Gene description for DNAJC3
Gene name DnaJ (Hsp40) homolog, subfamily C, member 3
Gene symbol DNAJC3
Other names/aliases ACPHD
ERdj6
HP58
P58
P58IPK
PRKRI
Species Homo sapiens
 Database cross references - DNAJC3
ExoCarta ExoCarta_5611
Vesiclepedia VP_5611
Entrez Gene 5611
HGNC 9439
MIM 601184
UniProt Q13217  
 DNAJC3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Saliva 19199708    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for DNAJC3
Molecular Function
    protein kinase inhibitor activity GO:0004860 ISS
    protein kinase binding GO:0019901 ISS
    protein-folding chaperone binding GO:0051087 IBA
    misfolded protein binding GO:0051787 IBA
Biological Process
    response to unfolded protein GO:0006986 IEA
    protein folding in endoplasmic reticulum GO:0034975 IBA
    positive regulation of translation initiation in response to endoplasmic reticulum stress GO:0036494 ISS
    negative regulation of apoptotic process GO:0043066 IMP
    proteolysis involved in protein catabolic process GO:0051603 IEA
    defense response to virus GO:0051607 IEA
    cellular response to cold GO:0070417 ISS
    negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation GO:1903912 IMP
    negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation GO:1903912 ISS
Subcellular Localization
    extracellular region GO:0005576 TAS
    cytoplasm GO:0005737 TAS
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 ISS
    endoplasmic reticulum lumen GO:0005788 TAS
    smooth endoplasmic reticulum GO:0005790 IEA
    cytosol GO:0005829 ISS
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
    azurophil granule lumen GO:0035578 TAS
    extracellular exosome GO:0070062 HDA
    extracellular vesicle GO:1903561 HDA
 Experiment description of studies that identified DNAJC3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
8
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DNAJC3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CNPY2 10330
Proximity Label-MS Homo sapiens
2 UGGT2 55757
Proximity Label-MS Homo sapiens
3 TOR1A 1861
Proximity Label-MS Homo sapiens
4 NUCB2 4925
Proximity Label-MS Homo sapiens
5 SP1  
Co-fractionation Homo sapiens
6 DNAJA4 55466
Affinity Capture-MS Homo sapiens
7 WNT7A  
Affinity Capture-MS Homo sapiens
8 ERAP2 64167
Proximity Label-MS Homo sapiens
9 UBA1 7317
Co-fractionation Homo sapiens
10 HSPA1L 3305
Affinity Capture-MS Homo sapiens
11 MFAP4 4239
Affinity Capture-MS Homo sapiens
12 ECEL1  
Affinity Capture-MS Homo sapiens
13 PLD4  
Affinity Capture-MS Homo sapiens
14 ROCK2 9475
Co-fractionation Homo sapiens
15 GLB1L2  
Affinity Capture-MS Homo sapiens
16 GRAMD1A  
Proximity Label-MS Homo sapiens
17 HNRNPR 10236
Co-fractionation Homo sapiens
18 PARK7 11315
Proximity Label-MS Homo sapiens
19 DNAJB6 10049
Affinity Capture-MS Homo sapiens
20 HSPA4 3308
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
21 TCN2 6948
Affinity Capture-MS Homo sapiens
22 LAMC1 3915
Proximity Label-MS Homo sapiens
23 PLOD3 8985
Proximity Label-MS Homo sapiens
24 B4GALT4 8702
Affinity Capture-MS Homo sapiens
25 B3GNT2 10678
Affinity Capture-MS Homo sapiens
26 HLA-DRB3 3125
Affinity Capture-MS Homo sapiens
27 IMPDH2 3615
Proximity Label-MS Homo sapiens
28 PCK1 5105
Affinity Capture-MS Homo sapiens
29 CLEC18A  
Affinity Capture-MS Homo sapiens
30 TMEM231 79583
