Gene description for TSC1
Gene name tuberous sclerosis 1
Gene symbol TSC1
Other names/aliases LAM
TSC
Species Homo sapiens
 Database cross references - TSC1
ExoCarta ExoCarta_7248
Vesiclepedia VP_7248
Entrez Gene 7248
HGNC 12362
MIM 605284
UniProt Q92574  
 TSC1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for TSC1
Molecular Function
    protein binding GO:0005515 IPI
    Hsp70 protein binding GO:0030544 IDA
    ATPase inhibitor activity GO:0042030 IDA
    protein folding chaperone GO:0044183 IDA
    protein-folding chaperone binding GO:0051087 IPI
    Hsp90 protein binding GO:0051879 IPI
Biological Process
    kidney development GO:0001822 IEA
    neural tube closure GO:0001843 IEA
    regulation of cell-matrix adhesion GO:0001952 IMP
    adaptive immune response GO:0002250 IEA
    protein folding GO:0006457 IEA
    potassium ion transport GO:0006813 IEA
    cell-matrix adhesion GO:0007160 IMP
    cell population proliferation GO:0008283 IEA
    negative regulation of cell population proliferation GO:0008285 IBA
    negative regulation of cell population proliferation GO:0008285 IMP
    associative learning GO:0008306 IEA
    adult locomotory behavior GO:0008344 ISS
    cellular response to starvation GO:0009267 IDA
    negative regulation of macroautophagy GO:0016242 ISS
    hippocampus development GO:0021766 IEA
    cerebral cortex development GO:0021987 IEA
    cell projection organization GO:0030030 IEA
    negative regulation of TOR signaling GO:0032007 IBA
    negative regulation of TOR signaling GO:0032007 IDA
    negative regulation of TOR signaling GO:0032007 IMP
    negative regulation of TOR signaling GO:0032007 ISS
    negative regulation of ATP-dependent activity GO:0032780 IDA
    response to insulin GO:0032868 IDA
    cellular response to decreased oxygen levels GO:0036294 ISS
    myelination GO:0042552 IEA
    memory T cell differentiation GO:0043379 IEA
    negative regulation of cell size GO:0045792 IEA
    D-glucose import GO:0046323 IEA
    synapse organization GO:0050808 IEA
    protein stabilization GO:0050821 IDA
    protein stabilization GO:0050821 IMP
    regulation of stress fiber assembly GO:0051492 IDA
    regulation of cell cycle GO:0051726 IBA
    positive regulation of focal adhesion assembly GO:0051894 IDA
    cardiac muscle cell differentiation GO:0055007 IEA
    activation of GTPase activity GO:0090630 IDA
    negative regulation of TORC1 signaling GO:1904262 IDA
Subcellular Localization
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IDA
    lysosomal membrane GO:0005765 IDA
    lipid droplet GO:0005811 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    actin filament GO:0005884 IDA
    plasma membrane GO:0005886 TAS
    cell cortex GO:0005938 IDA
    postsynaptic density GO:0014069 IEA
    membrane GO:0016020 IDA
    lamellipodium GO:0030027 IDA
    protein-containing complex GO:0032991 IDA
    TSC1-TSC2 complex GO:0033596 EXP
    TSC1-TSC2 complex GO:0033596 IBA
    TSC1-TSC2 complex GO:0033596 IDA
    TSC1-TSC2 complex GO:0033596 IPI
    perinuclear region of cytoplasm GO:0048471 ISS
    protein folding chaperone complex GO:0101031 IDA
 Experiment description of studies that identified TSC1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TSC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SAMD11  
Two-hybrid Homo sapiens
2 ENKD1  
Two-hybrid Homo sapiens
3 RBPMS 11030
Two-hybrid Homo sapiens
4 LAMP3  
Proximity Label-MS Homo sapiens
5 AES 166
Two-hybrid Homo sapiens
6 TSC2 7249
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
7 YWHAE 7531
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 PRMT6  
Two-hybrid Homo sapiens
9 TCF7L2  
Two-hybrid Homo sapiens
10 LAMP2 3920
Proximity Label-MS Homo sapiens
11 ZIC1  
Two-hybrid Homo sapiens
12 TMED5 50999
Affinity Capture-MS Homo sapiens
13 URI1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 Tbc1d7  
Affinity Capture-MS Mus musculus
15 LMO2  
Two-hybrid Homo sapiens
16 RHEB 6009
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
17 FBXW7  
Affinity Capture-MS Homo sapiens
18 SMG9 56006
Two-hybrid Homo sapiens
19 DAPK1 1612
Affinity Capture-Western Homo sapiens
20 DYRK1A 1859
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 HR  
Two-hybrid Homo sapiens
22 CHCHD2  
Two-hybrid Homo sapiens
23 LENG1  
Two-hybrid Homo sapiens
24 PPP1R18 170954
Two-hybrid Homo sapiens
25 TBX6  
Two-hybrid Homo sapiens
26 PRC1 9055
Affinity Capture-MS Homo sapiens
27 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 AXIN1  
Affinity Capture-Western Homo sapiens
29 STX6 10228
Proximity Label-MS Homo sapiens
30 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 IGFN1  
