Gene description for PDHB
Gene name pyruvate dehydrogenase (lipoamide) beta
Gene symbol PDHB
Other names/aliases PDHBD
PDHE1-B
PHE1B
Species Homo sapiens
 Database cross references - PDHB
ExoCarta ExoCarta_5162
Entrez Gene 5162
HGNC 8808
MIM 179060
UniProt P11177  
 PDHB identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for PDHB
Molecular Function
    pyruvate dehydrogenase (acetyl-transferring) activity GO:0004739 IEA
    pyruvate dehydrogenase activity GO:0004738 IDA
    protein binding GO:0005515 IPI
Biological Process
    glucose metabolic process GO:0006006 IEA
    regulation of acetyl-CoA biosynthetic process from pyruvate GO:0010510 TAS
    tricarboxylic acid cycle GO:0006099 IDA
    pyruvate metabolic process GO:0006090 TAS
    acetyl-CoA biosynthetic process from pyruvate GO:0006086 IDA
    small molecule metabolic process GO:0044281 TAS
    cellular metabolic process GO:0044237 TAS
Subcellular Localization
    mitochondrial matrix GO:0005759 TAS
    nucleoplasm GO:0005654 IDA
    mitochondrion GO:0005739 IDA
    extracellular exosome GO:0070062 IDA
    nucleus GO:0005634 IDA
    pyruvate dehydrogenase complex GO:0045254 IDA
 Experiment description of studies that identified PDHB in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for PDHB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PDHX  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
2 DLAT 1737
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
3 PDHA2  
Invivo Homo sapiens
4 PDHA1 5160
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
5 PDHB 5162
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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