|
 Gene description for PCYT2 |
| Gene name |
phosphate cytidylyltransferase 2, ethanolamine |
| Gene symbol |
PCYT2 |
| Other names/aliases |
ET |
| Species |
Homo sapiens |
| Database cross references - PCYT2 |
| ExoCarta |
ExoCarta_5833 |
| Entrez Gene |
5833 |
| HGNC |
8756 |
| MIM |
602679 |
| UniProt |
Q99447
|
| PCYT2 identified in exosomes derived from the following tissue/cell type |
|
Ovarian cancer cells
|
23333927
|
|
Ovarian cancer cells
|
23333927
|
|
Thymus
|
23844026
|
| Gene ontology annotations for PCYT2 |
|
|
| Experiment description of studies that identified PCYT2 in exosomes |
| 1 |
| Experiment ID |
211 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|Alix
|
EV Cytosolic markers |
|
✔
EpCAM|TFRC
|
EV Membrane markers |
|
✔
cytochrome c|GOLGA2
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23333927
|
| Organism |
Homo sapiens |
| Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
| Authors |
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. |
| Journal name |
J Proteomics
|
| Publication year |
2013 |
| Sample |
Ovarian cancer cells |
| Sample name |
IGROV1 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.09-1.15 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 2 |
| Experiment ID |
212 |
| ISEV standards |
|
✔
CEM
|
EV Biophysical techniques |
|
✔
TSG101|Alix
|
EV Cytosolic markers |
|
✔
EpCAM|TFRC
|
EV Membrane markers |
|
✔
Cytochrome C|GOLGA2
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23333927
|
| Organism |
Homo sapiens |
| Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
| Authors |
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. |
| Journal name |
J Proteomics
|
| Publication year |
2013 |
| Sample |
Ovarian cancer cells |
| Sample name |
OVCAR-3 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.09-1.15 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 3 |
| Experiment ID |
217 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101
|
EV Cytosolic markers |
|
✔
CD81|CD9|CD63
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23844026
|
| Organism |
Homo sapiens |
| Experiment description |
Characterization of human thymic exosomes. |
| Authors |
Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O. |
| Journal name |
PLoS One
|
| Publication year |
2013 |
| Sample |
Thymus |
| Sample name |
Normal-Thymus |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| Protein-protein interactions for PCYT2 |
|
|
| Pathways in which PCYT2 is involved |
|
|
|
