Gene description for DNAAF5
Gene name dynein, axonemal, assembly factor 5
Gene symbol DNAAF5
Other names/aliases CILD18
HEATR2
Species Homo sapiens
 Database cross references - DNAAF5
ExoCarta ExoCarta_54919
Entrez Gene 54919
HGNC 26013
MIM 614864
UniProt Q86Y56  
 DNAAF5 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for DNAAF5
Molecular Function
    dynein intermediate chain binding GO:0045505 IPI
Biological Process
    inner dynein arm assembly GO:0036159 IMP
    cilium movement GO:0003341 IMP
    outer dynein arm assembly GO:0036158 IMP
Subcellular Localization
    motile cilium GO:0031514 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified DNAAF5 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DNAAF5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CASP4  
Affinity Capture-MS Homo sapiens
2 BRF2  
Affinity Capture-MS Homo sapiens
3 MAGED1  
Affinity Capture-MS Homo sapiens
4 MAGEA6  
Affinity Capture-MS Homo sapiens
5 FEZ1  
Two-hybrid Homo sapiens
6 PHLDA3  
Affinity Capture-MS Homo sapiens
7 PTP4A3  
Affinity Capture-MS Homo sapiens
8 CIAO1 9391
Affinity Capture-MS Homo sapiens
9 ZBTB16  
Two-hybrid Homo sapiens
10 PNKP 11284
Affinity Capture-MS Homo sapiens
11 ILK 3611
Affinity Capture-MS Homo sapiens
12 PGRMC1 10857
Affinity Capture-MS Homo sapiens
13 GSTK1 373156
Affinity Capture-MS Homo sapiens
14 TSC22D1  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DNAAF5 is involved
No pathways found





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