Gene description for ITGA4
Gene name integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
Gene symbol ITGA4
Other names/aliases CD49D
IA4
Species Homo sapiens
 Database cross references - ITGA4
ExoCarta ExoCarta_3676
Vesiclepedia VP_3676
Entrez Gene 3676
HGNC 6140
MIM 192975
UniProt P13612  
 ITGA4 identified in exosomes derived from the following tissue/cell type
B cells 12519789    
B cells 20458337    
B cells 20458337    
B cells 20458337    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for ITGA4
Molecular Function
    fibronectin binding GO:0001968 IEA
    integrin binding GO:0005178 IBA
    integrin binding GO:0005178 TAS
    protein binding GO:0005515 IPI
    coreceptor activity GO:0015026 TAS
    C-X3-C chemokine binding GO:0019960 IDA
    metal ion binding GO:0046872 IEA
    cell adhesion molecule binding GO:0050839 IMP
    cell adhesion molecule binding GO:0050839 IPI
    protein antigen binding GO:1990405 IEA
Biological Process
    immune response in gut-associated lymphoid tissue GO:0002387 NAS
    cell-matrix adhesion involved in ameboidal cell migration GO:0003366 IMP
    leukocyte cell-cell adhesion GO:0007159 IDA
    leukocyte cell-cell adhesion GO:0007159 NAS
    cell-matrix adhesion GO:0007160 IBA
    cell-matrix adhesion GO:0007160 IDA
    cell-matrix adhesion GO:0007160 IMP
    cell-matrix adhesion GO:0007160 NAS
    integrin-mediated signaling pathway GO:0007229 IBA
    integrin-mediated signaling pathway GO:0007229 NAS
    B cell differentiation GO:0030183 IC
    cell adhesion mediated by integrin GO:0033627 IBA
    cell-cell adhesion mediated by integrin GO:0033631 IGI
    heterotypic cell-cell adhesion GO:0034113 IGI
    heterotypic cell-cell adhesion GO:0034113 IMP
    substrate adhesion-dependent cell spreading GO:0034446 IMP
    endodermal cell differentiation GO:0035987 IEP
    receptor clustering GO:0043113 IMP
    negative regulation of vasoconstriction GO:0045906 IMP
    leukocyte tethering or rolling GO:0050901 IMP
    diapedesis GO:0050904 IEA
    axonogenesis involved in innervation GO:0060385 IEA
    cellular response to cytokine stimulus GO:0071345 IEA
    negative regulation of protein homodimerization activity GO:0090074 IDA
    cell-cell adhesion GO:0098609 IBA
    positive regulation of leukocyte tethering or rolling GO:1903238 IEA
    cellular response to amyloid-beta GO:1904646 IGI
    positive regulation of vascular endothelial cell proliferation GO:1905564 IMP
    neuron projection extension GO:1990138 IEA
    clathrin-dependent extracellular exosome endocytosis GO:1990771 IEA
    positive regulation of endothelial cell apoptotic process GO:2000353 IMP
    positive regulation of T cell migration GO:2000406 IEA
Subcellular Localization
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    focal adhesion GO:0005925 HDA
    integrin complex GO:0008305 IBA
    external side of plasma membrane GO:0009897 IBA
    cell surface GO:0009986 IDA
    membrane GO:0016020 HDA
    growth cone GO:0030426 IEA
    integrin alpha4-beta1 complex GO:0034668 IPI
    integrin alpha4-beta1 complex GO:0034668 TAS
    integrin alpha4-beta7 complex GO:0034669 IDA
    integrin alpha4-beta7 complex GO:0034669 IPI
    neuronal cell body GO:0043025 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified ITGA4 in exosomes
1
Experiment ID 11
MISEV standards
✘ Biophysical techniques
✔
HSC70|HSP90|MHCII|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
PubMed ID 12519789    
Organism Homo sapiens
Experiment description Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation.
