Gene description for PHKA2
Gene name phosphorylase kinase, alpha 2 (liver)
Gene symbol PHKA2
Other names/aliases GSD9A
PHK
PYK
PYKL
XLG
XLG2
Species Homo sapiens
 Database cross references - PHKA2
ExoCarta ExoCarta_5256
Vesiclepedia VP_5256
Entrez Gene 5256
HGNC 8926
MIM 300798
UniProt P46019  
 PHKA2 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for PHKA2
Molecular Function
    phosphorylase kinase activity GO:0004689 TAS
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IEA
Biological Process
    carbohydrate metabolic process GO:0005975 TAS
    glycogen metabolic process GO:0005977 IEA
    generation of precursor metabolites and energy GO:0006091 TAS
    protein modification process GO:0036211 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    phosphorylase kinase complex GO:0005964 IBA
 Experiment description of studies that identified PHKA2 in exosomes
1
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PHKA2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MESDC2 23184
Affinity Capture-MS Homo sapiens
2 CEP57  
Affinity Capture-MS Homo sapiens
3 METTL18  
Affinity Capture-MS Homo sapiens
4 UBE2S  
Affinity Capture-MS Homo sapiens
5 ARRB2 409
Affinity Capture-MS Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 CDC14B 8555
Affinity Capture-MS Homo sapiens
8 TUBGCP2 10844
Affinity Capture-MS Homo sapiens
9 TOMM34 10953
Affinity Capture-MS Homo sapiens
10 ROCK1 6093
Affinity Capture-MS Homo sapiens
11 Ndc80  
Affinity Capture-MS Mus musculus
12 Mtx2  
Affinity Capture-MS Mus musculus
13 TP53 7157
Affinity Capture-MS Homo sapiens
14 KIAA0196 9897
Co-fractionation Homo sapiens
15 CCDC43 124808
Affinity Capture-MS Homo sapiens
16 ARL5A 26225
Affinity Capture-MS Homo sapiens
17 CALM2 805
Affinity Capture-MS Homo sapiens
18 FGF12  
Affinity Capture-MS Homo sapiens
19 LAMP1 3916
Affinity Capture-MS Homo sapiens
20 SAP18 10284
Affinity Capture-MS Homo sapiens
21 GYG2  
Affinity Capture-MS Homo sapiens
22 GOLT1B 51026
Affinity Capture-MS Homo sapiens
23 Rmdn3  
Affinity Capture-MS Mus musculus
24 DOK4 55715
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 Oxnad1  
Affinity Capture-MS Mus musculus
26 UBE3A 7337
Affinity Capture-MS Homo sapiens
27 EYA2  
Affinity Capture-MS Homo sapiens
28 PKN2 5586
Affinity Capture-MS Homo sapiens
29 CALM1 801
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 PHKG2 5261
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 BTF3L4 91408
Affinity Capture-MS Homo sapiens
32 SBDS 51119
Affinity Capture-MS Homo sapiens
33 PHKA1 5255
Affinity Capture-MS Homo sapiens
34 PUS7 54517
Affinity Capture-MS Homo sapiens
35 UBE3C 9690
Co-fractionation Homo sapiens
36 BAG6 7917
Affinity Capture-MS Homo sapiens
37 MPPED1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 ITGAV 3685
Affinity Capture-Western Homo sapiens
39 VRK1 7443
Affinity Capture-MS Homo sapiens
40 PPP1R12A 4659
Affinity Capture-MS Homo sapiens
41 MMS19 64210
Co-fractionation Homo sapiens
42 ROCK2 9475
Affinity Capture-MS Homo sapiens
43 Ppp1r3b  
Affinity Capture-MS Mus musculus
44 SMAD9  
Two-hybrid Homo sapiens
45 PHKB 5257
Affinity Capture-MS Homo sapiens
46 ARFGAP2 84364
Affinity Capture-MS Homo sapiens
47 STK25 10494
Affinity Capture-MS Homo sapiens
48 HUWE1 10075
Affinity Capture-MS Homo sapiens
49 RAF1 5894
Two-hybrid Homo sapiens
50 CMSS1  
Affinity Capture-MS Homo sapiens
51 CALM3 808
Affinity Capture-MS Homo sapiens
52 QRSL1  
Affinity Capture-MS Homo sapiens
53 PHKG1  
Affinity Capture-MS Homo sapiens
54 WBP4  
Affinity Capture-MS Homo sapiens
55 FOXK1 221937
Affinity Capture-MS Homo sapiens
56 TCEANC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 TULP3 7289
Affinity Capture-MS Homo sapiens
58 CHMP1A 5119
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PHKA2 is involved
PathwayEvidenceSource
Glycogen breakdown (glycogenolysis) TAS Reactome
Glycogen metabolism TAS Reactome
Metabolism TAS Reactome
Metabolism of carbohydrates TAS Reactome





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