Gene description for Tkt
Gene name transketolase
Gene symbol Tkt
Other names/aliases p68
Species Mus musculus
 Database cross references - Tkt
ExoCarta ExoCarta_21881
Entrez Gene 21881
UniProt P40142  
 Tkt identified in exosomes derived from the following tissue/cell type
Oligodendrocytes 21136642    
 Gene ontology annotations for Tkt
Molecular Function
    catalytic activity GO:0003824 IEA
    monosaccharide binding GO:0048029 ISO
    thiamine pyrophosphate binding GO:0030976 ISO
    cofactor binding GO:0048037 ISO
    transferase activity GO:0016740 IEA
    metal ion binding GO:0046872 IEA
    magnesium ion binding GO:0000287 ISO
    protein homodimerization activity GO:0042803 ISO
    carbohydrate binding GO:0030246 ISO
    transketolase activity GO:0004802 ISO
Biological Process
    glyceraldehyde-3-phosphate biosynthetic process GO:0046166 ISO
    metabolic process GO:0008152 IEA
    regulation of growth GO:0040008 IMP
    pentose-phosphate shunt GO:0006098 ISO
    ribose phosphate biosynthetic process GO:0046390 ISO
    pentose-phosphate shunt, non-oxidative branch GO:0009052 ISO
Subcellular Localization
    peroxisome GO:0005777 ISO
    nucleoplasm GO:0005654 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    vesicle GO:0031982 ISO
    myelin sheath GO:0043209 IDA
    nucleus GO:0005634 ISO
    extracellular exosome GO:0070062 ISO
    endoplasmic reticulum membrane GO:0005789 ISO
 Experiment description of studies that identified Tkt in exosomes
1
Experiment ID 33
ISEV standards
EM|IEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Tkt
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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