Authors "Wubbolts R, Leckie RS, Veenhuizen PT, Schwarzmann G, Mobius W, Hoernschemeyer J, Slot JW, Geuze HJ, Stoorvogel W"
Journal name JBC
Publication year 2003
Sample B cells
Sample name RN (HLA-DR15+)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
Thin layer chromatography
HPLC
2
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
5
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
16
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
17
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
19
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
20
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
21
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
22
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ITGA4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF3A 8661
Affinity Capture-MS Homo sapiens
2 HIST1H4K 8362
Affinity Capture-MS Homo sapiens
3 KRT1 3848
Affinity Capture-MS Homo sapiens
4 PRPF8 10594
Affinity Capture-MS Homo sapiens
5 PKM 5315
Affinity Capture-MS Homo sapiens
6 KRT6A 3853
Affinity Capture-MS Homo sapiens
7 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
8 DDX17 10521
Affinity Capture-MS Homo sapiens
9 ACTC1 70
Affinity Capture-MS Homo sapiens
10 HIST1H2BH 8345
Affinity Capture-MS Homo sapiens
11 SLC2A1 6513
Affinity Capture-MS Homo sapiens
12 UBC 7316
Affinity Capture-MS Homo sapiens
13 CSE1L 1434
Affinity Capture-MS Homo sapiens
14 NHP2L1 4809
Affinity Capture-MS Homo sapiens
15 GCN1L1 10985
Affinity Capture-MS Homo sapiens
16 PRKDC 5591
Affinity Capture-MS Homo sapiens
17 DECR1 1666
Affinity Capture-MS Homo sapiens
18 SNRNP70 6625
Affinity Capture-MS Homo sapiens
19 PFKL 5211
Affinity Capture-MS Homo sapiens
20 DARS 1615
Affinity Capture-MS Homo sapiens
21 HIST3H2A 92815
Affinity Capture-MS Homo sapiens
22 AP2A1 160
Affinity Capture-MS Homo sapiens
23 HYOU1 10525
Affinity Capture-MS Homo sapiens
24 DRG1 4733
Affinity Capture-MS Homo sapiens
25 CPSF7 79869
Affinity Capture-MS Homo sapiens
26 PSMB6 5694
Affinity Capture-MS Homo sapiens
27 EPS15 2060
Affinity Capture-MS Homo sapiens
28 RPS9 6203
Affinity Capture-MS Homo sapiens
29 EIF3E 3646
Affinity Capture-MS Homo sapiens
30 SNRPD3 6634
Affinity Capture-MS Homo sapiens
31 UBE2NL 389898
Affinity Capture-MS Homo sapiens
32 RPS11 6205
Affinity Capture-MS Homo sapiens
33 TFRC 7037
Affinity Capture-MS Homo sapiens
34 EEF1A1 1915
Affinity Capture-MS Homo sapiens
35 RBM14 10432
Affinity Capture-MS Homo sapiens
36 MYL6 4637
Affinity Capture-MS Homo sapiens
37 RBMX 27316
Affinity Capture-MS Homo sapiens
38 LDHB 3945
Affinity Capture-MS Homo sapiens
39 TCP1 6950
Affinity Capture-MS Homo sapiens
40 CAPZA1 829
Affinity Capture-MS Homo sapiens
41 PELO 53918
Cross-Linking-MS (XL-MS) Homo sapiens
42 ANKRD17 26057
Affinity Capture-MS Homo sapiens
43 HIST1H3C 8352
Affinity Capture-MS Homo sapiens
44 HIST1H2AG 8969
Affinity Capture-MS Homo sapiens
45 MAPRE1 22919
Affinity Capture-MS Homo sapiens
46 MCAM 4162
Proximity Label-MS Homo sapiens
47 PPIH 10465
