Gene description for NHP2L1
Gene name NHP2 non-histone chromosome protein 2-like 1 (S. cerevisiae)
Gene symbol NHP2L1
Other names/aliases 15.5K
FA-1
FA1
NHPX
OTK27
SNRNP15-5
SNU13
SPAG12
SSFA1
Species Homo sapiens
 Database cross references - NHP2L1
ExoCarta ExoCarta_4809
Entrez Gene 4809
HGNC 7819
MIM 601304
UniProt P55769  
 NHP2L1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for NHP2L1
Molecular Function
    protein binding GO:0005515 IPI
    U4 snRNA binding GO:0030621 IDA
    box C/D snoRNA binding GO:0034512 IDA
    snoRNA binding GO:0030515 IDA
    U3 snoRNA binding GO:0034511 IDA
    poly(A) RNA binding GO:0044822 IDA
    RNA binding GO:0003723 TAS
Biological Process
    ribosome biogenesis GO:0042254 IEA
    RNA splicing GO:0008380 TAS
    mRNA splicing, via spliceosome GO:0000398 TAS
    gene expression GO:0010467 TAS
Subcellular Localization
    nucleolus GO:0005730 TAS
    protein complex GO:0043234 IDA
    nucleus GO:0005634 TAS
    spliceosomal complex GO:0005681 IEA
    nucleoplasm GO:0005654 TAS
    box C/D snoRNP complex GO:0031428 NAS
 Experiment description of studies that identified NHP2L1 in exosomes
1
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NHP2L1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 XRCC6 2547
Two-hybrid Homo sapiens
2 POLA2  
Two-hybrid Homo sapiens
3 GTF3C1  
Two-hybrid Homo sapiens
4 RBM10  
Two-hybrid Homo sapiens
5 RAD17  
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
6 CDKN2C  
Two-hybrid Homo sapiens
7 RIF1  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which NHP2L1 is involved
PathwayEvidenceSource
mRNA Splicing - Major Pathway TAS Reactome
mRNA Splicing - Minor Pathway TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here