Gene description for NXF1
Gene name nuclear RNA export factor 1
Gene symbol NXF1
Other names/aliases MEX67
TAP
Species Homo sapiens
 Database cross references - NXF1
ExoCarta ExoCarta_10482
Entrez Gene 10482
HGNC 8071
MIM 602647
UniProt Q9UBU9  
 NXF1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Thymus 23844026    
 Gene ontology annotations for NXF1
Molecular Function
    single-stranded RNA binding GO:0003727 IDA
    mRNA binding GO:0003729 IEA
    nucleotide binding GO:0000166 IEA
    nucleocytoplasmic transporter activity GO:0005487 IEA
    protein binding GO:0005515 IPI
    poly(A) RNA binding GO:0044822 IDA
Biological Process
    mRNA export from nucleus GO:0006406 TAS
    poly(A)+ mRNA export from nucleus GO:0016973 IEA
    viral process GO:0016032 IEA
    gene expression GO:0010467 TAS
Subcellular Localization
    nuclear pore GO:0005643 IEA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IDA
    nuclear speck GO:0016607 IDA
    transcription export complex GO:0000346 IDA
    nuclear inclusion body GO:0042405 IEA
 Experiment description of studies that identified NXF1 in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NXF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SRRM1  
Invivo Homo sapiens
2 NXF1 10482
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 ELAVL4  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 NXT1  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
5 DHX9 1660
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
6 NUP214  
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
7 TNPO2 30000
Invivo Homo sapiens
8 RBM8A 9939
Reconstituted Complex Homo sapiens
9 MAGOH  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
10 NXT2  
Affinity Capture-MS Homo sapiens
11 U2AF1 7307
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
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