Affinity Capture-MS Homo sapiens
31 TRDN 10345
Cross-Linking-MS (XL-MS) Homo sapiens
32 TOR1AIP1 26092
Proximity Label-MS Homo sapiens
33 NUP210 23225
Proximity Label-MS Homo sapiens
34 HSPH1 10808
Co-fractionation Homo sapiens
35 ZFYVE1 53349
Affinity Capture-MS Homo sapiens
36 DDOST 1650
Proximity Label-MS Homo sapiens
37 PLCG1 5335
Co-fractionation Homo sapiens
38 DEDD  
Affinity Capture-MS Homo sapiens
39 DNAJC16  
Affinity Capture-MS Homo sapiens
40 PDIA6 10130
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
41 GUSB 2990
Proximity Label-MS Homo sapiens
42 DNAJC7 7266
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 PRDX4 10549
Proximity Label-MS Homo sapiens
44 CALR 811
Proximity Label-MS Homo sapiens
45 FKBP10 60681
Proximity Label-MS Homo sapiens
46 MLEC 9761
Proximity Label-MS Homo sapiens
47 PCNA 5111
Co-fractionation Homo sapiens
48 HSPA8 3312
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
49 EMC1 23065
Proximity Label-MS Homo sapiens
50 LMAN1 3998
Proximity Label-MS Homo sapiens
51 LRRC59 55379
Proximity Label-MS Homo sapiens
52 EIF2AK2 5610
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
53 EIF2AK3  
Affinity Capture-Western Homo sapiens
Phenotypic Suppression Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
54 PPIB 5479
Proximity Label-MS Homo sapiens
55 P4HA1 5033
Proximity Label-MS Homo sapiens
56 DNAJB1 3337
Co-fractionation Homo sapiens
57 ERO1L 30001
Proximity Label-MS Homo sapiens
58 IKBIP 121457
Proximity Label-MS Homo sapiens
59 FUT8 2530
Affinity Capture-MS Homo sapiens
60 TMX1 81542
Proximity Label-MS Homo sapiens
61 INPP4B 8821
Co-fractionation Homo sapiens
62 PARK2  
Affinity Capture-MS Homo sapiens
63 FCN3 8547
Affinity Capture-MS Homo sapiens
64 HSP90B1 7184
Proximity Label-MS Homo sapiens
65 TMTC3 160418
Proximity Label-MS Homo sapiens
66 PLOD2 5352
Proximity Label-MS Homo sapiens
67 KDELC2 143888
Proximity Label-MS Homo sapiens
68 IDS  
Affinity Capture-MS Homo sapiens
69 CLCC1 23155
Proximity Label-MS Homo sapiens
70 SDF2L1 23753
Proximity Label-MS Homo sapiens
71 OGT 8473
Reconstituted Complex Homo sapiens
72 HIST1H1E 3008
Cross-Linking-MS (XL-MS) Homo sapiens
73 DNAJB9 4189
Affinity Capture-MS Homo sapiens
74 ERP29 10961
Proximity Label-MS Homo sapiens
75 MIA3 375056
Proximity Label-MS Homo sapiens
76 MOGS 7841
Proximity Label-MS Homo sapiens
77 CDHR4  
Affinity Capture-MS Homo sapiens
78 P4HB 5034
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
79 RPN1 6184
Proximity Label-MS Homo sapiens
80 GANAB 23193
Proximity Label-MS Homo sapiens
81 WNT10A  
Affinity Capture-MS Homo sapiens
82 OLFML2B 25903
Affinity Capture-MS Homo sapiens
83 DNAJB5  
Affinity Capture-MS Homo sapiens
84 PTPRO 5800
Affinity Capture-MS Homo sapiens
85 SEC61B 10952
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
86 DNAJB11 51726
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
87 P3H1 64175
Proximity Label-MS Homo sapiens
88 ARSA 410
Affinity Capture-MS Homo sapiens
89 SEC63 11231
Proximity Label-MS Homo sapiens
90 HSPA5 3309
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
91 NOMO2 283820
Proximity Label-MS Homo sapiens
92 PCDHB7  
Affinity Capture-MS Homo sapiens
93 HIST1H3A 8350
Cross-Linking-MS (XL-MS) Homo sapiens
94 KMT2A  
Cross-Linking-MS (XL-MS) Homo sapiens
95 CANX 821
Proximity Label-MS Homo sapiens
96 DNAJB12 54788
