Gene description for FUT8
Gene name fucosyltransferase 8 (alpha (1,6) fucosyltransferase)
Gene symbol FUT8
Other names/aliases -
Species Homo sapiens
 Database cross references - FUT8
ExoCarta ExoCarta_2530
Vesiclepedia VP_2530
Entrez Gene 2530
HGNC 4019
MIM 602589
UniProt Q9BYC5  
 FUT8 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Saliva 19199708    
 Gene ontology annotations for FUT8
Molecular Function
    protein binding GO:0005515 IPI
    glycoprotein 6-alpha-L-fucosyltransferase activity GO:0008424 IDA
    glycoprotein 6-alpha-L-fucosyltransferase activity GO:0008424 TAS
    SH3 domain binding GO:0017124 IEA
    alpha-(1->6)-fucosyltransferase activity GO:0046921 IBA
Biological Process
    in utero embryonic development GO:0001701 NAS
    protein N-linked glycosylation GO:0006487 IBA
    N-glycan processing GO:0006491 TAS
    transforming growth factor beta receptor signaling pathway GO:0007179 IEA
    integrin-mediated signaling pathway GO:0007229 IEA
    respiratory gaseous exchange by respiratory system GO:0007585 IEA
    oligosaccharide biosynthetic process GO:0009312 TAS
    regulation of gene expression GO:0010468 IEA
    fibroblast migration GO:0010761 IEA
    protein N-linked glycosylation via asparagine GO:0018279 IDA
    viral protein processing GO:0019082 TAS
    N-glycan fucosylation GO:0036071 IBA
    L-fucose catabolic process GO:0042355 NAS
    receptor metabolic process GO:0043112 IEA
    GDP-L-fucose metabolic process GO:0046368 IDA
    regulation of cellular response to oxidative stress GO:1900407 IEA
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    Golgi apparatus GO:0005794 NAS
    membrane GO:0016020 HDA
    Golgi cisterna membrane GO:0032580 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified FUT8 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for FUT8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCL3  
Affinity Capture-MS Homo sapiens
2 RAMP3  
Affinity Capture-MS Homo sapiens
3 C5AR1  
Affinity Capture-MS Homo sapiens
4 CSGALNACT2 55454
Affinity Capture-MS Homo sapiens
5 CELSR1 9620
Affinity Capture-MS Homo sapiens
6 CHRNA4  
Affinity Capture-MS Homo sapiens
7 CACNA2D2 9254
Affinity Capture-MS Homo sapiens
8 SLC39A4 55630
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 NRSN1  
Affinity Capture-MS Homo sapiens
10 HPN  
Affinity Capture-MS Homo sapiens
11 BSCL2  
Affinity Capture-MS Homo sapiens
12 MFAP4 4239
Affinity Capture-MS Homo sapiens
13 SYVN1 84447
Affinity Capture-MS Homo sapiens
14 SLC30A4 7782
Affinity Capture-MS Homo sapiens
15 TNFSF18 8995
Affinity Capture-MS Homo sapiens
16 PDE2A  
Affinity Capture-MS Homo sapiens
17 SERINC2 347735
Affinity Capture-MS Homo sapiens
18 CTLA4  
Affinity Capture-MS Homo sapiens
19 HSPA5 3309
Affinity Capture-MS Homo sapiens
20 SEL1L 6400
Affinity Capture-MS Homo sapiens
21 STS 412
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 FAM174A 345757
Affinity Capture-MS Homo sapiens
23 STX8 9482
Affinity Capture-MS Homo sapiens
24 CRELD2 79174
Affinity Capture-MS Homo sapiens
25 MGAT1 4245
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 OLFM4 10562
Affinity Capture-MS Homo sapiens
27 STX18 53407
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 TMX3 54495
Affinity Capture-MS Homo sapiens
29 CD200R1 131450
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 TMEM106A  
Affinity Capture-MS Homo sapiens
31 RPL27 6155
Co-fractionation Homo sapiens
