Gene ontology annotations for NPTN
Experiment description of studies that identified NPTN in sEVs
1
Experiment ID
489
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 6
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
2
Experiment ID
490
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 7
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
3
Experiment ID
491
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
4
Experiment ID
492
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 9
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
5
Experiment ID
426
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
6
Experiment ID
427
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
7
Experiment ID
494
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
Osteoarthritic cartilage
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
8
Experiment ID
496
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
Healthy cartilage
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
9
Experiment ID
497
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
T/C-28a2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
10
Experiment ID
498
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
T/C-28a2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
11
Experiment ID
207
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DKO-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
12
Experiment ID
208
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
Dks-8
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
13
Experiment ID
209
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DLD-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
14
Experiment ID
1203
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
34887515
Organism
Homo sapiens
Experiment description
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Colorectal cancer cells
Sample name
DiFi
Isolation/purification methods
Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein miRNA
Methods used in the study
Western blotting Mass spectrometry RNA sequencing
15
Experiment ID
419
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
16
Experiment ID
419
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
17
Experiment ID
420
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
18
Experiment ID
420
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
19
Experiment ID
363
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
20
Experiment ID
364
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
21
Experiment ID
365
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
22
Experiment ID
258
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✔
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
SKMEL28
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
23
Experiment ID
259
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✔
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
A375M
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
24
Experiment ID
260
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✔
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
1205Lu
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
25
Experiment ID
488
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Homo sapiens
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Mesenchymal stem cells
Sample name
UCMSC
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
26
Experiment ID
224
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25944692
