Gene description for ITGAV
Gene name integrin, alpha V
Gene symbol ITGAV
Other names/aliases CD51
MSK8
VNRA
VTNR
Species Homo sapiens
 Database cross references - ITGAV
ExoCarta ExoCarta_3685
Vesiclepedia VP_3685
Entrez Gene 3685
HGNC 6150
MIM 193210
UniProt P06756  
 ITGAV identified in sEVs derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Bladder cancer cells 20224111    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 17956143    
Colorectal cancer cells 19837982    
Colorectal cancer cells 34887515    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Melanoma cells 22635005    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Plasma 36631249    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Prostate cancer cells 25857301    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for ITGAV
Molecular Function
    virus receptor activity GO:0001618 IEA
    opsonin binding GO:0001846 ISS
    fibronectin binding GO:0001968 IDA
    protease binding GO:0002020 IDA
    protein kinase C binding GO:0005080 ISS
    integrin binding GO:0005178 IBA
    integrin binding GO:0005178 IPI
    voltage-gated calcium channel activity GO:0005245 IDA
    protein binding GO:0005515 IPI
    coreceptor activity GO:0015026 TAS
    fibroblast growth factor binding GO:0017134 IDA
    C-X3-C chemokine binding GO:0019960 IDA
    insulin-like growth factor I binding GO:0031994 IDA
    neuregulin binding GO:0038132 IDA
    metal ion binding GO:0046872 IEA
    transforming growth factor beta binding GO:0050431 ISS
    extracellular matrix binding GO:0050840 IDA
    extracellular matrix protein binding GO:1990430 IDA
Biological Process
    angiogenesis GO:0001525 IBA
    angiogenesis GO:0001525 IEP
    vasculogenesis GO:0001570 IEA
    cell adhesion GO:0007155 IDA
    cell-matrix adhesion GO:0007160 IBA
    cell-matrix adhesion GO:0007160 IMP
    cell-matrix adhesion GO:0007160 NAS
    positive regulation of cytosolic calcium ion concentration GO:0007204 IEA
    integrin-mediated signaling pathway GO:0007229 IBA
    integrin-mediated signaling pathway GO:0007229 IDA
    integrin-mediated signaling pathway GO:0007229 IMP
    integrin-mediated signaling pathway GO:0007229 NAS
    positive regulation of cell population proliferation GO:0008284 IDA
    negative regulation of macrophage derived foam cell differentiation GO:0010745 IMP
    negative regulation of lipid storage GO:0010888 IMP
    cell migration GO:0016477 IMP
    positive regulation of cell migration GO:0030335 IEA
    cell-substrate adhesion GO:0031589 IMP
    negative regulation of lipid transport GO:0032369 IMP
    cell adhesion mediated by integrin GO:0033627 IBA
    cell adhesion mediated by integrin GO:0033627 IDA
    positive regulation of osteoblast proliferation GO:0033690 IEA
    heterotypic cell-cell adhesion GO:0034113 IMP
    substrate adhesion-dependent cell spreading GO:0034446 IDA
    wound healing, spreading of epidermal cells GO:0035313 NAS
    endodermal cell differentiation GO:0035987 IMP
    apolipoprotein A-I-mediated signaling pathway GO:0038027 IMP
    apoptotic cell clearance GO:0043277 IGI
    apoptotic cell clearance GO:0043277 ISS
    positive regulation of cell adhesion GO:0045785 IDA
    symbiont entry into host cell GO:0046718 IMP
    symbiont entry into host cell GO:0046718 TAS
    negative regulation of lipoprotein metabolic process GO:0050748 IMP
    regulation of phagocytosis GO:0050764 IDA
    negative chemotaxis GO:0050919 IMP
    positive regulation of small GTPase mediated signal transduction GO:0051057 IMP
    ERK1 and ERK2 cascade GO:0070371 ISS
    calcium ion transmembrane transport GO:0070588 IDA
    transforming growth factor beta production GO:0071604 IEA
    entry into host cell by a symbiont-containing vacuole GO:0085017 NAS
    extrinsic apoptotic signaling pathway in absence of ligand GO:0097192 ISS
    cell-cell adhesion GO:0098609 IBA
    positive regulation of intracellular signal transduction GO:1902533 IMP
    negative regulation of low-density lipoprotein receptor activity GO:1905598 IMP
    negative regulation of entry of bacterium into host cell GO:2000536 IDA
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 IMP
Subcellular Localization
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 NAS
    plasma membrane GO:0005886 TAS
    focal adhesion GO:0005925 HDA
    focal adhesion GO:0005925 IDA
    integrin complex GO:0008305 IBA
    integrin complex GO:0008305 IDA
    integrin complex GO:0008305 NAS
    external side of plasma membrane GO:0009897 IBA
    cell surface GO:0009986 HDA
    cell surface GO:0009986 IDA
    cell surface GO:0009986 ISS
    membrane GO:0016020 ISS
    lamellipodium membrane GO:0031258 IDA
    filopodium membrane GO:0031527 IDA
    microvillus membrane GO:0031528 IDA
    ruffle membrane GO:0032587 IDA
    integrin alphav-beta1 complex GO:0034682 IPI
    integrin alphav-beta3 complex GO:0034683 IDA
    integrin alphav-beta3 complex GO:0034683 IPI
    integrin alphav-beta5 complex GO:0034684 IDA
    integrin alphav-beta5 complex GO:0034684 IPI
    integrin alphav-beta6 complex GO:0034685 IDA
    integrin alphav-beta6 complex GO:0034685 IPI
    integrin alphav-beta8 complex GO:0034686 IDA
    integrin alphav-beta8 complex GO:0034686 IPI
    specific granule membrane GO:0035579 TAS
    alphav-beta3 integrin-PKCalpha complex GO:0035866 NAS
    alphav-beta3 integrin-IGF-1-IGF1R complex GO:0035867 IDA
    alphav-beta3 integrin-HMGB1 complex GO:0035868 IDA
    phagocytic vesicle GO:0045335 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified ITGAV in sEVs
1
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 76
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