32 HTR3C  
Affinity Capture-MS Homo sapiens
33 NOMO1 23420
Affinity Capture-MS Homo sapiens
34 MAN2A1 4124
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 ITGAV 3685
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 CEACAM21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 CACNG7  
Affinity Capture-MS Homo sapiens
38 PDCD1LG2 80380
Affinity Capture-MS Homo sapiens
39 ACIN1 22985
Co-fractionation Homo sapiens
40 KCNK18  
Affinity Capture-MS Homo sapiens
41 RELL2  
Affinity Capture-MS Homo sapiens
42 KDSR 2531
Affinity Capture-MS Homo sapiens
43 CHRNB2  
Affinity Capture-MS Homo sapiens
44 PBXIP1 57326
Affinity Capture-MS Homo sapiens
45 MAN2A2 4122
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 GJB6  
Affinity Capture-MS Homo sapiens
47 SLC25A17 10478
Co-fractionation Homo sapiens
48 B3GALNT1 8706
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 TMPRSS13  
Affinity Capture-MS Homo sapiens
50 PCDHA8  
Affinity Capture-MS Homo sapiens
51 HTR3A  
Affinity Capture-MS Homo sapiens
52 MCOLN2  
Affinity Capture-MS Homo sapiens
53 ADAM15 8751
Affinity Capture-MS Homo sapiens
54 TTYH1  
Affinity Capture-MS Homo sapiens
55 ICAM2 3384
Affinity Capture-MS Homo sapiens
56 CRLF2 64109
Affinity Capture-MS Homo sapiens
57 SMIM5  
Affinity Capture-MS Homo sapiens
58 ADCY9 115
Affinity Capture-MS Homo sapiens
59 CHST8 64377
Affinity Capture-MS Homo sapiens
60 PDGFRA 5156
Affinity Capture-MS Homo sapiens
61 PRG2  
Affinity Capture-MS Homo sapiens
62 TMEM258  
Affinity Capture-MS Homo sapiens
63 NIPAL1  
Affinity Capture-MS Homo sapiens
64 TMEM74  
Affinity Capture-MS Homo sapiens
65 ECE1 1889
Affinity Capture-MS Homo sapiens
66 KCNE3  
Affinity Capture-MS Homo sapiens
67 CHRNA9  
Affinity Capture-MS Homo sapiens
68 TMEM132A 54972
Affinity Capture-MS Homo sapiens
69 RPA3 6119
Proximity Label-MS Homo sapiens
70 PLVAP 83483
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 DNAJC3 5611
Affinity Capture-MS Homo sapiens
72 ICAM3 3385
Affinity Capture-MS Homo sapiens
73 MPPE1 65258
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 GABRD  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 SLC9A6 10479
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 UBE2J1 51465
Affinity Capture-MS Homo sapiens
77 IFNGR2  
Affinity Capture-MS Homo sapiens
78 DHX15 1665
Co-fractionation Homo sapiens
79 CCR6  
Affinity Capture-MS Homo sapiens
80 NPTX1  
Affinity Capture-MS Homo sapiens
81 WDTC1 23038
Affinity Capture-MS Homo sapiens
82 SIDT2 51092
Affinity Capture-MS Homo sapiens
83 TMEM30B 161291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 CHST5  
Affinity Capture-MS Homo sapiens
85 TSPAN15 23555
Affinity Capture-MS Homo sapiens
86 OS9 10956
Affinity Capture-MS Homo sapiens
87 PLTP 5360
Affinity Capture-MS Homo sapiens
88 FGL2 10875
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 CLEC12B  
Affinity Capture-MS Homo sapiens
90 ERGIC2 51290
Affinity Capture-MS Homo sapiens
91 SEC62 7095
Affinity Capture-MS Homo sapiens
92 SPPL2B 56928
Affinity Capture-MS Homo sapiens
93 LPAR2 9170
Affinity Capture-MS Homo sapiens
94 ADGRE5 976
Affinity Capture-MS Homo sapiens
95 HTR1B  
Affinity Capture-MS Homo sapiens
96 KRTCAP3 200634
Affinity Capture-MS Homo sapiens
97 PTGFRN 5738
Affinity Capture-MS Homo sapiens
98 S1PR4  
Affinity Capture-MS Homo sapiens
99 DKKL1  
Affinity Capture-MS Homo sapiens
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