Gene description for U2AF1
Gene name U2 small nuclear RNA auxiliary factor 1
Gene symbol U2AF1
Other names/aliases FP793
RN
RNU2AF1
U2AF35
U2AFBP
Species Homo sapiens
 Database cross references - U2AF1
ExoCarta ExoCarta_7307
Entrez Gene 7307
HGNC 12453
MIM 191317
UniProt Q01081  
 U2AF1 identified in exosomes derived from the following tissue/cell type
Thymus 23844026    
 Gene ontology annotations for U2AF1
Molecular Function
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    poly(A) RNA binding GO:0044822 IDA
    nucleotide binding GO:0000166 IEA
Biological Process
    positive regulation of protein targeting to mitochondrion GO:1903955 IMP
    RNA splicing GO:0008380 TAS
    mRNA splicing, via spliceosome GO:0000398 TAS
    termination of RNA polymerase II transcription GO:0006369 TAS
    mRNA export from nucleus GO:0006406 TAS
    mRNA 3'-end processing GO:0031124 TAS
    regulation of mitochondrion degradation GO:1903146 IMP
    mRNA processing GO:0006397 TAS
    gene expression GO:0010467 TAS
    transcription from RNA polymerase II promoter GO:0006366 TAS
Subcellular Localization
    Cajal body GO:0015030 TAS
    spliceosomal complex GO:0005681 IDA
    nuclear speck GO:0016607 ISS
    nucleoplasm GO:0005654 TAS
    catalytic step 2 spliceosome GO:0071013 IDA
 Experiment description of studies that identified U2AF1 in exosomes
1
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for U2AF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NXF1 10482
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
2 RP9  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
3 SRSF1 6426
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
4 RNU12  
Affinity Capture-MS Homo sapiens
5 U2AF1L2  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
6 VCL 7414
Affinity Capture-MS Homo sapiens
7 RNU11 26824
Affinity Capture-MS Homo sapiens
8 SRSF2 6427
Invivo Homo sapiens
Two-hybrid Homo sapiens
9 ZNF265  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
10 U2AF2 11338
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
11 EPB41 2035
Affinity Capture-MS Homo sapiens
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