Two-hybrid Homo sapiens
32 PCM1 5108
Affinity Capture-MS Homo sapiens
33 ARID5A  
Two-hybrid Homo sapiens
34 MAPK14 1432
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
35 AKTIP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 Tsc2 24855
Co-localization Rattus norvegicus
37 FBXW5  
Affinity Capture-Western Homo sapiens
38 GSK3B 2932
Affinity Capture-Western Homo sapiens
39 LAMTOR1 55004
Proximity Label-MS Homo sapiens
40 AKT1 207
Affinity Capture-Western Homo sapiens
41 YWHAB 7529
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 NUP155 9631
Proximity Label-MS Homo sapiens
43 PFN1 5216
Proximity Label-MS Homo sapiens
44 PAM 5066
Co-localization Homo sapiens
45 CCL28 56477
Two-hybrid Homo sapiens
46 FAM222B  
Two-hybrid Homo sapiens
47 VEZF1  
Two-hybrid Homo sapiens
48 MTCH2 23788
Proximity Label-MS Homo sapiens
49 TSGA10IP  
Two-hybrid Homo sapiens
50 MYOZ3  
Two-hybrid Homo sapiens
51 GLIS2  
Two-hybrid Homo sapiens
52 FAM110A  
Two-hybrid Homo sapiens
53 CSTF2 1478
Two-hybrid Homo sapiens
54 RAB4A 5867
Proximity Label-MS Homo sapiens
55 CPVL 54504
Affinity Capture-MS Homo sapiens
56 POU6F2  
Two-hybrid Homo sapiens
57 POGZ 23126
Two-hybrid Homo sapiens
58 RIN1 9610
Two-hybrid Homo sapiens
59 RAB2A 5862
Proximity Label-MS Homo sapiens
60 PATL1 219988
Two-hybrid Homo sapiens
61 YWHAQ 10971
Affinity Capture-MS Homo sapiens
62 VENTX  
Two-hybrid Homo sapiens
63 RPS6KA1 6195
Affinity Capture-Western Homo sapiens
64 SUOX  
Two-hybrid Homo sapiens
65 BAG3 9531
Two-hybrid Homo sapiens
66 ATXN1 6310
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
67 FOXH1  
Two-hybrid Homo sapiens
68 SPAG8  
Two-hybrid Homo sapiens
69 WHAMMP3  
Affinity Capture-MS Homo sapiens
70 RRAGA 10670
Affinity Capture-Western Homo sapiens
71 CIAO1 9391
Affinity Capture-MS Homo sapiens
72 RAB5A 5868
Proximity Label-MS Homo sapiens
73 DOCK7 85440
Affinity Capture-MS Homo sapiens
74 CCDC120  
Two-hybrid Homo sapiens
75 NPTN 27020
Affinity Capture-MS Homo sapiens
76 SREBF1 6720
Negative Genetic Homo sapiens
77 CUL3 8452
Affinity Capture-MS Homo sapiens
78 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
79 FRS3 10817
Two-hybrid Homo sapiens
80 PTK2 5747
Affinity Capture-Western Homo sapiens
81 VHL  
Negative Genetic Homo sapiens
82 KRT27 342574
Affinity Capture-MS Homo sapiens
83 NXF1 10482
Affinity Capture-RNA Homo sapiens
84 GPANK1  
Two-hybrid Homo sapiens
85 VGLL3  
Two-hybrid Homo sapiens
86 NEFL 4747
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
87 RPA3 6119
Proximity Label-MS Homo sapiens
88 RAB9A 9367
Proximity Label-MS Homo sapiens
89 TOP1 7150
Negative Genetic Homo sapiens
90 C1orf94  
Two-hybrid Homo sapiens
91 RPAP3 79657
Affinity Capture-MS Homo sapiens
92 ZNF587  
Two-hybrid Homo sapiens
93 PPP1R32  
Two-hybrid Homo sapiens
94 SHC3  
Two-hybrid Homo sapiens
95 MSN 4478
Two-hybrid Homo sapiens
96 CDKN1B 1027
Affinity Capture-Western Homo sapiens
97 Tsc2  
Affinity Capture-MS Mus musculus
98 LMNA 4000
Cross-Linking-MS (XL-MS) Homo sapiens
99 ZNF417  
Two-hybrid Homo sapiens
100 RRAGC 64121
Affinity Capture-Western Homo sapiens
101 ZNF765  
Two-hybrid Homo sapiens
102 FASN 2194
Negative Genetic Homo sapiens
103 TSHZ3  
Two-hybrid Homo sapiens
104 VPS37C 55048
Two-hybrid Homo sapiens
105 TBC1D7  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
106 PPM1H  
Proximity Label-MS Homo sapiens
107 WDR92 116143
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 EZR 7430
Affinity Capture-Western Homo sapiens
109 EDEM1  
Affinity Capture-MS Homo sapiens
110 NINL  
Proximity Label-MS Homo sapiens
111 RUVBL2 10856
Affinity Capture-MS Homo sapiens
112 TSC1 7248
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
113 DDX58 23586
Affinity Capture-RNA Homo sapiens
114 CDK1 983
Reconstituted Complex Homo sapiens
115 ACTA1 58
Co-localization Homo sapiens
116 KDM1A 23028
Two-hybrid Homo sapiens
117 RUVBL1 8607
Affinity Capture-MS Homo sapiens
118 MOV10 4343
Affinity Capture-RNA Homo sapiens
119 BECN1 8678
Affinity Capture-MS Homo sapiens
120 KRT38 8687
Affinity Capture-MS Homo sapiens
121 RAB7A 7879
Proximity Label-MS Homo sapiens
122 RAB11A 8766
Proximity Label-MS Homo sapiens
123 PIH1D1  
Affinity Capture-MS Homo sapiens
124 SAMD7  
Two-hybrid Homo sapiens
125 RAB5C 5878
Proximity Label-MS Homo sapiens
126 AQP1 358
Two-hybrid Homo sapiens
127 IKBKB 3551
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
128 LURAP1  
Affinity Capture-MS Homo sapiens
129 KDM5C  
Negative Genetic Homo sapiens
130 DERL1 79139
Proximity Label-MS Homo sapiens
View the network image/svg+xml



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