Gene description for Uba1
Gene name ubiquitin-like modifier activating enzyme 1
Gene symbol Uba1
Other names/aliases A1S9
Sbx
Ube-1
Ube1x
Species Mus musculus
 Database cross references - Uba1
ExoCarta ExoCarta_22201
Entrez Gene 22201
UniProt Q02053  
 Uba1 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Pancreatic cells 19351151    
 Gene ontology annotations for Uba1
Molecular Function
    protein binding GO:0005515 IPI
    ligase activity GO:0016874 IEA
    ubiquitin activating enzyme activity GO:0004839 ISO
    poly(A) RNA binding GO:0044822 ISO
    ubiquitin-protein transferase activity GO:0004842 IBA
    nucleotide binding GO:0000166 IEA
    ATP binding GO:0005524 IEA
    small protein activating enzyme activity GO:0008641 IEA
Biological Process
    protein ubiquitination GO:0016567 ISO
    cellular response to DNA damage stimulus GO:0006974 ISO
    cellular protein modification process GO:0006464 IEA
    modification-dependent protein catabolic process GO:0019941 IBA
Subcellular Localization
    mitochondrion GO:0005739 ISO
    extracellular exosome GO:0070062 ISO
    desmosome GO:0030057 ISO
    heterochromatin GO:0000792 ISO
    endosome membrane GO:0010008 ISO
    lysosomal membrane GO:0005765 ISO
    nucleus GO:0005634 ISO
    rough endoplasmic reticulum membrane GO:0030867 ISO
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IBA
 Experiment description of studies that identified Uba1 in exosomes
1
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 188
ISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Uba1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HSPH1 10808
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which Uba1 is involved
PathwayEvidenceSource
Antigen processing: Ubiquitination & Proteasome degradation IEA Reactome





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