Gene description for Uchl3
Gene name ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
Gene symbol Uchl3
Other names/aliases -
Species Mus musculus
 Database cross references - Uchl3
ExoCarta ExoCarta_50933
Vesiclepedia VP_50933
Entrez Gene 50933
UniProt Q9JKB1  
 Uchl3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Uchl3
Molecular Function
    cysteine-type deubiquitinase activity GO:0004843 IBA
    cysteine-type deubiquitinase activity GO:0004843 IEA
    cysteine-type deubiquitinase activity GO:0004843 ISO
    peptidase activity GO:0008233 IDA
    peptidase activity GO:0008233 ISO
    ubiquitin binding GO:0043130 ISO
    deubiquitinase activity GO:0101005 IDA
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 IEA
    adult walking behavior GO:0007628 IGI
    protein deubiquitination GO:0016579 IDA
    protein deubiquitination GO:0016579 ISA
    protein deubiquitination GO:0016579 ISO
    protein catabolic process GO:0030163 IBA
    protein catabolic process GO:0030163 IDA
    protein catabolic process GO:0030163 ISO
    cellular response to insulin stimulus GO:0032869 IDA
    eating behavior GO:0042755 IGI
    positive regulation of fat cell differentiation GO:0045600 IDA
    retina development in camera-type eye GO:0060041 IMP
Subcellular Localization
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Uchl3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Uchl3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atg5  
Affinity Capture-MS Mus musculus
2 Nedd8  
Reconstituted Complex Mus musculus
3 Uba1 22201
Co-fractionation Mus musculus
4 Ubc  
Affinity Capture-MS Mus musculus
Reconstituted Complex Mus musculus
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