Gene description for LOC255308
Gene name eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa pseudogene
Gene symbol LOC255308
Other names/aliases -
Species Homo sapiens
 Database cross references - LOC255308
ExoCarta ExoCarta_255308
Vesiclepedia VP_255308
Entrez Gene 255308
 LOC255308 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for LOC255308
Molecular Function
    tRNA binding GO:0000049 IBA
    translation initiation factor activity GO:0003743 IBA
    GTP binding GO:0005525 IEA
    membrane scission GTPase motor activity GO:1990606 IEA
Biological Process
    formation of translation preinitiation complex GO:0001731 IBA
Subcellular Localization
    eukaryotic translation initiation factor 2 complex GO:0005850 IBA
 Experiment description of studies that identified LOC255308 in exosomes
1
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for LOC255308
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PSMD14 10213
Affinity Capture-MS Homo sapiens
2 EIF2S1 1965
Co-fractionation Homo sapiens
3 SRSF1 6426
Co-fractionation Homo sapiens
4 NOP2 4839
Co-fractionation Homo sapiens
5 SAP18 10284
Co-fractionation Homo sapiens
6 C9orf72  
Affinity Capture-MS Homo sapiens
7 RPS24 6229
Co-fractionation Homo sapiens
8 RPS11 6205
Co-fractionation Homo sapiens
9 ZNF326 284695
Co-fractionation Homo sapiens
10 CDC123 8872
Co-fractionation Homo sapiens
11 RPS5 6193
Co-fractionation Homo sapiens
12 SOX2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which LOC255308 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here