Gene description for SAP18
Gene name Sin3A-associated protein, 18kDa
Gene symbol SAP18
Other names/aliases 2HOR0202
SAP18P
Species Homo sapiens
 Database cross references - SAP18
ExoCarta ExoCarta_10284
Entrez Gene 10284
HGNC 10530
MIM 602949
UniProt O00422  
 SAP18 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for SAP18
Molecular Function
    protein binding GO:0005515 IPI
    poly(A) RNA binding GO:0044822 IDA
    transcription corepressor activity GO:0003714 TAS
Biological Process
    regulation of gene expression, epigenetic GO:0040029 TAS
    regulation of transcription from RNA polymerase II promoter GO:0006357 TAS
    mRNA processing GO:0006397 IEA
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 IMP
    negative regulation of mRNA splicing, via spliceosome GO:0048025 IDA
    gene expression GO:0010467 TAS
    RNA splicing GO:0008380 IEA
    transcription, DNA-templated GO:0006351 IEA
    chromatin organization GO:0006325 TAS
    negative regulation of gene expression, epigenetic GO:0045814 TAS
    negative regulation of nucleic acid-templated transcription GO:1903507 TAS
    positive regulation of apoptotic process GO:0043065 IDA
Subcellular Localization
    exon-exon junction complex GO:0035145 IDA
    nucleoplasm GO:0005654 TAS
    nuclear speck GO:0016607 IDA
    ASAP complex GO:0061574 IDA
    histone deacetylase complex GO:0000118 TAS
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified SAP18 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SAP18
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DDX3X 1654
Affinity Capture-MS Homo sapiens
2 SUFU  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
3 ATP5L 10632
Affinity Capture-MS Homo sapiens
4 POLE2  
Two-hybrid Homo sapiens
5 NSF 4905
Affinity Capture-MS Homo sapiens
6 YARS 8565
Affinity Capture-MS Homo sapiens
7 RPS3A 6189
Two-hybrid Homo sapiens
8 RGS10 6001
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 ARMT1 79624
Affinity Capture-MS Homo sapiens
10 Sufu  
Affinity Capture-Western Mus musculus
11 KIF1BP 26128
Affinity Capture-MS Homo sapiens
12 ZNFN1A1  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
13 DDOST 1650
Affinity Capture-MS Homo sapiens
14 RPN2 6185
Affinity Capture-MS Homo sapiens
15 HDAC1 3065
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
16 SIN3A  
Reconstituted Complex Homo sapiens
17 NOL12 79159
Two-hybrid Homo sapiens
18 TADA3L  
Two-hybrid Homo sapiens
19 EIF3E 3646
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SAP18 is involved
PathwayEvidenceSource
HDACs deacetylate histones TAS Reactome
NoRC negatively regulates rRNA expression IEA Reactome





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