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 CALR3  
Proximity Label-MS Homo sapiens
98 TXNDC5 81567
Proximity Label-MS Homo sapiens
99 CCT6A 908
Co-fractionation Homo sapiens
100 GGH 8836
Affinity Capture-MS Homo sapiens
101 PON2 5445
Affinity Capture-MS Homo sapiens
102 TENM3 55714
Affinity Capture-MS Homo sapiens
103 SLC25A31 83447
Cross-Linking-MS (XL-MS) Homo sapiens
104 BCAP31 10134
Proximity Label-MS Homo sapiens
105 PRSS50  
Affinity Capture-MS Homo sapiens
106 HSPA14 51182
Co-fractionation Homo sapiens
107 PDIA3 2923
Proximity Label-MS Homo sapiens
108 FKBP8 23770
Proximity Label-MS Homo sapiens
109 RCN1 5954
Proximity Label-MS Homo sapiens
110 HEXA 3073
Co-fractionation Homo sapiens
111 EPHA7 2045
Affinity Capture-MS Homo sapiens
112 UGGT1 56886
Proximity Label-MS Homo sapiens
113 AAR2 25980
Cross-Linking-MS (XL-MS) Homo sapiens
114 IL12RB1  
Affinity Capture-MS Homo sapiens
115 EMC2 9694
Proximity Label-MS Homo sapiens
116 ATP6V1A 523
Co-fractionation Homo sapiens
117 NDC80 10403
Co-fractionation Homo sapiens
118 DNAJC10 54431
Proximity Label-MS Homo sapiens
119 HYOU1 10525
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
120 KTN1 3895
Cross-Linking-MS (XL-MS) Homo sapiens
121 PLD3 23646
Proximity Label-MS Homo sapiens
122 HSPA9 3313
Co-fractionation Homo sapiens
123 LMNA 4000
Co-fractionation Homo sapiens
124 AKAP1 8165
Proximity Label-MS Homo sapiens
125 GSR 2936
Proximity Label-MS Homo sapiens
126 TTC13  
Proximity Label-MS Homo sapiens
127 KRT18 3875
Co-fractionation Homo sapiens
128 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
129 PRKCSH 5589
Proximity Label-MS Homo sapiens
130 ACP1 52
Proximity Label-MS Homo sapiens
131 SIAE 54414
Affinity Capture-MS Homo sapiens
132 PDIA4 9601
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
133 DNAJA2 10294
Affinity Capture-MS Homo sapiens
134 CALU 813
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
135 EMC7 56851
Proximity Label-MS Homo sapiens
136 HNRNPA2B1 3181
Cross-Linking-MS (XL-MS) Homo sapiens
137 EDEM1  
Affinity Capture-MS Homo sapiens
138 MGAT5 4249
Affinity Capture-MS Homo sapiens
139 GRPEL1 80273
Co-fractionation Homo sapiens
140 PLOD1 5351
Proximity Label-MS Homo sapiens
141 WNT10B 7480
Affinity Capture-MS Homo sapiens
142 CREB1  
Co-fractionation Homo sapiens
143 TUBB4A 10382
Affinity Capture-MS Homo sapiens
144 SFTPC  
Affinity Capture-MS Homo sapiens
145 CNTRL  
Cross-Linking-MS (XL-MS) Homo sapiens
146 NUTM2F  
Affinity Capture-MS Homo sapiens
147 PYGM 5837
Affinity Capture-MS Homo sapiens
148 LONP1 9361
Co-fractionation Homo sapiens
149 LRPAP1 4043
Proximity Label-MS Homo sapiens
150 BRICD5  
Affinity Capture-MS Homo sapiens
151 HNRNPH3 3189
Co-fractionation Homo sapiens
152 WNT3A 89780
Affinity Capture-MS Homo sapiens
153 ADGRV1 84059
Affinity Capture-MS Homo sapiens
154 MAGT1 84061
Proximity Label-MS Homo sapiens
155 STT3B 201595
Proximity Label-MS Homo sapiens
156 DNAJC1 64215
Affinity Capture-MS Homo sapiens
157 COLGALT1 79709
Proximity Label-MS Homo sapiens
158 NFIC 4782
Co-fractionation Homo sapiens
159 CKAP4 10970
Proximity Label-MS Homo sapiens
160 TRGV3  
Affinity Capture-MS Homo sapiens
161 PRKRIR  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
162 PCDHB3  
Affinity Capture-MS Homo sapiens
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