Organism
Homo sapiens
Experiment description
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors
"Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name
Oncotarget
Publication year
2015
Sample
Neuroblastoma cells
Sample name
SH-SY5Y
Isolation/purification methods
Differential centrifugation Ultracentrifugation OptiPrep density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
27
Experiment ID
211
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
IGROV1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
28
Experiment ID
212
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
OVCAR-3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
29
Experiment ID
434
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
30
Experiment ID
435
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
31
Experiment ID
275
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25844599
Organism
Homo sapiens
Experiment description
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors
"Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name
Oncotarget
Publication year
2015
Sample
Prostate cancer cells
Sample name
DU145 - Docetaxel sensitive
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.12-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry/Flow cytometry/Western blotting
32
Experiment ID
274
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25844599
Organism
Homo sapiens
Experiment description
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors
"Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name
Oncotarget
Publication year
2015
Sample
Prostate cancer cells
Sample name
DU145 - Docetaxel resistant
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.18 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Flow cytometry Western blotting
33
Experiment ID
834
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
34
Experiment ID
835
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
35
Experiment ID
191
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20124223
Organism
Homo sapiens
Experiment description
Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors
"Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name
Mol Cell Proteomics
Publication year
2010
Sample
Squamous carcinoma cells
Sample name
Squamous carcinoma cell (A431)
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for NPTN
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
BRINP2
Affinity Capture-MS
Homo sapiens
2
ATR
Affinity Capture-MS
Homo sapiens
3
GCFC2
Affinity Capture-MS
Homo sapiens
4
NUP85
79902
Affinity Capture-MS
Homo sapiens
5
AGPAT5
55326
Affinity Capture-MS
Homo sapiens
6
COG7
91949
Affinity Capture-MS
Homo sapiens
7
SEC14L1
6397
Affinity Capture-MS
Homo sapiens
8
ORC4
Affinity Capture-MS
Homo sapiens
9
RRN3
Affinity Capture-MS
Homo sapiens
10
DOCK4
9732
Affinity Capture-MS
Homo sapiens
11
DHRS7
51635
Affinity Capture-MS
Homo sapiens
12
TFB2M
Affinity Capture-MS
Homo sapiens
13
SRPRB
58477
Affinity Capture-MS
Homo sapiens
14
CCDC132
55610
Affinity Capture-MS
Homo sapiens
15
BZW2
28969
Affinity Capture-MS
Homo sapiens
16
RPA2
6118
Proximity Label-MS
Homo sapiens
17
CAND2
23066
Affinity Capture-MS
Homo sapiens
18
MFN1
55669
Affinity Capture-MS
Homo sapiens
19
FOCAD
54914
Affinity Capture-MS
Homo sapiens
20
TUBB8
347688
Affinity Capture-MS
Homo sapiens
21
FUK
197258
Affinity Capture-MS
Homo sapiens
22
LTN1
26046
Affinity Capture-MS
Homo sapiens
23
SPNS1
83985
