Gene description for HSD17B10
Gene name hydroxysteroid (17-beta) dehydrogenase 10
Gene symbol HSD17B10
Other names/aliases 17b-HSD10
ABAD
CAMR
DUPXp11.22
ERAB
HADH2
HCD2
MHBD
MRPP2
MRX17
MRX31
MRXS10
SCHAD
SDR5C1
Species Homo sapiens
 Database cross references - HSD17B10
ExoCarta ExoCarta_3028
Entrez Gene 3028
HGNC 4800
MIM 300256
UniProt Q99714  
 HSD17B10 identified in exosomes derived from the following tissue/cell type
Breast cancer cells 19415654    
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for HSD17B10
Molecular Function
    3-hydroxyacyl-CoA dehydrogenase activity GO:0003857 EXP
    testosterone dehydrogenase [NAD(P)] activity GO:0030283 IEA
    cholate 7-alpha-dehydrogenase activity GO:0008709 TAS
    protein binding GO:0005515 IPI
    3-hydroxy-2-methylbutyryl-CoA dehydrogenase activity GO:0047015 IEA
    poly(A) RNA binding GO:0044822 IDA
Biological Process
    oxidation-reduction process GO:0055114 IEA
    lipid metabolic process GO:0006629 TAS
    branched-chain amino acid catabolic process GO:0009083 TAS
    tRNA processing GO:0008033 IEA
    small molecule metabolic process GO:0044281 TAS
    cellular nitrogen compound metabolic process GO:0034641 TAS
Subcellular Localization
    endoplasmic reticulum GO:0005783 IEA
    mitochondrion GO:0005739 ISS
    mitochondrial matrix GO:0005759 TAS
    mitochondrial inner membrane GO:0005743 IEA
    cytoplasm GO:0005737 TAS
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified HSD17B10 in exosomes
1
Experiment ID 65
ISEV standards
EV Biophysical techniques
HSP60|HSP27|GAPDH
EV Cytosolic markers
FLOT2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19415654    
Organism Homo sapiens
Experiment description Proteomics of MUC1-containing lipid rafts from plasma membranes and exosomes of human breast carcinoma cells MCF-7.
Authors Staubach S, Razawi H, Hanisch FG.
Journal name PROTEOMICS
Publication year 2009
Sample Breast cancer cells
Sample name MCF-7
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
2
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HSD17B10
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APP 351
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which HSD17B10 is involved
PathwayEvidenceSource
Branched-chain amino acid catabolism TAS Reactome





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