ExoCarta: Gene summary

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Gene description for PCM1
Gene name	pericentriolar material 1
Gene symbol	PCM1
Other names/aliases	PTC4
Species	Homo sapiens
Database cross references - PCM1
ExoCarta	ExoCarta_5108
Vesiclepedia	VP_5108
Entrez Gene	5108
HGNC	8727
MIM	600299
UniProt	Q15154
PCM1 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells	36408942

Gene ontology annotations for PCM1

Molecular Function
	identical protein binding	GO:0042802	ISS
	protein binding	GO:0005515	IPI
Biological Process
	intraciliary transport involved in cilium morphogenesis	GO:0035735	IMP
	cytoplasmic microtubule organization	GO:0031122	IMP
	cilium assembly	GO:0042384	IMP
	mitotic cell cycle	GO:0000278	TAS
	interkinetic nuclear migration	GO:0022027	ISS
	G2/M transition of mitotic cell cycle	GO:0000086	TAS
	microtubule anchoring	GO:0034453	ISS
	nonmotile primary cilium assembly	GO:0035058	IMP
	centrosome organization	GO:0051297	IMP
	protein localization to centrosome	GO:0071539	ISS
	organelle organization	GO:0006996	TAS
	neuronal stem cell maintenance	GO:0097150	IEA
	positive regulation of intracellular protein transport	GO:0090316	IMP
	negative regulation of neurogenesis	GO:0050768	ISS
	microtubule anchoring at centrosome	GO:0034454	IEA
Subcellular Localization
	nuclear membrane	GO:0031965	IDA
	protein complex	GO:0043234	IDA
	ciliary basal body	GO:0036064	IEA
	nonmotile primary cilium	GO:0031513	IDA
	cytosol	GO:0005829	TAS
	cytoplasm	GO:0005737	ISS
	membrane	GO:0016020	IDA
	pericentriolar material	GO:0000242	ISS
	centriolar satellite	GO:0034451	IDA
	centrosome	GO:0005813	ISS

Experiment description of studies that identified PCM1 in exosomes

Experiment ID

488

MISEV standards
✔ EM	EV Biophysical techniques
✔ CD9\|CD81\|CD63\|GAPDH\|SDCBP\|LAMP1\|TFRC\|UCHL1\|FLOT2\|LAMP2\|FLOT1\|ICAM1\|RAB5B\|CD151\|RAB35\|TSG101\|RAB5A\|CD82	EV Enriched markers
✔ CANX	EV Negative markers
✔ NTA	EV Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Homo sapiens

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.

Journal name

Proteomics

Publication year

2023

Sample

Mesenchymal stem cells

Sample name

UCMSC

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Protein-protein interactions for PCM1

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

PCNT2

Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens

HAP1

Invitro		Homo sapiens
Two-hybrid		Homo sapiens
Invivo		Homo sapiens

Cep72

Two-hybrid

Homo sapiens

KRT15

3866

Two-hybrid

Homo sapiens

BBS4

585

Two-hybrid		Homo sapiens
Affinity Capture-MS		Homo sapiens

KIAA0368

23392

Two-hybrid

Homo sapiens

ABI2

10152

Two-hybrid

Homo sapiens

KRT19

3880

Two-hybrid

Homo sapiens

EPS8

2059

Two-hybrid

Homo sapiens

EXOC8

149371

Two-hybrid

Homo sapiens

ING5

Two-hybrid

Homo sapiens

CCDC53

51019

Two-hybrid

Homo sapiens

TTC8

123016

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which PCM1 is involved

Pathway	Evidence	Source
Anchoring of the basal body to the plasma membrane	TAS	Reactome
Loss of Nlp from mitotic centrosomes	TAS	Reactome
Loss of proteins required for interphase microtubule organization from the centrosome	TAS	Reactome
Recruitment of mitotic centrosome proteins and complexes	TAS	Reactome
Regulation of PLK1 Activity at G2/M Transition	TAS	Reactome

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