Co-fractionation
Homo sapiens
24
KIAA0368
23392
Affinity Capture-MS
Homo sapiens
25
UBC
7316
Reconstituted Complex
Homo sapiens
26
CSE1L
1434
Affinity Capture-MS
Homo sapiens
27
COG2
22796
Affinity Capture-MS
Homo sapiens
28
SCAI
Affinity Capture-MS
Homo sapiens
29
INTS1
26173
Affinity Capture-MS
Homo sapiens
30
FHOD1
29109
Affinity Capture-MS
Homo sapiens
31
STAG1
Affinity Capture-MS
Homo sapiens
32
COQ5
Affinity Capture-MS
Homo sapiens
33
GCN1L1
10985
Affinity Capture-MS
Homo sapiens
34
C17orf75
64149
Affinity Capture-MS
Homo sapiens
35
GFM2
84340
Affinity Capture-MS
Homo sapiens
36
PI4KA
5297
Affinity Capture-MS
Homo sapiens
37
ANKRD44
91526
Affinity Capture-MS
Homo sapiens
38
SURF2
Two-hybrid
Homo sapiens
39
XPO7
23039
Affinity Capture-MS
Homo sapiens
40
XPO4
64328
Affinity Capture-MS
Homo sapiens
41
PLEKHG4
Affinity Capture-MS
Homo sapiens
42
MMS22L
Affinity Capture-MS
Homo sapiens
43
STK26
51765
Affinity Capture-MS
Homo sapiens
44
MON2
23041
Affinity Capture-MS
Homo sapiens
45
RINT1
60561
Affinity Capture-MS
Homo sapiens
46
MDN1
23195
Affinity Capture-MS
Homo sapiens
47
D2HGDH
728294
Affinity Capture-MS
Homo sapiens
48
BRAT1
221927
Affinity Capture-MS
Homo sapiens
49
FAM174A
345757
Affinity Capture-MS
Homo sapiens
50
TELO2
9894
Affinity Capture-MS
Homo sapiens
51
NBEAL2
23218
Affinity Capture-MS
Homo sapiens
52
MLST8
64223
Affinity Capture-MS
Homo sapiens
53
COG4
25839
Affinity Capture-MS
Homo sapiens
54
AGTR1
Two-hybrid
Homo sapiens
55
IPO8
10526
Affinity Capture-MS
Homo sapiens
56
RANBP17
Affinity Capture-MS
Homo sapiens
57
SLC25A19
Affinity Capture-MS
Homo sapiens
58
SFXN2
Affinity Capture-MS
Homo sapiens
59
ARMC6
93436
Affinity Capture-MS
Homo sapiens
60
FIG4
9896
Affinity Capture-MS
Homo sapiens
61
UTP20
27340
Affinity Capture-MS
Homo sapiens
62
XRCC3
Affinity Capture-MS
Homo sapiens
63
BTAF1
9044
Affinity Capture-MS
Homo sapiens
64
LGALS9
3965
Affinity Capture-MS
Homo sapiens
65
SNX27
81609
Affinity Capture-MS
Homo sapiens
66
ARFGEF2
10564
Affinity Capture-MS
Homo sapiens
67
EXOC7
23265
Affinity Capture-MS
Homo sapiens
68
ADCK4
79934
Affinity Capture-MS
Homo sapiens
69
GNPAT
8443
Affinity Capture-MS
Homo sapiens
70
DYNC2LI1
51626
Affinity Capture-MS
Homo sapiens
71
TMEM87A
25963
Affinity Capture-MS
Homo sapiens
72
SLC25A29
Affinity Capture-MS
Homo sapiens
73
KIF14
9928
Affinity Capture-MS
Homo sapiens
74
FASTKD1
Affinity Capture-MS
Homo sapiens
75
TSC2
7249
Affinity Capture-MS
Homo sapiens
76
UCKL1
54963
Affinity Capture-MS
Homo sapiens
77
DDX19B
11269
Affinity Capture-MS
Homo sapiens
78
HEATR1
55127
Affinity Capture-MS
Homo sapiens
79
BSG
682
Co-fractionation
Homo sapiens
80
PDK1
5163
Affinity Capture-MS
Homo sapiens
81
ACTA2
59
Affinity Capture-MS
Homo sapiens
82
GEMIN5
25929
Affinity Capture-MS
Homo sapiens
83
SERPINB4
6318
Affinity Capture-MS
Homo sapiens
84
IPO11
51194
Affinity Capture-MS
Homo sapiens
85
SYMPK
8189
Affinity Capture-MS
Homo sapiens
86
P4HA1
5033
Co-fractionation
Homo sapiens
87
FASTKD5
Affinity Capture-MS
Homo sapiens
88
SMG8
55181
Affinity Capture-MS
Homo sapiens
89
TNPO1
3842
Affinity Capture-MS
Homo sapiens
90
VPS51
738
Affinity Capture-MS
Homo sapiens
91
SFXN3
81855
Affinity Capture-MS
Homo sapiens
92
SIMC1
Affinity Capture-MS
Homo sapiens
93
TUBA1A
7846
Affinity Capture-MS
Homo sapiens
94
MCAM
4162
Proximity Label-MS
Homo sapiens
95
CLTCL1
8218
Affinity Capture-MS
Homo sapiens
96
HTT
3064
Affinity Capture-MS
Homo sapiens
97
RNF213
57674
Affinity Capture-MS
