Gene description for COL16A1
Gene name collagen, type XVI, alpha 1
Gene symbol COL16A1
Other names/aliases 447AA
FP1572
Species Homo sapiens
 Database cross references - COL16A1
ExoCarta ExoCarta_1307
Entrez Gene 1307
HGNC 2193
MIM 120326
UniProt Q07092  
 COL16A1 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for COL16A1
Molecular Function
    protein binding GO:0005515 IPI
    integrin binding GO:0005178 IDA
Biological Process
    integrin activation GO:0033622 IDA
    extracellular matrix disassembly GO:0022617 TAS
    cellular response to amino acid stimulus GO:0071230 IEA
    collagen catabolic process GO:0030574 TAS
    cell adhesion GO:0007155 IDA
    female pregnancy GO:0007565 TAS
    positive regulation of focal adhesion assembly GO:0051894 IDA
    cell adhesion mediated by integrin GO:0033627 IDA
    extracellular matrix organization GO:0030198 TAS
    integrin-mediated signaling pathway GO:0007229 TAS
Subcellular Localization
    endoplasmic reticulum lumen GO:0005788 TAS
    proteinaceous extracellular matrix GO:0005578 IEA
    collagen type XVI trimer GO:0005597 TAS
    extracellular region GO:0005576 TAS
 Experiment description of studies that identified COL16A1 in exosomes
1
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 193
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
PHB
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 194
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 195
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for COL16A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which COL16A1 is involved
PathwayEvidenceSource
Collagen biosynthesis and modifying enzymes TAS Reactome
Collagen degradation TAS Reactome
Integrin cell surface interactions TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here