4 5704
Proximity Label-MS Homo sapiens
125 PSMD13 5719
Proximity Label-MS Homo sapiens
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