Gene description for Uba6
Gene name ubiquitin-like modifier activating enzyme 6
Gene symbol Uba6
Other names/aliases 4930542H01
5730469D23Rik
AU021846
AW124799
E1-L2
Ube1l2
Species Mus musculus
 Database cross references - Uba6
ExoCarta ExoCarta_231380
Vesiclepedia VP_231380
Entrez Gene 231380
UniProt Q8C7R4  
 Uba6 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Uba6
Molecular Function
    ubiquitin activating enzyme activity GO:0004839 IBA
    ubiquitin activating enzyme activity GO:0004839 IMP
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    FAT10 activating enzyme activity GO:0019780 IBA
    FAT10 activating enzyme activity GO:0019780 IMP
    FAT10 activating enzyme activity GO:0019780 ISO
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    ubiquitin-dependent protein catabolic process GO:0006511 IGI
    ubiquitin-dependent protein catabolic process GO:0006511 IMP
    ubiquitin-dependent protein catabolic process GO:0006511 ISO
    DNA damage response GO:0006974 IBA
    learning GO:0007612 IMP
    locomotory behavior GO:0007626 IMP
    protein ubiquitination GO:0016567 IBA
    protein ubiquitination GO:0016567 IEA
    protein ubiquitination GO:0016567 IMP
    protein ubiquitination GO:0016567 ISO
    amygdala development GO:0021764 IMP
    hippocampus development GO:0021766 IMP
    dendritic spine development GO:0060996 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Uba6 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Uba6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Hexa 15211
Co-fractionation Mus musculus
2 Ubc  
Reconstituted Complex Mus musculus
3 Ube2l3 22195
Co-fractionation Mus musculus
4 Cyfip1 20430
Affinity Capture-MS Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Ahcyl1 229709
Co-fractionation Mus musculus
7 Twf2  
Co-fractionation Mus musculus
8 Gnpda1  
Co-fractionation Mus musculus
9 Rad23b 19359
Co-fractionation Mus musculus
10 Xpnpep1 170750
Co-fractionation Mus musculus
11 Uba1 22201
Co-fractionation Mus musculus
12 Pygb 110078
Co-fractionation Mus musculus
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