Gene description for TSC1
Gene name tuberous sclerosis 1
Gene symbol TSC1
Other names/aliases LAM
TSC
Species Homo sapiens
 Database cross references - TSC1
ExoCarta ExoCarta_7248
Vesiclepedia VP_7248
Entrez Gene 7248
HGNC 12362
MIM 605284
UniProt Q92574  
 TSC1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for TSC1
Molecular Function
    chaperone binding GO:0051087 IPI
    protein binding GO:0005515 IPI
    GTPase activating protein binding GO:0032794 IEA
    GTPase regulator activity GO:0030695 IBA
    protein N-terminus binding GO:0047485 IPI
Biological Process
    response to insulin GO:0032868 IDA
    myelination GO:0042552 IEA
    potassium ion transport GO:0006813 IEA
    cell-matrix adhesion GO:0007160 IMP
    regulation of phosphoprotein phosphatase activity GO:0043666 IMP
    hippocampus development GO:0021766 IEA
    protein heterooligomerization GO:0051291 IEA
    gene expression GO:0010467 TAS
    regulation of stress fiber assembly GO:0051492 IDA
    glucose import GO:0046323 IEA
    cerebral cortex development GO:0021987 IEA
    negative regulation of cell proliferation GO:0008285 IMP
    cardiac muscle cell differentiation GO:0055007 IEA
    negative regulation of insulin receptor signaling pathway GO:0046627 IBA
    positive regulation of focal adhesion assembly GO:0051894 IDA
    negative regulation of cell size GO:0045792 IEA
    insulin receptor signaling pathway GO:0008286 TAS
    rRNA export from nucleus GO:0006407 IMP
    protein stabilization GO:0050821 IDA
    negative regulation of TOR signaling GO:0032007 IMP
    kidney development GO:0001822 IEA
    negative regulation of translation GO:0017148 IMP
    cell projection organization GO:0030030 IEA
    regulation of cell cycle GO:0051726 IBA
    synapse organization GO:0050808 IEA
    regulation of protein kinase activity GO:0045859 IEA
    activation of GTPase activity GO:0090630 IDA
    regulation of translation GO:0006417 IDA
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    regulation of cell-matrix adhesion GO:0001952 IMP
    neural tube closure GO:0001843 IEA
    cell cycle arrest GO:0007050 TAS
Subcellular Localization
    cell cortex GO:0005938 IDA
    membrane GO:0016020 IDA
    growth cone GO:0030426 IEA
    lamellipodium GO:0030027 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 TAS
    actin filament GO:0005884 IDA
    intracellular membrane-bounded organelle GO:0043231 IEA
    TSC1-TSC2 complex GO:0033596 IDA
    protein complex GO:0043234 IDA
 Experiment description of studies that identified TSC1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TSC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tsc2 24855
Co-localization Rattus norvegicus
2 GSK3B 2932
Affinity Capture-Western Homo sapiens
3 TSC2 7249
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
4 CDKN1B 1027
Affinity Capture-Western Homo sapiens
5 DOCK7 85440
Affinity Capture-MS Homo sapiens
6 AXIN1  
Affinity Capture-Western Homo sapiens
7 NF2 4771
Invivo Homo sapiens
8 YWHAA  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 EZR 7430
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
10 YWHAZ 7534
Two-hybrid Homo sapiens
11 PTK2 5747
Affinity Capture-Western Homo sapiens
12 CDK1 983
Reconstituted Complex Homo sapiens
13 ACTA1 58
Co-localization Homo sapiens
14 RHEB 6009
Biochemical Activity Homo sapiens
15 RDX 5962
Invitro Homo sapiens
Two-hybrid Homo sapiens
16 NEFL 4747
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
17 PAM 5066
Co-localization Homo sapiens
18 PLK1 5347
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
19 MSN 4478
Two-hybrid Homo sapiens
20 AKT1 207
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 TSC1 7248
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
22 RPS6KA1 6195
Affinity Capture-Western Homo sapiens
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