Gene description for B3GAT3
Gene name beta-1,3-glucuronyltransferase 3
Gene symbol B3GAT3
Other names/aliases GLCATI
glcUAT-I
Species Homo sapiens
 Database cross references - B3GAT3
ExoCarta ExoCarta_26229
Entrez Gene 26229
HGNC 923
MIM 606374
UniProt O94766  
 B3GAT3 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Saliva 19199708    
 Gene ontology annotations for B3GAT3
Molecular Function
    metal ion binding GO:0046872 IEA
    protein binding GO:0005515 IPI
    galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity GO:0015018 NAS
    glucuronosyltransferase activity GO:0015020 IMP
    protein phosphatase activator activity GO:0072542 IDA
Biological Process
    protein glycosylation GO:0006486 IEA
    small molecule metabolic process GO:0044281 TAS
    heparan sulfate proteoglycan biosynthetic process GO:0015012 IDA
    chondroitin sulfate metabolic process GO:0030204 TAS
    positive regulation of intracellular protein transport GO:0090316 IMP
    carbohydrate metabolic process GO:0005975 TAS
    chondroitin sulfate proteoglycan biosynthetic process GO:0050650 IDA
    positive regulation of catalytic activity GO:0043085 IDA
    glycosaminoglycan metabolic process GO:0030203 TAS
    dermatan sulfate proteoglycan biosynthetic process GO:0050651 IDA
    glycosaminoglycan biosynthetic process GO:0006024 NAS
Subcellular Localization
    cis-Golgi network GO:0005801 IDA
    integral component of membrane GO:0016021 NAS
    Golgi apparatus GO:0005794 IDA
    Golgi membrane GO:0000139 TAS
    membrane GO:0016020 IDA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified B3GAT3 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 66
ISEV standards
IEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD63|CD81
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for B3GAT3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 B3GAT3 26229
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which B3GAT3 is involved
PathwayEvidenceSource
A tetrasaccharide linker sequence is required for GAG synthesis TAS Reactome
Defective B3GAT3 causes JDSSDHD TAS Reactome





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