Gene description for MGAT1
Gene name mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
Gene symbol MGAT1
Other names/aliases GLCNAC-TI
GLCT1
GLYT1
GNT-1
GNT-I
MGAT
Species Homo sapiens
 Database cross references - MGAT1
ExoCarta ExoCarta_4245
Vesiclepedia VP_4245
Entrez Gene 4245
HGNC 7044
MIM 160995
UniProt P26572  
 MGAT1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Saliva 19199708    
 Gene ontology annotations for MGAT1
Molecular Function
    alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity GO:0003827 IBA
    alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity GO:0003827 IDA
    alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity GO:0003827 TAS
    protein binding GO:0005515 IPI
    acetylglucosaminyltransferase activity GO:0008375 TAS
    manganese ion binding GO:0030145 IDA
Biological Process
    in utero embryonic development GO:0001701 IEA
    UDP-N-acetylglucosamine catabolic process GO:0006049 IEA
    protein N-linked glycosylation GO:0006487 IBA
    protein N-linked glycosylation via asparagine GO:0018279 IDA
    viral protein processing GO:0019082 TAS
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    Golgi apparatus GO:0005794 IBA
    membrane GO:0016020 HDA
    endoplasmic reticulum-Golgi intermediate compartment membrane GO:0033116 TAS
    perinuclear region of cytoplasm GO:0048471 IEA
    extracellular exosome GO:0070062 HDA
    extracellular vesicle GO:1903561 HDA
 Experiment description of studies that identified MGAT1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for MGAT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NRG1 3084
Affinity Capture-MS Homo sapiens
2 CCL3  
Affinity Capture-MS Homo sapiens
3 MCOLN2  
Affinity Capture-MS Homo sapiens
4 Anapc13  
Affinity Capture-MS Mus musculus
5 TMEM59 9528
Affinity Capture-MS Homo sapiens
6 SLC39A4 55630
Affinity Capture-MS Homo sapiens
7 TMPRSS11B 132724
Affinity Capture-MS Homo sapiens
8 HPN  
Affinity Capture-MS Homo sapiens
9 MME 4311
Affinity Capture-MS Homo sapiens
10 HTR3C  
Affinity Capture-MS Homo sapiens
11 PDGFRA 5156
Affinity Capture-MS Homo sapiens
12 FUT8 2530
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 ADPGK 83440
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 DDX58 23586
Affinity Capture-RNA Homo sapiens
15 TNFSF18 8995
Affinity Capture-MS Homo sapiens
16 Mus81  
Affinity Capture-MS Mus musculus
17 CLEC12B  
Affinity Capture-MS Homo sapiens
18 KDSR 2531
Affinity Capture-MS Homo sapiens
19 MAPK6  
Two-hybrid Homo sapiens
20 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
21 SMAD9  
Two-hybrid Homo sapiens
22 RELL1 768211
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 TCTN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 GINM1 116254
Affinity Capture-MS Homo sapiens
25 B3GAT3 26229
Affinity Capture-MS Homo sapiens
26 ST14 6768
Affinity Capture-MS Homo sapiens
27 Mad2l1 56150
Affinity Capture-MS Mus musculus
28 PTCH1  
Affinity Capture-MS Homo sapiens
29 TMPRSS13  
Affinity Capture-MS Homo sapiens
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