5
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 1181
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 1182
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 494
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
14
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
15
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
16
Experiment ID 498
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
17
Experiment ID 20
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
18
Experiment ID 21
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
19
Experiment ID 1203
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
20
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
21
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
22
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
23
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
24
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
25
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
26
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
27
Experiment ID 1371
MISEV standards
Biophysical techniques
✔ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name PLC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
28
Experiment ID 1372
MISEV standards
Biophysical techniques
✔ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name PLC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
29
Experiment ID 1373
MISEV standards
Biophysical techniques
✔ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name 97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
30
Experiment ID 1374
MISEV standards
Biophysical techniques
✔ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name 97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
31
Experiment ID 1377
MISEV standards
Biophysical techniques
✔ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name 97H
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
32
Experiment ID 1378
MISEV standards
Biophysical techniques
✔ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name 97H
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
33
Experiment ID 1381
MISEV standards
Biophysical techniques
✔ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name 97H
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
34
Experiment ID 1382
MISEV standards
Biophysical techniques
✔ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name 97H
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
35
Experiment ID 1383
MISEV standards
Biophysical techniques
✔ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name 97H
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
36
Experiment ID 1384
MISEV standards
Biophysical techniques
✔ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name 97H
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
37
Experiment ID 1385
MISEV standards
Biophysical techniques
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name PLC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
38
Experiment ID 1386
MISEV standards
Biophysical techniques
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name PLC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
39
Experiment ID 1387
MISEV standards
Biophysical techniques
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name PLC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
40
Experiment ID 1388
MISEV standards
Biophysical techniques
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name PLC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
41
Experiment ID 200
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22635005    
Organism Homo sapiens
Experiment description Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET.
Authors "Peinado H, Aleckovic M, Lavotshkin S, Matei I, Costa-Silva B, Moreno-Bueno G, Hergueta-Redondo M, Williams C, Garcia-Santos G, Ghajar C, Nitadori-Hoshino A, Hoffman C, Badal K, Garcia BA, Callahan MK, Yuan J, Martins VR, Skog J, Kaplan RN, Brady MS, Wolchok JD, Chapman PB, Kang Y, Bromberg J, Lyden D."
Journal name Nat Med
Publication year 2012
Sample Melanoma cells
Sample name B16-F10
SK-MEL-202
SK-MEL035
SK-MEL-265
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
42
Experiment ID 257
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
43
Experiment ID 258
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
44
Experiment ID 259
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
45
Experiment ID 260
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
46
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
47
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
48
Experiment ID 224
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
49
Experiment ID 211
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
50
Experiment ID 212
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
51
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
52
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
53
Experiment ID 1370
MISEV standards
Biophysical techniques
✔ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Immuno-EM
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Plasma
Sample name Hepatocellular carcinoma patients
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Immuno-EM
54
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
55
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
56
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
57
Experiment ID 250
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857301    
Organism Homo sapiens
Experiment description Large oncosomes contain distinct protein cargo and represent a separate functional class of tumor-derived extracellular vesicles.
