Gene description for P4HA1
Gene name prolyl 4-hydroxylase, alpha polypeptide I
Gene symbol P4HA1
Other names/aliases P4HA
Species Homo sapiens
 Database cross references - P4HA1
ExoCarta ExoCarta_5033
Entrez Gene 5033
HGNC 8546
MIM 176710
UniProt P13674  
 P4HA1 identified in exosomes derived from the following tissue/cell type
Squamous carcinoma cells 20124223    
 Gene ontology annotations for P4HA1
Molecular Function
    procollagen-proline 4-dioxygenase activity GO:0004656 IEA
    protein binding GO:0005515 IPI
    L-ascorbic acid binding GO:0031418 IEA
    oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen GO:0016702 IEA
    iron ion binding GO:0005506 IEA
Biological Process
    oxidation-reduction process GO:0055114 IEA
    collagen fibril organization GO:0030199 IEA
    peptidyl-proline hydroxylation to 4-hydroxy-L-proline GO:0018401 IEA
Subcellular Localization
    mitochondrion GO:0005739 IDA
    membrane GO:0016020 IDA
    endoplasmic reticulum lumen GO:0005788 IEA
    procollagen-proline 4-dioxygenase complex GO:0016222 IEA
    endoplasmic reticulum GO:0005783 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified P4HA1 in exosomes
1
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for P4HA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which P4HA1 is involved
No pathways found





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