Homo sapiens
98
SMIM11
Cross-Linking-MS (XL-MS)
Homo sapiens
99
DDX20
11218
Affinity Capture-MS
Homo sapiens
100
DOPEY1
Affinity Capture-MS
Homo sapiens
101
FAM118B
Affinity Capture-MS
Homo sapiens
102
TUBA1C
84790
Affinity Capture-MS
Homo sapiens
103
FANCG
2189
Affinity Capture-MS
Homo sapiens
104
PDS5A
23244
Affinity Capture-MS
Homo sapiens
105
PDS5B
23047
Affinity Capture-MS
Homo sapiens
106
NOC4L
79050
Affinity Capture-MS
Homo sapiens
107
PIKFYVE
200576
Affinity Capture-MS
Homo sapiens
108
EXOC6
54536
Affinity Capture-MS
Homo sapiens
109
MMS19
64210
Affinity Capture-MS
Homo sapiens
110
COG3
83548
Affinity Capture-MS
Homo sapiens
111
HEMK1
Affinity Capture-MS
Homo sapiens
112
SGSM3
Affinity Capture-MS
Homo sapiens
113
VMA21
Co-fractionation
Homo sapiens
114
TNPO2
30000
Affinity Capture-MS
Homo sapiens
115
FAM127A
Affinity Capture-MS
Homo sapiens
116
COG5
10466
Affinity Capture-MS
Homo sapiens
117
TSC1
7248
Affinity Capture-MS
Homo sapiens
118
TNFAIP8L2
Affinity Capture-MS
Homo sapiens
119
STAG2
10735
Affinity Capture-MS
Homo sapiens
120
PDSS2
Affinity Capture-MS
Homo sapiens
121
TNFAIP2
7127
Affinity Capture-MS
Homo sapiens
122
SLC25A5
292
Co-fractionation
Homo sapiens
123
ATP6V1H
51606
Affinity Capture-MS
Homo sapiens
124
HEATR6
Affinity Capture-MS
Homo sapiens
125
TTI1
9675
Affinity Capture-MS
Homo sapiens
126
TONSL
Affinity Capture-MS
Homo sapiens
127
APOE
348
Affinity Capture-MS
Homo sapiens
128
PICK1
Affinity Capture-MS
Homo sapiens
129
CYB5B
80777
Co-fractionation
Homo sapiens
130
LARS2
23395
Affinity Capture-MS
Homo sapiens
131
EARS2
Affinity Capture-MS
Homo sapiens
132
DMXL2
Affinity Capture-MS
Homo sapiens
133
FANCD2
Affinity Capture-MS
Homo sapiens
134
CANX
821
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
135
TARBP1
6894
Affinity Capture-MS
Homo sapiens
136
APOL2
23780
Affinity Capture-MS
Homo sapiens
137
KIAA1244
Affinity Capture-MS
Homo sapiens
138
XPO1
7514
Affinity Capture-MS
Homo sapiens
139
XPO5
57510
Affinity Capture-MS
Homo sapiens
140
ATP2B1
490
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
141
SARM1
23098
Affinity Capture-MS
Homo sapiens
142
ARFGEF1
10565
Affinity Capture-MS
Homo sapiens
143
C17orf78
Affinity Capture-MS
Homo sapiens
144
ACTBL2
345651
Affinity Capture-MS
Homo sapiens
145
ACAD10
Affinity Capture-MS
Homo sapiens
146
TNPO3
23534
Affinity Capture-MS
Homo sapiens
147
FBXO6
26270
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
148
S100A7A
338324
Affinity Capture-MS
Homo sapiens
149
KIAA1524
57650
Affinity Capture-MS
Homo sapiens
150
TBCD
6904
Affinity Capture-MS
Homo sapiens
151
TSPYL1
7259
Affinity Capture-MS
Homo sapiens
152
MSTO1
55154
Affinity Capture-MS
Homo sapiens
153
COG1
9382
Affinity Capture-MS
Homo sapiens
154
SETX
Affinity Capture-MS
Homo sapiens
155
TUBA4A
7277
Affinity Capture-MS
Homo sapiens
156
GEMIN4
50628
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
157
COG6
57511
Affinity Capture-MS
Homo sapiens
158
GANAB
23193
Affinity Capture-MS
Homo sapiens
159
GBF1
8729
Affinity Capture-MS
Homo sapiens
160
RPA3
6119
Proximity Label-MS
Homo sapiens
161
ATP2A1
487
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
162
RELT
Affinity Capture-MS
Homo sapiens
163
COG8
84342
Affinity Capture-MS
Homo sapiens
164
RAB38
23682
Affinity Capture-MS
Homo sapiens
165
MTOR
2475
Affinity Capture-MS
Homo sapiens
166
C14orf80
Affinity Capture-MS
Homo sapiens
167
USP24
23358
Affinity Capture-MS
Homo sapiens
168
MROH1
727957
Affinity Capture-MS