Affinity Capture-MS Homo sapiens
48 PABPC1 26986
Affinity Capture-MS Homo sapiens
49 EEF1E1 9521
Affinity Capture-MS Homo sapiens
50 PPP2CB 5516
Affinity Capture-MS Homo sapiens
51 PSMD2 5708
Affinity Capture-MS Homo sapiens
52 MYH9 4627
Affinity Capture-MS Homo sapiens
53 ACTN4 81
Affinity Capture-MS Homo sapiens
54 KRT9 3857
Affinity Capture-MS Homo sapiens
55 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
56 CD81 975
Affinity Capture-Western Homo sapiens
57 HIST1H1C 3006
Affinity Capture-MS Homo sapiens
58 ENO1 2023
Affinity Capture-MS Homo sapiens
59 SNRPD1 6632
Affinity Capture-MS Homo sapiens
60 CAPN1 823
Affinity Capture-MS Homo sapiens
61 DDX39B 7919
Affinity Capture-MS Homo sapiens
62 PICALM 8301
Affinity Capture-MS Homo sapiens
63 VDAC2 7417
Affinity Capture-MS Homo sapiens
64 SEC61B 10952
Affinity Capture-MS Homo sapiens
65 SLC25A5 292
Affinity Capture-MS Homo sapiens
66 HIST1H4C 8364
Affinity Capture-MS Homo sapiens
67 CNBP 7555
Affinity Capture-MS Homo sapiens
68 HIST1H2BL 8340
Affinity Capture-MS Homo sapiens
69 HIST1H3F 8968
Affinity Capture-MS Homo sapiens
70 VAMP8 8673
Affinity Capture-MS Homo sapiens
71 LRRC59 55379
Affinity Capture-MS Homo sapiens
72 SDHA 6389
Affinity Capture-MS Homo sapiens
73 TYMS 7298
Affinity Capture-MS Homo sapiens
74 RAP1B 5908
Affinity Capture-MS Homo sapiens
75 HSPE1 3336
Affinity Capture-MS Homo sapiens
76 PON2 5445
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 ANXA1 301
Affinity Capture-MS Homo sapiens
78 YWHAZ 7534
Affinity Capture-MS Homo sapiens
79 ARCN1 372
Affinity Capture-MS Homo sapiens
80 EIF4E 1977
Affinity Capture-MS Homo sapiens
81 RPL35 11224
Affinity Capture-MS Homo sapiens
82 KPNA2 3838
Affinity Capture-MS Homo sapiens
83 FN1 2335
Affinity Capture-MS Homo sapiens
84 HIST1H4B 8366
Affinity Capture-MS Homo sapiens
85 PCBP1 5093
Affinity Capture-MS Homo sapiens
86 LSM3 27258
Affinity Capture-MS Homo sapiens
87 GSTK1 373156
Affinity Capture-MS Homo sapiens
88 HSPA1A 3303
Affinity Capture-MS Homo sapiens
89 STMN1 3925
Affinity Capture-MS Homo sapiens
90 HIST1H4I 8294
Affinity Capture-MS Homo sapiens
91 LGALS8 3964
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 HEATR1 55127
Affinity Capture-MS Homo sapiens
93 KRT19 3880
Affinity Capture-MS Homo sapiens
94 EDDM3B  
Affinity Capture-MS Homo sapiens
95 GRB2 2885
Affinity Capture-MS Homo sapiens
96 VASP 7408
Affinity Capture-MS Homo sapiens
97 RPS16 6217
Affinity Capture-MS Homo sapiens
98 HK2 3099
Affinity Capture-MS Homo sapiens
99 PSMB2 5690
Affinity Capture-MS Homo sapiens
100 YBX1 4904
Affinity Capture-MS Homo sapiens
101 NUDT21 11051
Affinity Capture-MS Homo sapiens
102 RPS27A 6233
Affinity Capture-MS Homo sapiens
103 NAAA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 TIA1 7072
Affinity Capture-MS Homo sapiens
105 HIST1H2AC 8334
Affinity Capture-MS Homo sapiens
106 RPLP0 6175
Affinity Capture-MS Homo sapiens
107 PLEC 5339
Affinity Capture-MS Homo sapiens
108 IGHD  
Affinity Capture-MS Homo sapiens
109 EIF3I 8668