Authors "Minciacchi VR, You S, Spinelli C, Morley S, Zandian M, Aspuria PJ, Cavallini L, Ciardiello C, Reis Sobreiro M, Morello M, Kharmate G, Jang SC, Kim DK, Hosseini-Beheshti E, Tomlinson Guns E, Gleave M, Gho YS, Mathivanan S, Yang W, Freeman MR, Di Vizio D"
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - expressing DIAPH3 hRNA
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
58
Experiment ID 275
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
59
Experiment ID 274
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
60
Experiment ID 217
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
61
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for ITGAV
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ITGB1 3688
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Co-localization Homo sapiens
2 MMP2 4313
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
3 CEACAM8 1088
Affinity Capture-MS Homo sapiens
4 SHC1 6464
Affinity Capture-Western Homo sapiens
5 VDAC1 7416
Co-fractionation Homo sapiens
6 ECEL1  
Affinity Capture-MS Homo sapiens
7 PLD4  
Affinity Capture-MS Homo sapiens
8 UBC 7316
Reconstituted Complex Homo sapiens
9 LGALS3BP 3959
Reconstituted Complex Homo sapiens
10 KIAA1429 25962
Affinity Capture-MS Homo sapiens
11 NCOR1  
Affinity Capture-Western Homo sapiens
12 B3GNT2 10678
Affinity Capture-MS Homo sapiens
13 CLEC12B  
Affinity Capture-MS Homo sapiens
14 PAK4 10298
Affinity Capture-Western Homo sapiens
15 CEMIP 57214
Affinity Capture-MS Homo sapiens
16 ADAM9 8754
Affinity Capture-Western Homo sapiens
17 FBXW7  
Synthetic Lethality Homo sapiens
18 PSMD12 5718
Co-fractionation Homo sapiens
19 NCR3  
Affinity Capture-MS Homo sapiens
20 ITGA4 3676
Co-localization Homo sapiens
21 CYR61 3491
Reconstituted Complex Homo sapiens
22 ST8SIA4 7903
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 L1CAM 3897
Reconstituted Complex Homo sapiens
24 CANX 821
Affinity Capture-MS Homo sapiens
25 Eif3i 54709
Affinity Capture-MS Mus musculus
26 BTNL2  
Affinity Capture-MS Homo sapiens
27 TMEM106A  
Affinity Capture-MS Homo sapiens
28 ERBB2 2064
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
29 MAPK1 5594
Affinity Capture-Western Homo sapiens
30 TAZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 TMEM25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 ITGB3 3690
Co-crystal Structure Homo sapiens
Co-localization Homo sapiens
33 DPEP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 MAPK3 5595
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
35 FUT8 2530
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 ST3GAL1 6482
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 NOV 4856
Reconstituted Complex Homo sapiens
38 NCOR2  
Affinity Capture-Western Homo sapiens
39 MCAM 4162
Proximity Label-MS Homo sapiens
40 CBL 867
Affinity Capture-Western Homo sapiens
41 SPAG11A  
Affinity Capture-MS Homo sapiens
42 LCN9  
Affinity Capture-MS Homo sapiens
43 NTRK1 4914
Affinity Capture-MS Homo sapiens
44 C1orf54  
Affinity Capture-MS Homo sapiens
45 DHH  
Affinity Capture-MS Homo sapiens
46 TCTN2  
Affinity Capture-MS Homo sapiens
47 THBS1 7057
Affinity Capture-Western Homo sapiens
48 BRICD5  
Affinity Capture-MS Homo sapiens
49 CD160  
Affinity Capture-MS Homo sapiens
50 VDAC2 7417
Co-fractionation Homo sapiens
51 LY86  
Affinity Capture-MS Homo sapiens
52 CLEC4F  
Affinity Capture-MS Homo sapiens
53 KRT3 3850
Co-fractionation Homo sapiens
54 DEFA1 1667
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 RAB7A 7879
Proximity Label-MS Homo sapiens
56 LGALS1 3956
Affinity Capture-MS Homo sapiens
57 C1QA 712
Affinity Capture-MS Homo sapiens
58 GGH 8836
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 B3GNT3 10331
Affinity Capture-MS Homo sapiens
60 PRG2  
Affinity Capture-MS Homo sapiens
61 GAA 2548
Affinity Capture-MS Homo sapiens
62 FBXO6 26270
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 INSL5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 FN1 2335
