Gene description for Pygb
Gene name brain glycogen phosphorylase
Gene symbol Pygb
Other names/aliases -
Species Mus musculus
 Database cross references - Pygb
ExoCarta ExoCarta_110078
Vesiclepedia VP_110078
Entrez Gene 110078
UniProt Q8CI94  
 Pygb identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pygb
Molecular Function
    phosphorylase activity GO:0004645 IEA
    transferase activity GO:0016740 IEA
    glycogen phosphorylase activity GO:0008184 ISO
    catalytic activity GO:0003824 IEA
    drug binding GO:0008144 ISO
    transferase activity, transferring glycosyl groups GO:0016757 IEA
    pyridoxal phosphate binding GO:0030170 IBA
    carbohydrate binding GO:0030246 ISO
    protein homodimerization activity GO:0042803 ISO
Biological Process
    carbohydrate metabolic process GO:0005975 IEA
    glycogen catabolic process GO:0005980 ISO
    glycogen metabolic process GO:0005977 ISO
    metabolic process GO:0008152 IEA
Subcellular Localization
    cytoplasm GO:0005737 ISO
    membrane GO:0016020 ISO
    extracellular exosome GO:0070062 ISO
    axon GO:0030424 ISO
 Experiment description of studies that identified Pygb in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pygb
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Pygb is involved
PathwayEvidenceSource
Glycogen breakdown (glycogenolysis) IEA Reactome





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