Gene description for Pygl
Gene name liver glycogen phosphorylase
Gene symbol Pygl
Other names/aliases -
Species Mus musculus
 Database cross references - Pygl
ExoCarta ExoCarta_110095
Vesiclepedia VP_110095
Entrez Gene 110095
UniProt Q9ET01  
 Pygl identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pygl
Molecular Function
    ATP binding GO:0005524 ISO
    AMP binding GO:0016208 ISO
    protein homodimerization activity GO:0042803 ISO
    catalytic activity GO:0003824 IEA
    carbohydrate binding GO:0030246 ISO
    pyridoxal phosphate binding GO:0030170 IBA
    drug binding GO:0008144 ISO
    purine nucleobase binding GO:0002060 ISO
    bile acid binding GO:0032052 ISO
    phosphorylase activity GO:0004645 IEA
    nucleotide binding GO:0000166 IEA
    vitamin binding GO:0019842 ISO
    protein binding GO:0005515 IPI
    transferase activity, transferring glycosyl groups GO:0016757 IEA
    transferase activity GO:0016740 IEA
    glycogen phosphorylase activity GO:0008184 ISO
Biological Process
    metabolic process GO:0008152 IEA
    necroptotic process GO:0070266 IGI
    glycogen metabolic process GO:0005977 ISO
    5-phosphoribose 1-diphosphate biosynthetic process GO:0006015 ISO
    glycogen catabolic process GO:0005980 ISO
    carbohydrate metabolic process GO:0005975 IEA
    glucose homeostasis GO:0042593 ISO
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    plasma membrane GO:0005886 ISO
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Pygl in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pygl
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Pygl is involved
PathwayEvidenceSource
Glycogen breakdown (glycogenolysis) IEA Reactome





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