Affinity Capture-MS Homo sapiens
65 DEFB136  
Affinity Capture-MS Homo sapiens
66 CUL9  
Affinity Capture-MS Homo sapiens
67 STAT1 6772
Affinity Capture-Western Homo sapiens
68 PTK2 5747
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
69 ATP2A1 487
Proximity Label-MS Homo sapiens
70 FBXO2 26232
Affinity Capture-MS Homo sapiens
71 PSCA 8000
Affinity Capture-MS Homo sapiens
72 PLA2G2A 5320
Reconstituted Complex Homo sapiens
73 CST9L  
Affinity Capture-MS Homo sapiens
74 ILK 3611
Affinity Capture-Western Homo sapiens
75 TNFSF13B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 ANGPT2 285
Affinity Capture-Western Homo sapiens
77 CSF1R  
Affinity Capture-Western Homo sapiens
78 LYZL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 EDIL3 10085
Affinity Capture-MS Homo sapiens
80 FCGR3B  
Affinity Capture-MS Homo sapiens
81 NAAA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 VDAC3 7419
Co-fractionation Homo sapiens
83 PLP1 5354
Affinity Capture-Western Homo sapiens
84 MCAT 27349
Co-fractionation Homo sapiens
85 SPP1 6696
Reconstituted Complex Homo sapiens
86 Itgb1 16412
Affinity Capture-MS Mus musculus
87 PTCH1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 AGMAT  
Affinity Capture-MS Homo sapiens
89 LY6G5C  
Affinity Capture-MS Homo sapiens
90 SFTPC  
Affinity Capture-MS Homo sapiens
91 AUP1 550
Two-hybrid Homo sapiens
92 PHKA2 5256
Affinity Capture-Western Homo sapiens
93 ITGAV 3685
Co-localization Homo sapiens
Co-localization Homo sapiens
94 PLD3 23646
Affinity Capture-MS Homo sapiens
95 NCL 4691
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
96 SUSD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 PXN 5829
Affinity Capture-Western Homo sapiens
98 EPHA2 1969
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
99 ITGB5 3693
Affinity Capture-Western Homo sapiens
100 BCAR1 9564
Affinity Capture-Western Homo sapiens
101 TRAV20  
Affinity Capture-MS Homo sapiens
102 CAV1 857
Proximity Label-MS Homo sapiens
103 MFGE8 4240
Affinity Capture-MS Homo sapiens
104 EP300 2033
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which ITGAV is involved
PathwayEvidenceSource
Adaptive Immune System TAS Reactome
Antigen processing-Cross presentation TAS Reactome
Axon guidance TAS Reactome
Cell surface interactions at the vascular wall TAS Reactome
Cellular responses to mechanical stimuli TAS Reactome
Cellular responses to stimuli TAS Reactome
Class I MHC mediated antigen processing & presentation TAS Reactome
Cross-presentation of particulate exogenous antigens (phagosomes) TAS Reactome
Developmental Biology TAS Reactome
ECM proteoglycans TAS Reactome
Elastic fibre formation TAS Reactome
Extracellular matrix organization TAS Reactome
Extracellular matrix organization IEA Reactome
Hemostasis TAS Reactome
Immune System TAS Reactome
Innate Immune System TAS Reactome
Integrin cell surface interactions TAS Reactome
Integrin cell surface interactions IEA Reactome
L1CAM interactions TAS Reactome
Laminin interactions IEA Reactome
Molecules associated with elastic fibres TAS Reactome
Nervous system development TAS Reactome
Neutrophil degranulation TAS Reactome
Non-integrin membrane-ECM interactions IEA Reactome
Non-integrin membrane-ECM interactions TAS Reactome
PECAM1 interactions TAS Reactome
Response of endothelial cells to shear stress TAS Reactome
Signal Transduction TAS Reactome
Signal transduction by L1 TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by TGF-beta Receptor Complex TAS Reactome
Signaling by TGFB family members TAS Reactome
Signaling by VEGF TAS Reactome
Syndecan interactions IEA Reactome
Syndecan interactions TAS Reactome
TGF-beta receptor signaling activates SMADs TAS Reactome
Turbulent (oscillatory, disturbed) flow shear stress activates signaling by PIEZO1 and integrins in endothelial cells TAS Reactome
VEGFA-VEGFR2 Pathway TAS Reactome





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