Gene description for B3GAT3
Gene name beta-1,3-glucuronyltransferase 3
Gene symbol B3GAT3
Other names/aliases GLCATI
glcUAT-I
Species Homo sapiens
 Database cross references - B3GAT3
ExoCarta ExoCarta_26229
Vesiclepedia VP_26229
Entrez Gene 26229
HGNC 923
MIM 606374
UniProt O94766  
 B3GAT3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Saliva 19199708    
 Gene ontology annotations for B3GAT3
Molecular Function
    protein binding GO:0005515 IPI
    galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity GO:0015018 IBA
    galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity GO:0015018 NAS
    glucuronosyltransferase activity GO:0015020 IDA
    glucuronosyltransferase activity GO:0015020 IMP
    metal ion binding GO:0046872 IEA
    protein phosphatase activator activity GO:0072542 IDA
Biological Process
    carbohydrate metabolic process GO:0005975 IBA
    glycosaminoglycan biosynthetic process GO:0006024 IDA
    glycosaminoglycan biosynthetic process GO:0006024 IMP
    glycosaminoglycan biosynthetic process GO:0006024 NAS
    protein glycosylation GO:0006486 IEA
    heparan sulfate proteoglycan biosynthetic process GO:0015012 IDA
    heparan sulfate proteoglycan biosynthetic process GO:0015012 IMP
    positive regulation of catalytic activity GO:0043085 IDA
    chondroitin sulfate proteoglycan biosynthetic process GO:0050650 IBA
    chondroitin sulfate proteoglycan biosynthetic process GO:0050650 IDA
    chondroitin sulfate proteoglycan biosynthetic process GO:0050650 IMP
    dermatan sulfate proteoglycan biosynthetic process GO:0050651 IDA
    dermatan sulfate proteoglycan biosynthetic process GO:0050651 IMP
    positive regulation of intracellular protein transport GO:0090316 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IBA
    Golgi membrane GO:0000139 TAS
    Golgi apparatus GO:0005794 IDA
    cis-Golgi network GO:0005801 IDA
    membrane GO:0016020 HDA
    membrane GO:0016020 NAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified B3GAT3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for B3GAT3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC22B 9554
Two-hybrid Homo sapiens
2 HMOX1 3162
Affinity Capture-MS Homo sapiens
3 RAMP3  
Affinity Capture-MS Homo sapiens
4 CSGALNACT2 55454
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 TSPAN15 23555
Affinity Capture-MS Homo sapiens
6 ST8SIA3  
Affinity Capture-MS Homo sapiens
7 SYNGR1 9145
Affinity Capture-MS Homo sapiens
8 SLC39A4 55630
Affinity Capture-MS Homo sapiens
9 NOP9 161424
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 SGCA  
Affinity Capture-MS Homo sapiens
11 KIAA2013 90231
Affinity Capture-MS Homo sapiens
12 CCR4 1233
Two-hybrid Homo sapiens
13 JPH1 56704
Affinity Capture-MS Homo sapiens
14 TMEM95  
Affinity Capture-MS Homo sapiens
15 PLD4  
Affinity Capture-MS Homo sapiens
16 SETX  
Affinity Capture-MS Homo sapiens
17 SLC22A9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 PDE2A  
Affinity Capture-MS Homo sapiens
19 HEATR3 55027
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 CCR2  
Two-hybrid Homo sapiens
21 FAM174A 345757
Affinity Capture-MS Homo sapiens
22 HYAL2 8692
Affinity Capture-MS Homo sapiens
23 NDST3  
Affinity Capture-MS Homo sapiens
24 MGAT1 4245
Affinity Capture-MS Homo sapiens
25 SLC31A1 1317
Affinity Capture-MS Homo sapiens
26 NLGN2 57555
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 NMU  
Affinity Capture-MS Homo sapiens
28 PIGG  
Affinity Capture-MS Homo sapiens
29 GPX8 493869
Affinity Capture-MS Homo sapiens
30 TMEM106A  
Affinity Capture-MS Homo sapiens
31 ATP13A3 79572
Affinity Capture-MS Homo sapiens
32 TMEM59 9528
Affinity Capture-MS Homo sapiens
33 CHST12 55501
Affinity Capture-MS Homo sapiens
34 GOLIM4 27333
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 EXTL3 2137
Affinity Capture-MS Homo sapiens
36 CD70 970
Affinity Capture-MS Homo sapiens
37 P2RY8  
Affinity Capture-MS Homo sapiens
38 GPR35  
Two-hybrid Homo sapiens
39 PBXIP1 57326
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 GPR182  
Affinity Capture-MS Homo sapiens
41 TMEM259 91304
Affinity Capture-MS Homo sapiens
42 NLGN1  
Affinity Capture-MS Homo sapiens
43 XYLT2 64132
Affinity Capture-MS Homo sapiens
44 TMEM171  
Affinity Capture-MS Homo sapiens
45 HAUS7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 HNRNPD 3184
Two-hybrid Homo sapiens
47 TMEM106B 54664
Affinity Capture-MS Homo sapiens
48 CIAO1 9391
Affinity Capture-MS Homo sapiens
49 GPR108 56927
Affinity Capture-MS Homo sapiens
50 ZDHHC12  
Affinity Capture-MS Homo sapiens
51 TMEM160 54958
Affinity Capture-MS Homo sapiens
52 GAA 2548
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 KLRG2 346689
Affinity Capture-MS Homo sapiens
54 SYP  
Affinity Capture-MS Homo sapiens
55 ARMC6 93436
Affinity Capture-MS Homo sapiens
56 XPO4 64328
Affinity Capture-MS Homo sapiens
57 COLEC12 81035
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 SLC30A7 148867
Affinity Capture-MS Homo sapiens
59 B3GAT3 26229
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
60 DYM  
Affinity Capture-MS Homo sapiens
61 RNF185  
Affinity Capture-MS Homo sapiens
62 LEMD3  
Affinity Capture-MS Homo sapiens
63 KCNK16  
Affinity Capture-MS Homo sapiens
64 GFRA3 2676
Affinity Capture-MS Homo sapiens
65 NCAPG 64151
Affinity Capture-MS Homo sapiens
66 NRG1 3084
Affinity Capture-MS Homo sapiens
67 STX6 10228
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 HMGCR  
Affinity Capture-MS Homo sapiens
69 ADORA2B  
Affinity Capture-MS Homo sapiens
70 TACR1  
Affinity Capture-MS Homo sapiens
71 ABCB8 11194
Affinity Capture-MS Homo sapiens
72 LDLRAD1  
Affinity Capture-MS Homo sapiens
73 CLEC2D  
Affinity Capture-MS Homo sapiens
74 KCNC4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 DNAAF5 54919
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 PIGK 10026
Affinity Capture-MS Homo sapiens
77 SAAL1 113174
Affinity Capture-MS Homo sapiens
78 PTPRO 5800
Affinity Capture-MS Homo sapiens
79 SFTPC  
Affinity Capture-MS Homo sapiens
80 PLD3 23646
Affinity Capture-MS Homo sapiens
81 SEC62 7095
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 TMEM79  
Affinity Capture-MS Homo sapiens
83 KIAA1468 57614
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 WDR13 64743
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 ATP5C1 509
Affinity Capture-MS Homo sapiens
86 ADGRE5 976
Affinity Capture-MS Homo sapiens
87 CKAP4 10970
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 ZDHHC13 54503
Affinity Capture-MS Homo sapiens
89 S1PR4  
Affinity Capture-MS Homo sapiens
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