Homo sapiens
169
FBXL12
54850
Affinity Capture-MS
Homo sapiens
170
ATM
472
Affinity Capture-MS
Homo sapiens
171
OPA1
4976
Affinity Capture-MS
Homo sapiens
172
RHOB
388
Proximity Label-MS
Homo sapiens
173
SUGCT
Affinity Capture-MS
Homo sapiens
174
LGALS8
3964
Affinity Capture-MS
Homo sapiens
175
BDH1
622
Affinity Capture-MS
Homo sapiens
176
THADA
63892
Affinity Capture-MS
Homo sapiens
177
NDUFV2
4729
Co-fractionation
Homo sapiens
178
INTS5
80789
Affinity Capture-MS
Homo sapiens
179
UBXN1
51035
Co-fractionation
Homo sapiens
180
SNX5
27131
Affinity Capture-MS
Homo sapiens
181
CPT1A
1374
Affinity Capture-MS
Homo sapiens
182
EXOC5
10640
Affinity Capture-MS
Homo sapiens
183
ATP2B3
492
Affinity Capture-MS
Homo sapiens
184
NIT2
56954
Co-fractionation
Homo sapiens
185
AK4
205
Affinity Capture-MS
Homo sapiens
186
SLC25A33
Affinity Capture-MS
Homo sapiens
187
HEATR5B
54497
Affinity Capture-MS
Homo sapiens
188
ABCB6
10058
Co-fractionation
Homo sapiens
189
RAD17
Affinity Capture-MS
Homo sapiens
190
SLC25A6
293
Affinity Capture-MS
Homo sapiens
191
ATP2B4
493
Affinity Capture-MS
Homo sapiens
192
KNTC1
9735
Affinity Capture-MS
Homo sapiens
193
XPO6
23214
Affinity Capture-MS
Homo sapiens
194
TTC28
23331
Affinity Capture-MS
Homo sapiens
195
ZFYVE26
23503
Affinity Capture-MS
Homo sapiens
196
VAC14
55697
Affinity Capture-MS
Homo sapiens
197
DSTYK
25778
Affinity Capture-MS
Homo sapiens
198
WDR35
57539
Affinity Capture-MS
Homo sapiens
199
RPS6KA4
8986
Affinity Capture-MS
Homo sapiens
200
LGALS3
3958
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
201
RRP1
8568
Affinity Capture-MS
Homo sapiens
202
DOPEY2
9980
Affinity Capture-MS
Homo sapiens
203
DNAAF5
54919
Affinity Capture-MS
Homo sapiens
204
TUBB3
10381
Affinity Capture-MS
Homo sapiens
205
INTS8
Affinity Capture-MS
Homo sapiens
206
Htt
Affinity Capture-MS
Mus musculus
207
TTK
7272
Affinity Capture-MS
Homo sapiens
208
EXOC2
55770
Affinity Capture-MS
Homo sapiens
209
ATP2A2
488
Co-fractionation
Homo sapiens
210
SERPINB3
6317
Affinity Capture-MS
Homo sapiens
211
CERS5
91012
Affinity Capture-MS
Homo sapiens
212
ZW10
9183
Affinity Capture-MS
Homo sapiens
213
ANAPC2
29882
Affinity Capture-MS
Homo sapiens
214
NBEAL1
65065
Affinity Capture-MS
Homo sapiens
215
PRAME
Affinity Capture-MS
Homo sapiens
216
SPG11
80208
Affinity Capture-MS
Homo sapiens
217
RUFY2
Affinity Capture-MS
Homo sapiens
218
TTI2
80185
Affinity Capture-MS
Homo sapiens
219
EDC4
23644
Affinity Capture-MS
Homo sapiens
220
BCL2L13
23786
Co-fractionation
Homo sapiens
221
TBRG4
9238
Affinity Capture-MS
Homo sapiens
222
SLC25A4
291
Affinity Capture-MS
Homo sapiens
223
MIEF2
Affinity Capture-MS
Homo sapiens
224
IPO4
79711
Affinity Capture-MS
Homo sapiens
225
RUSC1
Affinity Capture-MS
Homo sapiens
226
ATP2B2
491
Affinity Capture-MS
Homo sapiens
227
SMG1
23049
Affinity Capture-MS
Homo sapiens
228
PYCR1
5831
Affinity Capture-MS
Homo sapiens
229
IPO13
9670
Affinity Capture-MS
Homo sapiens
230
ABHD6
57406
Affinity Capture-MS
Homo sapiens
231
TXNDC11
51061
Affinity Capture-MS
Homo sapiens
232
LCN2
3934
Affinity Capture-MS
Homo sapiens
233
DPH6
Affinity Capture-MS
Homo sapiens
234
HSPA12A
259217
Affinity Capture-MS
Homo sapiens
235
ZWILCH
55055
Affinity Capture-MS
Homo sapiens
236
AP5Z1
9907
Affinity Capture-MS
Homo sapiens
237
DYNC2H1
79659
Affinity Capture-MS
Homo sapiens
238
FAM210A
Affinity Capture-MS
Homo sapiens
239
FANCA
Affinity Capture-MS
Homo sapiens
View the network
image/svg+xml
Pathways in which NPTN is involved